Next Article in Journal
The Union Is Strength: The Synergic Action of Long Fatty Acids and a Bacteriophage against Xanthomonas campestris Biofilm
Next Article in Special Issue
Detection of SARS-CoV-2 and Other Infectious Agents in Lower Respiratory Tract Samples Belonging to Patients Admitted to Intensive Care Units of a Tertiary-Care Hospital, Located in an Epidemic Area, during the Italian Lockdown
Previous Article in Journal
Azole-Resistant Aspergillus fumigatus Harboring the TR34/L98H Mutation: First Report in Portugal in Environmental Samples
Previous Article in Special Issue
Higher Mortality and Intensive Care Unit Admissions in COVID-19 Patients with Liver Enzyme Elevations
Article

Performance of a Point-of-Care Test for the Rapid Detection of SARS-CoV-2 Antigen

1
Institute for Infection Medicine, Christian-Albrecht University and University Medical Center Schleswig-Holstein, Brunswiker Str. 4, 24105 Kiel, Germany
2
Labor Dr. Krause und Kollegen MVZ GmbH, Steenbeker Weg 23, 24106 Kiel, Germany
*
Author to whom correspondence should be addressed.
These first authors contributed equally.
Received: 30 November 2020 / Revised: 16 December 2020 / Accepted: 24 December 2020 / Published: 28 December 2020
(This article belongs to the Special Issue COVID-19: Focusing on Epidemiologic, Virologic, and Clinical Studies)
The rapid detection of infections caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is necessary in the ongoing pandemic. Antigen-specific point-of-care tests (POCT) may be useful for this purpose. Here, such a POCT (SARS-CoV-2 NADAL® COVID-19 Ag) was compared to a laboratory-developed triplex real-time polymerase chain reaction (RT-PCR) designed for the detection of viral nucleoprotein gene and two control targets. This RT-PCR served as a reference to investigate POCT sensitivity by re-testing upper respiratory tract (URT) samples (n = 124) exhibiting different SARS-CoV-2 loads in terms of RT-PCR threshold cycle (Ct) values. The optical intensities of the antigen bands were compared to the Ct values of the RT-PCR. The infectivity of various virus loads was estimated by inoculating Vero cells with URT samples (n = 64, Ct 17-34). POCT sensitivity varied from 100% (Ct < 25) to 73.1% (Ct ≤ 30); higher SARS-CoV-2 loads correlated with higher band intensities. All samples with a Ct > 30 were negative; among SARS-CoV-2 free samples (n = 10) no false-positives were detected. A head-to-head comparison with another POCT (Abbott, Panbio™ COVID-19 Ag Rapid Test) yielded similar results. Isolation of SARS-CoV-2 in cell-culture was successful up to a Ct value of 29. The POCT reliably detects high SARS-CoV-2 loads and rapidly identifies infectious individuals. View Full-Text
Keywords: COVID-19; diagnostic; PCR; antigen; POCT; comparison COVID-19; diagnostic; PCR; antigen; POCT; comparison
Show Figures

Figure 1

MDPI and ACS Style

Strömer, A.; Rose, R.; Schäfer, M.; Schön, F.; Vollersen, A.; Lorentz, T.; Fickenscher, H.; Krumbholz, A. Performance of a Point-of-Care Test for the Rapid Detection of SARS-CoV-2 Antigen. Microorganisms 2021, 9, 58. https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms9010058

AMA Style

Strömer A, Rose R, Schäfer M, Schön F, Vollersen A, Lorentz T, Fickenscher H, Krumbholz A. Performance of a Point-of-Care Test for the Rapid Detection of SARS-CoV-2 Antigen. Microorganisms. 2021; 9(1):58. https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms9010058

Chicago/Turabian Style

Strömer, Annabelle, Ruben Rose, Miriam Schäfer, Frieda Schön, Anna Vollersen, Thomas Lorentz, Helmut Fickenscher, and Andi Krumbholz. 2021. "Performance of a Point-of-Care Test for the Rapid Detection of SARS-CoV-2 Antigen" Microorganisms 9, no. 1: 58. https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms9010058

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop