Application of the High-Throughput TAB-Array for the Discovery of Novel 5-Hydroxymethylcytosine Biomarkers in Pancreatic Ductal Adenocarcinoma
Abstract
:1. Introduction
2. Results
2.1. Genomic Distributions of 5hmC
2.2. PDAC-Associated 5hmC Loci
2.3. Exploring Functional Relevance, Prognostic Value, and Cancer Specificity
3. Discussion
4. Materials and Methods
4.1. Study Subjects
4.2. DNA Isolation and the TAB-Array Assay
4.3. Processing of the TAB-Array Data and Detection of Differential Loci
4.4. Evaluating Genomic Distributions of 5hmC and Functional Relevance
4.5. Exploring Prognostic Value and Pancreatic Cancer-Specific Expression of PDAC-Associated 5hmC Loci
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Zeng, C.; Zhang, Z.; Wang, J.; Chiu, B.C.-H.; Hou, L.; Zhang, W. Application of the High-Throughput TAB-Array for the Discovery of Novel 5-Hydroxymethylcytosine Biomarkers in Pancreatic Ductal Adenocarcinoma. Epigenomes 2019, 3, 16. https://0-doi-org.brum.beds.ac.uk/10.3390/epigenomes3030016
Zeng C, Zhang Z, Wang J, Chiu BC-H, Hou L, Zhang W. Application of the High-Throughput TAB-Array for the Discovery of Novel 5-Hydroxymethylcytosine Biomarkers in Pancreatic Ductal Adenocarcinoma. Epigenomes. 2019; 3(3):16. https://0-doi-org.brum.beds.ac.uk/10.3390/epigenomes3030016
Chicago/Turabian StyleZeng, Chang, Zhou Zhang, Jun Wang, Brian C-H Chiu, Lifang Hou, and Wei Zhang. 2019. "Application of the High-Throughput TAB-Array for the Discovery of Novel 5-Hydroxymethylcytosine Biomarkers in Pancreatic Ductal Adenocarcinoma" Epigenomes 3, no. 3: 16. https://0-doi-org.brum.beds.ac.uk/10.3390/epigenomes3030016