Next Article in Journal
Probiotics Enhance Cereal Yield and Quality and Modify Agrochemical Soil Properties
Previous Article in Journal
Concurrent and Subsequent Co-Infections of Clostridioides difficile Colitis in the Era of Gut Microbiota and Expanding Treatment Options
Previous Article in Special Issue
Putrescine Production by Latilactobacillus curvatus KP 3-4 Isolated from Fermented Foods
Article

Effects of Spore-Displayed p75 Protein from Lacticaseibacillus rhamnosus GG on the Transcriptional Response of HT-29 Cells

Department of Food Science and Biotechnology, College of Life Science and Biotechnology, Dongguk University, Goyang-si 10326, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Gianluigi Mauriello
Received: 30 May 2022 / Revised: 20 June 2022 / Accepted: 22 June 2022 / Published: 23 June 2022
(This article belongs to the Special Issue Probiotics and Their Metabolism)
A Lacticaseibacillus rhamnosus GG-derived protein, p75, is one of the key molecules exhibiting probiotic activity. However, the molecular mechanism and transcriptional response of p75 in human intestinal epithelial cells are not completely understood. To gain a deeper understanding of its potential probiotic action, this study investigated genome-wide responses of HT-29 cells to stimulation by spore-displayed p75 (CotG-p75) through a transcriptome analysis based on RNA sequencing. Analysis of RNA-seq data showed significant changes of gene expression in HT-29 cells stimulated by CotG-p75 compared to the control. A total of 189 up-regulated and 314 down-regulated genes was found as differentially expressed genes. Gene ontology enrichment analysis revealed that a large number of activated genes was involved in biological processes, such as epithelial cell differentiation, development, and regulation of cell proliferation. A gene–gene interaction network analysis showed that several DEGs, including AREG, EREG, HBEGF, EPGN, FASLG, GLI2, CDKN1A, FOSL1, MYC, SERPINE1, TNFSF10, BCL6, FLG, IVL, SPRR1A, SPRR1B, SPRR3, and MUC5AC, might play a critical role in these biological processes. RNA-seq results for selected genes were verified by reverse transcription-quantitative polymerase chain reaction. Overall, these results provide extensive knowledge about the transcriptional responses of HT-29 cells to stimulation by CotG-p75. This study showed that CotG-p75 can contribute to cell survival and epithelial development in human intestinal epithelial cells. View Full-Text
Keywords: p75 protein; postbiotics; spore surface display; RNA sequencing; HT-29 cells p75 protein; postbiotics; spore surface display; RNA sequencing; HT-29 cells
Show Figures

Figure 1

MDPI and ACS Style

Kang, S.-J.; Kim, M.-J.; Son, D.-Y.; Kang, S.-S.; Hong, K.-W. Effects of Spore-Displayed p75 Protein from Lacticaseibacillus rhamnosus GG on the Transcriptional Response of HT-29 Cells. Microorganisms 2022, 10, 1276. https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms10071276

AMA Style

Kang S-J, Kim M-J, Son D-Y, Kang S-S, Hong K-W. Effects of Spore-Displayed p75 Protein from Lacticaseibacillus rhamnosus GG on the Transcriptional Response of HT-29 Cells. Microorganisms. 2022; 10(7):1276. https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms10071276

Chicago/Turabian Style

Kang, Soo-Ji, Min-Joo Kim, Do-Yeong Son, Seok-Seong Kang, and Kwang-Won Hong. 2022. "Effects of Spore-Displayed p75 Protein from Lacticaseibacillus rhamnosus GG on the Transcriptional Response of HT-29 Cells" Microorganisms 10, no. 7: 1276. https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms10071276

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop