Design and Testing of Effective Primers for Amplification of the orf7 Gene of Phage WO Associated with Andricus hakonensis
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Insects and Total Genomic DNA Extraction
2.2. PCR and Sequencing
2.3. Raw Sequence Treatment
2.4. Phylogenetic Analyses
2.5. RNA Extraction
3. Results
3.1. Acquisition and Typing of the Full-Length Sequence of the Phage WO orf7 Gene
3.2. Design of Highly Effective Primers for virus Amplification
3.3. Base Deletions in orf7
3.4. A High Proportion of Phage WO Types Are Actively Transcribed in A. hakonensis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Primer | Primer Sequence | Annealing Temperature | References | |
---|---|---|---|---|---|
Phage WO | orf7 | WO-F WO-R | 5′-CCC ACA TGA GCC AAT GAC GTC TG-3′ 5′-CGT TCG CTC TGC AAG TAA CTC CAT TAA AAC-3′ | 57 °C | Masui et al., 2000 |
WO-TF WO-TR | 5′-CTTGGCTACYGTTATYTGRTCTCTTG-3′ 5′-TCYGASGTTACTRCHAATCAAGAGG-3′ | 59 °C | This study | ||
WO-SUF: WO-SUR: | 5′-ARGCAGGKCTWTATTTTGG-3′ 5′-ATTCTTCTATYTTYYCTGGCA-3′ | 50 °C | This study | ||
Wolbachia | wsp | 81F 691R | 5′-TGG TCC AAT AAG TGA TGA AGA AAC-3′ 5′-AAA AAT TAA ACG CTA CTC CA-3′ | 52 °C | Zhou et al.,1998 |
Insect | ITS2 | ITS2F ITS2R | 5′-TGT GAA CTG CAG GAC ACA TG-3′ 5′-AAT GCT TAA ATT TAG GGG GTA-3′ | 54 °C | Partensky and Garczarek, 2011 |
cox1 | LCO-1490 HCO-2198 | 5′-GGTCAACAAATCATAAAGATATTGG-3′ 5′-TAAACTTCAGGGTGACCAAAAATCA-3′ | 55 °C | Dyer et al., 2011 |
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Su, C.-Y.; Zhu, D.-H.; Yang, X.-H. Design and Testing of Effective Primers for Amplification of the orf7 Gene of Phage WO Associated with Andricus hakonensis. Insects 2021, 12, 713. https://0-doi-org.brum.beds.ac.uk/10.3390/insects12080713
Su C-Y, Zhu D-H, Yang X-H. Design and Testing of Effective Primers for Amplification of the orf7 Gene of Phage WO Associated with Andricus hakonensis. Insects. 2021; 12(8):713. https://0-doi-org.brum.beds.ac.uk/10.3390/insects12080713
Chicago/Turabian StyleSu, Cheng-Yuan, Dao-Hong Zhu, and Xiao-Hui Yang. 2021. "Design and Testing of Effective Primers for Amplification of the orf7 Gene of Phage WO Associated with Andricus hakonensis" Insects 12, no. 8: 713. https://0-doi-org.brum.beds.ac.uk/10.3390/insects12080713