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Article

Characterization of Anti-Ana o 3 Monoclonal Antibodies and Their Application in Comparing Brazilian Cashew Cultivars

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Southern Regional Research Center, FPSQ, ARS, U.S. Department of Agriculture, New Orleans, LA 70124, USA
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Contiguity Consulting, Boulder, CO 80302, USA
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Embrapa Agroindústria Tropical, Fortaleza 60511-110, CE, Brazil
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BioMed Bridge LLC., 3700 Quebec Street, Suite 100-230, Denver, CO 80207, USA
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Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA
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New Orleans Louisiana Neuroendocrine Tumor Specialists (NOLANETS), Ochsner Medical Center, Kenner, LA 70065, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Itai Benhar
Received: 3 August 2021 / Revised: 11 October 2021 / Accepted: 29 October 2021 / Published: 28 November 2021
Ana o 3 is an immuno-dominant cashew nut allergen. Four monoclonal antibodies to Ana o 3 (2H5, 6B9C1, 19C9A2, and 5B7F8) were characterized by ELISA and in silico modeling. The 2H5 antibody was the only antibody specific for cashew nut extract. In addition to cashew nut extract, the 6B9C1 and 19C9A2 antibodies recognized pistachio extract, and the 5B7F8 recognized pecan extract. All four antibodies recognized both recombinant Ana o 3.0101 and native Ana o 3. ELISA assays following treatment of purified Ana o 3 with a reducing agent indicated that the 6B9C1 and 19C9A2 antibodies likely recognize conformational epitopes, while the 2H5 and 5B7F8 antibodies likely recognize linear epitopes. In silico modeling predicted distinct epitopes for each of the anti-Ana o 3 antibodies. Screening extracts from 11 Brazilian cashew nut cultivars using all four antibodies showed slight differences in Ana o 3 bindings, demonstrating that these antibodies could identify cultivars with varying allergen content. View Full-Text
Keywords: cashew nut; allergy; Ana o 3; antibody; ELISA; cross-reactive cashew nut; allergy; Ana o 3; antibody; ELISA; cross-reactive
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MDPI and ACS Style

Mattison, C.P.; Vant-Hull, B.; de Castro, A.C.R.; Chial, H.J.; Bren-Mattison, Y.; Bechtel, P.J.; de Brito, E.S. Characterization of Anti-Ana o 3 Monoclonal Antibodies and Their Application in Comparing Brazilian Cashew Cultivars. Antibodies 2021, 10, 46. https://0-doi-org.brum.beds.ac.uk/10.3390/antib10040046

AMA Style

Mattison CP, Vant-Hull B, de Castro ACR, Chial HJ, Bren-Mattison Y, Bechtel PJ, de Brito ES. Characterization of Anti-Ana o 3 Monoclonal Antibodies and Their Application in Comparing Brazilian Cashew Cultivars. Antibodies. 2021; 10(4):46. https://0-doi-org.brum.beds.ac.uk/10.3390/antib10040046

Chicago/Turabian Style

Mattison, Christopher P., Barry Vant-Hull, Ana C.R. de Castro, Heidi J. Chial, Yvette Bren-Mattison, Peter J. Bechtel, and Edy S. de Brito. 2021. "Characterization of Anti-Ana o 3 Monoclonal Antibodies and Their Application in Comparing Brazilian Cashew Cultivars" Antibodies 10, no. 4: 46. https://0-doi-org.brum.beds.ac.uk/10.3390/antib10040046

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