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Genome-Wide DNA Methylation and LncRNA-Associated DNA Methylation in Metformin-Treated and -Untreated Diabetes

1
SAMRC/CPUT/Cardiometabolic Health Research Unit, Department of Biomedical Sciences, Faculty of Health and Wellness Science, Cape Peninsula University of Technology, P.O. Box 1906, Bellville, Cape Town 7530, South Africa
2
Division of Chemical Pathology, Faculty of Health Sciences, National Health Laboratory Service (NHLS) and University of Stellenbosch, Cape Town 7505, South Africa
3
Non-Communicable Diseases Research Unit, South African Medical Research Council, Cape Town 7505, South Africa
4
Department of Medicine, University of Cape Town, Cape Town 7700, South Africa
*
Author to whom correspondence should be addressed.
Received: 8 July 2020 / Revised: 3 August 2020 / Accepted: 14 August 2020 / Published: 1 September 2020
(This article belongs to the Special Issue Complex Disease Epigenetics)
Metformin, which is used as a first line treatment for type 2 diabetes mellitus (T2DM), has been shown to affect epigenetic patterns. In this study, we investigated the DNA methylation and potential lncRNA modifications in metformin-treated and newly diagnosed adults with T2DM. Genome-wide DNA methylation and lncRNA analysis were performed from the peripheral blood of 12 screen-detected and 12 metformin-treated T2DM individuals followed by gene ontology (GO) and KEGG pathway analysis. Differentially methylated regions (DMRs) observed showed 22 hypermethylated and 11 hypomethylated DMRs between individuals on metformin compared to screen-detected subjects. Amongst the hypomethylated DMR regions were the SLC gene family, specifically, SLC25A35 and SLC28A1. Fifty-seven lncRNA-associated DNA methylation regions included the mitochondrial ATP synthase-coupling factor 6 (ATP5J). Functional gene mapping and pathway analysis identified regions in the axon initial segment (AIS), node of Ranvier, cell periphery, cleavage furrow, cell surface furrow, and stress fiber. In conclusion, our study has identified a number of DMRs and lncRNA-associated DNA methylation regions in metformin-treated T2DM that are potential targets for therapeutic monitoring in patients with diabetes. View Full-Text
Keywords: metformin; DNA methylation; lncRNA; diabetes mellitus; Africa metformin; DNA methylation; lncRNA; diabetes mellitus; Africa
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MDPI and ACS Style

Solomon, W.L.; Hector, S.B.E.; Raghubeer, S.; Erasmus, R.T.; Kengne, A.P.; Matsha, T.E. Genome-Wide DNA Methylation and LncRNA-Associated DNA Methylation in Metformin-Treated and -Untreated Diabetes. Epigenomes 2020, 4, 19. https://0-doi-org.brum.beds.ac.uk/10.3390/epigenomes4030019

AMA Style

Solomon WL, Hector SBE, Raghubeer S, Erasmus RT, Kengne AP, Matsha TE. Genome-Wide DNA Methylation and LncRNA-Associated DNA Methylation in Metformin-Treated and -Untreated Diabetes. Epigenomes. 2020; 4(3):19. https://0-doi-org.brum.beds.ac.uk/10.3390/epigenomes4030019

Chicago/Turabian Style

Solomon, Wendy L.; Hector, Stanton B.E.; Raghubeer, Shanel; Erasmus, Rajiv T.; Kengne, Andre P.; Matsha, Tandi E. 2020. "Genome-Wide DNA Methylation and LncRNA-Associated DNA Methylation in Metformin-Treated and -Untreated Diabetes" Epigenomes 4, no. 3: 19. https://0-doi-org.brum.beds.ac.uk/10.3390/epigenomes4030019

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