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Article

Assessment of the Levels of Pollution and of Their Risks by Radioactivity and Trace Metals on Marine Edible Fish and Crustaceans at the Bay of Bengal (Chattogram, Bangladesh)

1
Department of Physics, Chittagong University of Engineering and Technology, Chattogram 4349, Bangladesh
2
Atomic Energy Centre, Bangladesh Atomic Energy Commission, Chattogram 4209, Bangladesh
3
Interdisciplinary Graduate School of Engineering Sciences, Kyushu University, Fukuoka 816-8580, Japan
4
MARE—Marine and Environmental Sciences Centre, ESTM-School of Tourism and Maritime Technology, Polytechnic of Leiria, 2520-641 Peniche, Portugal
*
Authors to whom correspondence should be addressed.
Submission received: 10 November 2020 / Revised: 31 January 2021 / Accepted: 1 February 2021 / Published: 11 February 2021

Abstract

:
Marine environmental pollution is a longstanding global problem and has a particular impact on the Bay of Bengal. Effluent from different sources directly enters rivers of the region and eventually flows into the Bay of Bengal. This effluent may contain radioactive materials and trace metals and pose a serious threat to the coastal environment, in addition to aquatic ecosystems. Using gamma spectrometry and atomic absorption spectrometry, a comprehensive study was carried out on the radioactivity (226Ra, 232Th, 40K, and 137Cs) and trace metal (Cd, Pb, Zn, Cu, Ni, Fe, Mn, and Cr) concentrations, respectively, in fish and crustacean species collected from the coastal belt of the Bay of Bengal (Chattogram, Bangladesh). The analysis showed a noticeable increment in the levels of different radioactive pollutants in the marine samples, although the consumption of the studied fish and crustacean species should be considered safe for human health. Anthropogenic radionuclide (137Cs) was not detected in any sample. Furthermore, the metal concentrations of a small number of trace elements (Pb, Cd, Cr) were found to be higher in most of the samples, which indicates aquatic fauna are subject to pollution. The estimated daily intake (EDI), target hazard quotient (THQ), hazard index (HI), and target cancer risk (TR) were calculated and compared with the permissible safety limits. It was found that consuming the seafood from the Bay of Bengal may cause adverse health impacts if consumption and/or means of pollution are not controlled.

