Next Article in Journal
Epigenetic Regulation of Epidermal Differentiation
Previous Article in Journal
Combinatorial Epigenetic and Immunotherapy in Breast Cancer Management: A Literature Review
Open AccessArticle

Methylomes in Vegans versus Pescatarians and Nonvegetarians

1
Department of Basic Sciences, School of Medicine, Loma Linda University, Loma Linda, CA 92354, USA
2
Department of Nutrition, School of Public Health, Loma Linda University, Loma Linda, CA 92354, USA
3
Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA 92037, USA
*
Author to whom correspondence should be addressed.
Received: 30 September 2020 / Revised: 7 December 2020 / Accepted: 8 December 2020 / Published: 11 December 2020
Epigenetic studies in animal models have demonstrated that diet affects gene regulation by altering methylation patterns. We interrogated methylomes in humans who have different sources of protein in their diet. We compared methylation of DNA isolated from buffy coat in 38 vegans, 41 pescatarians and 68 nonvegetarians. Methylation data were obtained using Infinium HumanMethylation450 arrays and analyzed using the Partek Genomic software. Differences in differentially methylated sites were small, though with the use of relaxed statistical tests we did identify diet-associated differences. To further test the validity of these observations, we performed separate and independent comparisons of the methylation differences between vegans and nonvegetarians, and between vegans and pescatarians. The detected differences were then examined to determine if they were enriched in specific pathways. Pathway analysis revealed enrichment of several specific processes, including homeobox transcription and glutamate transport. The detected differences in DNA methylation patterns between vegans, pescatarians, and nonvegetarians enabled us to identify 77 CpG sites that may be sensitive to diet and/or lifestyle, though high levels of individual-specific differences were also noted. View Full-Text
Keywords: differential DNA methylation; epigenetics; pathway analysis; vegan diet differential DNA methylation; epigenetics; pathway analysis; vegan diet
Show Figures

Figure 1

MDPI and ACS Style

Filippov, V.; Jaceldo-Siegl, K.; Eroshkin, A.; Loskutov, V.; Chen, X.; Wang, C.; Duerksen-Hughes, P.J. Methylomes in Vegans versus Pescatarians and Nonvegetarians. Epigenomes 2020, 4, 28. https://0-doi-org.brum.beds.ac.uk/10.3390/epigenomes4040028

AMA Style

Filippov V, Jaceldo-Siegl K, Eroshkin A, Loskutov V, Chen X, Wang C, Duerksen-Hughes PJ. Methylomes in Vegans versus Pescatarians and Nonvegetarians. Epigenomes. 2020; 4(4):28. https://0-doi-org.brum.beds.ac.uk/10.3390/epigenomes4040028

Chicago/Turabian Style

Filippov, Valery; Jaceldo-Siegl, Karen; Eroshkin, Alexey; Loskutov, Vasiliy; Chen, Xin; Wang, Charles; Duerksen-Hughes, Penelope J. 2020. "Methylomes in Vegans versus Pescatarians and Nonvegetarians" Epigenomes 4, no. 4: 28. https://0-doi-org.brum.beds.ac.uk/10.3390/epigenomes4040028

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop