Composition and Corrosivity of Extracellular Polymeric Substances from the Hydrocarbon-Degrading Sulfate-Reducing Bacterium Desulfoglaeba alkanexedens ALDC
Abstract
:1. Introduction
2. Materials and Methods
2.1. Incubations
2.2. EPS Extraction
2.3. Field Emission Scanning Electron Microscopy (FE-SEM)
2.4. Confocal Microscopy
2.5. Nuclear Magnetic Resonance (NMR) Analysis
2.6. Corrosion Analysis
2.7. Polysaccharide Characterization
3. Results
3.1. D. alkanexedens Aggregation and Extracellular Material
3.2. Fluorescent Microscopic Analysis of D. alkanexedens Aggregates
3.3. EPS Composition and Structure
3.4. Nuclear Magnetic Resonance Analysis
3.5. Assessment of D. alkanexedens EPS Corrosivity
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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1H NMR Chemical Shift Values (ppm) | |||
---|---|---|---|
Experimental NMR | Predicted NMR | Starch in D2O | |
H1 | 5.19 | 4.58 | 5.35 |
H2 | 3.66 | 3.24 | 3.63 |
H3 | 3.87 | 3.65 | 3.94 |
H4 | 3.47 | 3.49 | 3.62 |
H5 | 3.62 | 3.52 | 3.82 |
Hb | 2.10 | 2.32 | |
Ha Ha′ | 2.36 | 2.35, 2.45 | |
Hd Hd′ | 2.42 | 2.42, 2.35 | |
Hc Hc′ | 2.34 | 2.48, 2.27 | |
H1′ | 5.19 | 4.49 | 5.35 |
H2′ | 3.66 | 3.20 | 3.63 |
H3′ | 3.62 | 3.60 | 3.94 |
H4′ | 3.47 | 3.16 | 3.62 |
H5′ | 3.87 | 3.46 | 3.82 |
H6′ H6′ | 3.76 | 3.81, 3.81 | 3.87 |
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Davidova, I.A.; Lenhart, T.R.; Nanny, M.A.; Suflita, J.M. Composition and Corrosivity of Extracellular Polymeric Substances from the Hydrocarbon-Degrading Sulfate-Reducing Bacterium Desulfoglaeba alkanexedens ALDC. Microorganisms 2021, 9, 1994. https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms9091994
Davidova IA, Lenhart TR, Nanny MA, Suflita JM. Composition and Corrosivity of Extracellular Polymeric Substances from the Hydrocarbon-Degrading Sulfate-Reducing Bacterium Desulfoglaeba alkanexedens ALDC. Microorganisms. 2021; 9(9):1994. https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms9091994
Chicago/Turabian StyleDavidova, Irene A., Tiffany R. Lenhart, Mark A. Nanny, and Joseph M. Suflita. 2021. "Composition and Corrosivity of Extracellular Polymeric Substances from the Hydrocarbon-Degrading Sulfate-Reducing Bacterium Desulfoglaeba alkanexedens ALDC" Microorganisms 9, no. 9: 1994. https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms9091994