1. Introduction

The marine environment is one of the most important sources of life on Earth and performs a number of key environmental functions for the lives and livelihood of humans and other organisms. Marine environmental pollution is a longstanding global problem. Marine pollution affects the Bay of Bengal because of the vulnerability of its aquatic habitats to such pollution following the industrial revolution in the 19th century. Most aquatic ecosystems can cope with a certain degree of pollution, but severe pollution is reflected in changes in the fauna and flora of their communities [1]. Developing countries, including Bangladesh, are most affected by this human-made problem. Bangladesh is considered to be one of the most suitable countries for the Blue Economy following the recent establishment of a vast maritime boundary in the Bay of Bengal. The result of two verdicts on maritime boundaries, also involving Myanmar and India, allows Bangladesh to exclusively exercise its sovereign rights over 118,813 sq km of water. The affected area extends up to 12 nautical miles into territorial sea and includes a further Exclusive Economic Zone (EEZ) of 200 nautical miles [2]. However, agriculture runoff, untreated sewage, and industrial pollution are reportedly being discharged directly into rivers, eventually flowing into the Bay of Bengal. This effluent may contain radioactive materials and trace metals and pose a particularly serious threat to the coastal environment and the aquatic ecosystems [3,4].
The main causes of radioactivity in the marine environment are seabed movement originating from underwater volcanic activity and undersea earthquakes, natural processes of weathering, and mineral recycling of terrestrial rocks [5]. Different types of geological materials and minerals, such as ores and igneous rocks, which often contain large concentrations of natural radioactive elements, contribute to the transfer of radionuclides to water through leaching action [3]. The level of naturally occurring radioactive elements in the marine environment has gradually increased due to human activities, such as mining and processing of ores, production of natural oil and gas, and combustion of fossil fuels in coal-fired power plants. Moreover, anthropogenic contributions are made by underwater nuclear device tests, post-nuclear disposal of industrial and radioactive waste, recycling of spent nuclear fuel, and accidents, including leaks from nuclear power plants [4,5,6,7]. Among the radionuclides, uranium, radium, and radon are soluble in seawater, whereas thorium is almost completely insoluble. These can dissolve in seawater and then attach to sediment on the seabed and suspended plankton in the seawater. These dissolved radionuclides and plankton, in the long run, contaminate marine organisms, including fish, crustaceans, and several types of shellfish [8].
In addition, trace metals are released into the environment from different sources, such as transportation, industrial activities, fossil fuels, agriculture, urbanization, and other human activities [6,7,8,9]. The release of large quantities of trace metals into nature has resulted in several environmental problems due to their non-biodegradability and persistence. Marine life can have a considerable aptitude for bioaccumulation and biosorption of trace metals [10,11,12]. Under certain environmental conditions, such as consolidated effects of biotic and abiotic factors, such as plants, animals, and microbes; the amount of sunlight in the ecosystem; the amount of oxygen and nutrients dissolved in the water; proximity to land; depth; and temperature; these trace metals may accumulate to a toxic concentration and cause ecological damage by threatening the health of aquatic and terrestrial organisms, including humans [1,13,14]. The principal pathway leading to human exposure to radionuclides and trace metals is the consumption of seafood. A large variety of seafood is eaten. Fish and crustacean species, because of their important dual role as both an integral element of marine food webs and a commercial food product for humans, are frequently selected as indicator organisms in studies related to pollution or the bioaccumulation processes of various noxious substances [15]. The bioaccumulation of trace metals and isotopic contaminants by the tissues and organs of marine organisms has been studied globally and has led to the adoption of the bioindicator concept for environmental quality assessments [5,6,7,8,16,17].
Research on the bioaccumulation of radionuclides and trace metals in the most commonly consumed fish and crustacean species in the Bay of Bengal region is limited. Given the importance of such knowledge, this study was carried out to determine the radioactivity and trace metal levels in six marine fish and four crustacean species of the northern coastal belt of the Bay of Bengal, Bangladesh. The bioaccumulation levels of radioactive materials (226Ra, 232Th, 40K, and 137Cs) and trace metals (Cd, Pb, Zn, Cu, Ni, Fe, Mn, and Cr) were estimated by gamma spectrometry and atomic absorption spectrometry, respectively. Different radiological and health-hazard parameters were also calculated to assess the marine environmental quality in this region concerning these pollutants, in addition to the health risks resulting from the consumption of the studied seafood.

2. Materials and Methods

2.1. Sample Collection and Preparation

The studied field locations were openly accessible, and the organisms collected did not belong to any protected species, so permissions were not required at the time of collecting samples from the studied locations. The investigated fish and crustaceans were obtained from four fish/seafood markets of the northern coastal belt of the Bay of Bengal, Chattogram, Bangladesh (Figure 1), during the rainy (July) and autumn (September and October) seasons of 2017. Bangladesh is a country of six seasons (summer, rainy, fall, autumn, winter, and spring). The fish investigated in this study are mostly available in rainy and autumn seasons. Hence, we obtained our samples in these two seasons. The fish samples were obtained from fishing boats operating in the corresponding locations, while the crustacean samples were obtained from a local sea food market in Chattogram city. A total of 20 samples (10 samples from each season) were chosen for investigation. The studied organisms were six fish and four crustacean species, locally available and commercially important, i.e., Tenualosa ilisha (Ilish), Harpodon neherues (Lotia), Sillaginopsis panijus (Sundora Baila), Sardinella longiceps (Colombo), Trichiurus lepturus (Churi), and Konosirus punctatus (Shad) concerning the fish species, and Scylla serrata (mud crab), Portunus sanguinolentus (three-spot swimming crab), Hemigrapsus takanoi (Asian shore crab) and Penaeus semisulcatus (cat tiger shrimp) concerning the crustaceans.
The details of the collected samples are given in Table 1. All the samples were labelled, stored in ice, and, on the same day, transported to the laboratory and washed with clean water and distilled water. Afterwards, the samples were dried in filter paper, packed in polyethylene bags, and stored in a refrigerator of the Laboratory of Atomic Energy Centre, Chattogram, to avoid degradation, spoiling, contamination, or any other decomposition until further treatment and analysis were done. The collected fish and crustacean samples were measured for the total wet weight (WW) and recorded beforehand. For the analysis of radionuclides and trace metal concentrations, all the animals were used in their entirety, since the analysis was intended to quantify the radioactivity and the metal concentrations of each organism.
Each sample was sun-dried at least one week to remove the extra water and subsequently oven-dried at about 70 °C to obtain a constant weight. The samples were then re-weighed to determine the dry/wet ratio. Finally, the dried samples were ground, sieved for homogeneity, and stored in clean and dry uncontaminated empty cylindrical plastic containers of uniform size (2.8 cm × 8.0 cm), sealed with wide vinyl adhesive tapes around their screw necks to air tighten for succeeding uses.
For the trace metal determination, a part of the powdered samples was kept aside in clean and dry sealable plastic bags and kept in an air-tight glass jar to save the samples from any types of chemical reactions. Five grams of the homogenized powder were taken from each specimen and placed in a 250 mL digestion beaker. A digestion mixture containing 6.0 mL of high purity nitric acid (70%) and 2.0 mL of hydrochloric acid (37%) were added and heated at 60 °C for half an hour. After cooling down for 15 min, 4.0 mL of hydrogen peroxide (30%) was added to each beaker and heated until a clear solution was obtained and the volume decreased into half of its original volume following the standard operating procedure described elsewhere [18]. The digested portions were filtered through Whatman filter paper (No. 42) and diluted to a final volume of 50 mL using de-ionized water.

2.2. Methods for Determining Radioactivity and Trace Metals

2.2.1. Gamma Spectrometry Analysis

For the natural and artificial radioactivity measurement of each sample, about 100 g of dried fish/crustacean samples were weighed and placed in individual plastic containers. The samples were kept 4 weeks before the analysis in airtight conditions to allow secular equilibrium between thorium and radium and their short-lived progenies [14].
The activity concentrations of 226Ra, 232Th, 40K, and 137Cs in the samples were determined using an NATS GCD-40 190 p-type HPGe (Baltic Scientific Instruments, Riga, Latvia), gamma-ray spectrometer (63.3 mm crystal diameter, 61.3 mm thickness, +2200 V operating bias voltage). The low background detector of relative efficiency of 43.1% and an energy resolution of 1.74 keV full width at half maximum (FWHM) at the 1332 keV peak of 60Co was enclosed in a cylindrical lead shield. The counting system was connected to an 8 k multi-channel analyzer (MCA 527, GBS Elektronik GmbH) with associated electronics for data acquisition of photo-peak areas. A Spectral Line GP© was used to analyze the gamma-ray counts received from the samples. Energy calibration and the absolute photo-peak efficiency evaluation were performed using standard reference material (Code: 8501-EG-SVE, Eckert and Ziegler Analytics), diluted with a multi-nuclide gamma-ray source (241Am, 109Cd, 57Co, 139Ce, 113Sn, 85Sr, 137Cs, 88Y, 60Co) having homogeneously distributed activity, and maintaining the same geometry and density as the plastic containers containing the samples. To minimize the statistical counting error, the samples were counted for a period of 30,000 s. An empty container was also counted under the same conditions to determine the background counts. To calculate the specific activities, the background counts for the same counting condition were deducted from the counts of each sample to obtain the net counts. For spectrum analyses, the single transition gamma-ray line 1460.822 keV was used to determine the activity concentrations of 40K. The gamma-ray photo-peaks of 295.221 keV and 351.922 keV from 214Pb, and 609.320 keV, 1120.310 keV and 1764.551 keV from 214Bi were used to determine the activity concentrations of 226Ra. The activity concentrations of 232Th were determined using the net counts under the 238.630 keV and 300.087 keV photo-peaks from 212Pb, 911.205 keV and 968.970 keV photo peaks from 228Ac, and 583.190 keV and 2614.533 keV from 208Tl. For the evaluation of 226Ra and 232Th activity, a weighted mean approach was applied using the aforementioned gamma lines [19,20]. Much care was taken to prevent contamination during the investigation.

2.2.2. Determination of Trace Metals

The fish and crustacean samples were analyzed for eight trace elements, Cd, Pb, Zn, Cu, Ni, Fe, Mn, and Cr, using an atomic absorption spectrophotometer (HITACHI Z-2000) [21]. Four standard solutions of different known concentrations were prepared, and the elemental concentrations in the unknown samples were determined by extrapolation from the calibration curve. The digested samples were directly aspirated into the flame (air–acetylene fuel mixture). The concentration corresponding to the absorption in the digest was determined by using the absorption mode. The minimum detection limits of the analyzer for the investigated trace metals in ppm are 0.002 (Cd), 0.010 (Cu), 0.005 (Zn), 0.050 (Pb), 0.010 (Mn), 0.020 (Fe), 0.020 (Ni), and 0.020 (Cr) [22]. The limits of detection (LODs) for all the elements analyzed in the samples were calculated as the blank signal plus three times its standard deviation, whereas the limit of quantification (LOQ) was calculated as ten times the standard deviation of the blank signal, following [23,24]. The recovery estimation was performed by the laboratory during method development and validation processes using standard reference materials (SRMs) and certified reference materials (CRMs). However, this time we used secondary reference material made from commercial standard solutions of each element. At each step of the digestion processes, acid blanks (laboratory blank) were prepared to ensure that the samples and chemicals used were not contaminated. Each set of digestion had its acid blank and was corrected by using its blank. The measurement of each sample was taken three times. All the experimental values were reported as mean value ± standard deviation (SD). Throughout the analysis, all the trace metal standards were prepared and run to check the precision of the instrument. The quality control and quality assurance protocol set by the U.S. Environmental Protection Agency for metal analysis were used. The quality assurance testing relied on the blank controls, and the yield of the chemical procedure was used.

2.3. Radiation Dose and Risk Assessment

2.3.1. Radiation Dose Assessment

Activity Concentration

The activity concentrations (Bq kg−1) of the natural and anthropogenic radionuclides in the measured samples were evaluated by the following equation [25]:
A Bq   kg 1 = CPS × 1000 ε γ I γ M
where CPS = net count per second (i.e., CPS for sample—CPS for background), ε γ = detector efficiency of the specific γ-ray, I γ = intensity of the gamma-ray, and, M = mass of the sample in grams.
The errors of the measurements were expressed in terms of the standard deviation of the ±1σ level.

Total Effective Dose

Annual effective dose is a useful concept that enables the radiation doses from different radionuclides and from different types of sources of radioactivity to be added. Estimation of the radiation-induced health effects associated with the intake of radionuclides in the body is proportional to the total dose delivered by the radionuclides while resident in the various organs. Radiation doses ingested are obtained by measuring radionuclide activity in foodstuffs (Bqkg−1) and multiplying these by the masses of food consumed over a certain period (kgd−1 or kgy−1). A dose conversion factor (SvBq−1) can then be applied to give an estimate of the ingestion dose. Thus, the ingested dose is given by [26,27]:
annual   effective   dose   ( Svy 1 )   =   concentration   ( Bq.kg 1 )   ×   annual   intake ( kgy 1 )   ×   DCF   ( SvBq 1 )
where DCF is the standard dose conversion factor, which is equal to 0.2800 μSvBq−1 for 226Ra, 0.2300 μSvBq−1 for 232Th, and 0.0062 μSvBq−1 for 40K [25].
Therefore, the total effective dose (Svy−1) via ingestion is calculated by the following formula:
total   annual   effective   dose   =   { C R ( R 226 a ) × I F ×   E D } + { C R ( T 232 h ) × I F ×   E D }   + { C R ( K 40 ) × I F ×   E D }  
where CR is the concentration of radionuclides in ingested fish/crustaceans (Bqkg−1), IF is the annual intake (kgy−1) of fish/crustaceans containing radionuclides, and ED is the ingestion dose conversion factor for radionuclides (SvBq−1). The intake rates for Bangladeshi consumers were taken from the “Year Book of Fisheries Statistics of Bangladesh 2018” [28].

2.3.2. Radiation Risk Assessment

Internal Hazard Index (Hin)

Radon and its short-lived descendants are hazardous to the respiratory organs. The internal hazard index (Hin) is used to quantify the internal exposure to radon and its progenies, which is given by the equation:
H in = C Ra 185 +   C Th 259 +   C K 4810
where C Ra , C Th , and C K are the concentration (Bqkg−1) of Ra, Th, and K, respectively.
The values of the internal hazard index (Hin) must be less than unity for the radiation hazard to be negligible [29,30].

Excess Lifetime Cancer Risk (ELCR)

Nowadays, cancer is called a life-threatening disease, and the percentage of this disease increases all over the world, including in Bangladesh, due to various reasons. One of the reasons is the effect of radiation on the biological cells, which contributes to a greater extent to increasing cancer incidence. An effort was made to assess the excess lifetime cancer risk due to the ingestion of marine fish and crustaceans by the procedure proposed by the United States Environmental Protection Agency (USEPA) [31]. The following equation was used to calculate the excess lifetime cancer risk [12,32]:
ELCR = A ir ×   A ls ×   R c
where E L C R , A i r , A l s , and R c are the excess lifetime cancer risk, the annual intake of radionuclide (Bq), the average lifespan 72 yr. (life expectancy of a male and a female are 70.6 years and 73.5 years, respectively in Bangladesh [33]), and the mortality cancer risk coefficient (Bq–1), respectively. The values of mortality cancer risk coefficients are 9.56 × 10−9 Bq–1 for 226Ra, 2.45 × 10−9 Bq–1 for 232Th, and 5.89 × 10−10 Bq–1 for 40K [12,31]. The acceptable ELCR limit is 10−3 for radiological risk in general [32,34].

2.4. Health Risk Assessment of Trace Metals

2.4.1. Estimated Daily Intake (EDI)

EDI is measured by the following equation in (mgkg−1 body weight per day) [35]:
EDI = E F × E D × F IR × C f × C M W AB × AT n × 10 3
where E F is the exposure frequency (365 days per year), E D =72 years is the exposure duration, F IR is the ingestion rate, which is taken as 62.58 g per person per day [28], C f is the conversion factor ( C f = 0.208) to convert fresh weight to dry weight considering 79% of moisture content of the fish fillet [35,36], C M is the metal concentration in fish fillet (mgkg−1 dry weight basis), W AB is the average body weight (60 kg) of Bangladeshi adult people [37], and AT n (equal to E F × E D ) is the average exposure time for non-carcinogens [35].
Several organizations such as WHO, FAO, etc., provided guidelines on the intake of metals by a human. The maximum tolerable daily intake (MTDI), provisional tolerable daily intake (PTDI), and the acceptable daily intake (ADI) are used to describe the safe levels of intake for several toxins, including toxic metals. The EDI of trace metals measures the amount of metal intake by an adult (60 kg) by ingestion of fish or crustaceans per day.

2.4.2. Target Hazard Quotient (THQ)

The THQ is non-carcinogenic risk and is dimensionless. In this study, the non-carcinogenic health risks associated with the consumption of fish and crustacean species were assessed based on the target hazard quotients (THQs), and their calculations were done using the standard assumption for an integrated USEPA risk analysis as follows [38]:
THQ = E F × E D × F IR × C f × C M W AB × AT n × RfD × 10 3
E F , E D , F IR , C f , C M , W AB , and AT n are explained in the earlier section. RfD is the reference dose of individual metal (mg/kg/day) (Table 2). The RfD represents an estimate of the daily exposure to which the human population may be continually exposed over a lifetime without a significant risk of deleterious effects. If the THQ is less than 1, the exposed population is unlikely to experience obvious adverse effects. If the THQ is equal to or higher than 1, then there is a potential health risk [39], and related interventions and protective measurements should be taken.

2.4.3. Hazard Index (HI)

To estimate the overall potential health risk related with more than one metal, THQ of every metal is summed up and referred to as hazard index ( HI ). The HI can be calculated by the sum of the target hazard quotients (THQs) of each metal:
HI   =   THQMn   +   THQFe   +   THQCu   +   THQZn   +   THQPb   +   THQCd   +   THQCr   +   THQNi

2.4.4. Target Cancer Risk

For carcinogens, the target cancer risk (lifetime cancer risk) is estimated as the incremental probability of an individual to develop cancer over a lifetime exposure to that potential carcinogen (i.e., incremental or excess individual lifetime cancer risk) [38]. Acceptable risk levels for carcinogens range from 10−4 (risk of developing cancer over a human lifetime is 1 in 10,000) to 10−6 (risk of developing cancer over a human lifetime is 1 in 1,000,000). The equation used for estimating the target cancer risk, which is dimensionless, is as follows [38]:
TR = E F × E D × F IR × C f × C M × CSF o W AB × AT C × 10 3
Here, CSFo is the oral carcinogenic slope factor (mg/kg BW/day)−1, and ATc (equal to E F × E D ) is the average exposure time for carcinogens. Since Mn, Fe, Cu, and Zn do not cause any carcinogenic effects, their CSFo have yet not been established in USEPA 2012. Thus, TR values for the intake of Pb, Cd, Cr, and Ni were calculated to show the carcinogenic risk using oral carcinogenic slope factors ( CSFo ) of these toxic elements given in Table 2.

3. Results and Discussion

Chattogram is the second largest city in Bangladesh, where the main seaport of Bangladesh is located on the bank of the Karnaphuli river. The Chattogram seaport handles ninety per cent of Bangladesh’s export–import trade and has been used by India, Nepal, and Bhutan to join for transshipment. There are thousands of industries including a textile mill, cement factory, tannery, oil refinery, dichlorodiphenyltrichloroethane (DDT) plant, chemical factory, fertilizer factory, paper mills, power plant, dry-dock, paint factory, rayon mills, etc., situated near to the bank of the Karnaphuli river, among which about 200 are identified as pollution causing units continuously discharging unlawfully a huge amount of pollutants that finally enter into the Bay of Bengal [42]. The wastes coming from the municipal sewage system through many canals of Chattogram city is another potential factor of the pollution problem of this bay. Moreover, illegal and/or accidental discharges of grease, fish oil, bilge, garbage, etc., from the merchant and fishing vessels are causing pollution to the Bay of Bengal [43].

3.1. Radiation Dose and Risk Assessment by Radioactivity Analysis

Marine fish and crustaceans are potential bio-indicators when they accumulate the target radionuclides from surrounding waters [15,44]. Monitoring radionuclide levels in fish and crustaceans is of great importance due to their significant contribution to the natural radiation dose received by human beings consuming them [45].
Among all the fish and crustacean samples, 226Ra was detected only in Harpodon neherues, and its value was 5 ± 2 Bqkg−1. There was no anthropogenic radionuclide (137Cs) in any sample. In all the studied fish samples, mean activity concentrations of 232Th and 40K ranged from 7 ± 1 to 190 ± 10 and 210 ± 50 to 360 ± 40 Bqkg−1, respectively. Similarly, in crustacean samples, the activity concentrations of 232Th and 40K ranged from 5.0 ± 2 to 53 ± 10 and 130 ± 40 to 240 ± 70 Bqkg−1, respectively. Further, note that the activity concentrations of 40K were greater than those of the other radionuclides for all samples (Table 3). Mean values of the activity concentrations of 226Ra, 232Th, and 40K in all the fish and crustacean samples were 5 ± 2, 67 ± 9, and 250 ± 50 Bqkg−1, respectively.
The comparative data of the present study with the previously reported studies of different radioactive elements in fish and crustacean samples of the Bay of Bengal region are reported in Table 4. It was reported in 2012 that the activity concentrations of 226Ra, 232Th, and 40K in sea-fish samples of the Black Sea region of Turkey were 0.06 ± 0.01 to 0.96 ± 0.36, 0.12 ± 0.04 to 1.03 ± 0.15, and 35.04 ± 0.24 to 127.4 ± 2.3 Bqkg−1, respectively [14].
In all these cases, the results were very much lower than those of the present observations. Conversely, the mean activity concentration of 226Ra, 232Th, and 40K in marine fish of the Straits of Malacca was reported as 4.05 ± 0.48 to 7.83 ± 0.78, 1.93 ± 0.24 to 6.21 ± 0.53, and 288 ± 15 to 399 ± 20 Bqkg−1, respectively, in 2015 [12], being considerably comparable to the present study except the concentration of 232Th. The obtained activity concentration of 232Th and 40K in all the fish and crustacean samples were 67 ± 9 and 250 ± 50 Bqkg−1, respectively, which are almost similar to the activity concentration obtained in crab samples from river Odeomi, Ogun State, at the Southwest of Nigeria [46]. It should be noted that if we liken the present study to previous studies for fish [47,48] at the same region, there is a noticeable increment in the levels of different radioactive elements in those marine organisms of the Bay of Bengal region (shown in Table 4). This increment might be due to the wide variety of activities (e.g., housing, tourism, power generation plants, petroleum, steel, shipbuilding, chemical, pharmaceutical, textile, vegetable oil refineries, glass manufacturing industries, etc.) around the Bay of Bengal region, which have been diversified over the years. Multi-functionality and diversification of industries, fisheries, and agriculture are probably linked with these changes in the marine environment. The absence of the anthropogenic radionuclide (137Cs) indicates that there was not any effect on the marine organisms analyzed due to the post-nuclear disposal of industrial and radioactive waste, underwater nuclear device tests, accidents including leaks from nuclear power plants and from recycling of spent nuclear fuel, etc. As Bangladesh is establishing its first nuclear power plant (NPP) at Rooppur, Pabna, this baseline data can help to further monitor these activities.
The ELCR values of 226Ra, 232Th, and 40K in all the fish and crustaceans are shown in Table 3. It is to be mentioned here that ELCR for 226R was below detection level (BDL), except for in Harpodon neherues. However, all of the obtained values (shown in Table 3) were lower than the acceptable ELCR limit of 10−3 for radiological risk in general, indicating no health hazard to the consumers [32,34].
The estimated annual effective doses due to intake of 226Ra, 232Th, and 40K from the consumption of seafood (fish and crabs) from the Bay of Bengal are presented in Table 5, and the comparison between the mean annual effective dose and its world average values are shown in Figure 2. The average annual effective dose for 226Ra and 40K were found to be within UNSCEAR acceptable limits. Though the average annual effective dose for 232Th was found to be three times greater than the UNSCEAR acceptable limits, the total values of annual effective dose were within the acceptable limits, indicating no threat to the consumers. Maximum and minimum values of the total internal hazard index were 0.79 ± 0.06 and 0.04 ± 0.01, respectively, and all the values were less than 1, showing that there is no health hazard to the consumers.

3.2. Pollution Level and Health Risk Assessment of Trace Metal Analysis

3.2.1. Trace Metal Concentration

The intake, bioassimilation, and subsequent bioaccumulation of trace metals in marine organisms are significantly affected by the dissimilar aquatic geochemistry of the trace metals as well as the diverse feeding habits and living methods of fish and crustaceans. Among marine animals, fish are possibly one of the most mobile and capable of travelling long distances. Compared to other types of organisms, fish are also on a high trophic level in the food chain; hence, their diet is probably the most diverse of the species studied here. Crustaceans are benthic organisms that usually live on or near the sea floor and are capable of travelling some distance. They often feed on organic debris but also on small crustaceans and fish on or near the sea floor. Although the trace metal concentrations in the various species of marine fish and crustaceans analyzed in the present study were in a wide range of variations, these aquatic organisms also showed metal accumulation patterns that were noteworthy (see Table 6).
The trace metal concentrations found in individual fish muscles for both rainy and autumn seasons varied for Pb, 7.5–0.12, Cu: 4.3–1.3; Zn, 170–45; Mn, 24–5.5; Cd, 2.1–0.10; Ni, 13–0.28; Cr, 2.6–0.16; and Fe, 1300–11 mgkg−1 dry weight. In addition, in individual crustacean samples of both rainy and autumn seasons, these values varied for Pb, 1.9–0.25; Cu, 100–39; Zn, 150–79; Mn, 640–15; Cd, 3.4–0.09; Ni, 2.3–0.31; Cr, 3.3–0.63; and Fe, 600–9.4 mgkg−1 dry weight.
Most of the chemical elements present in fish and crustaceans are essential for biota at low concentrations; however, high concentrations of these elements can become toxic for them. Similarly, some metals (e.g., Fe, Mn, Ni, Cu, and Zn) are necessary for proper metabolic reactions in the human body, and other elements (e.g., Cd, Cr, and Pb) are of unknown benefits and may become toxic in the cases of chronic exposure to humans [50]. The obtained trace metal concentrations in the ten marine fish and crustacean samples analyzed in the present study in the rainy season followed the order of Fe > Mn > Zn > Cu > Ni > Cr > Cd > Pb and in the autumn season followed the order of Fe > Mn > Zn > Cu >Cr > Pb > Cd > Ni. However, in both seasons, the average concentration followed the order of Fe > Zn > Mn > Cu > Ni > Pb > Cr > Cd in fish samples and of Fe > Mn > Zn > Cu > Cr > Cd > Pb > Ni in crustacean samples. This demonstrated that marine organisms in different groups had different accumulation mechanisms for trace metals. Additionally, the different concentrations of chemical elements in the marine environment and the age of the marine organisms analyzed may also be responsible for these trends.
The maximum allowed concentration of Pb in fish is 0.21 mgkg−1 DW set by JECFA [51], whereas in our study we found that the fish Sillaginopsis panijus, Sardinella longiceps, Trichiurus lepturus, and all the crustacean species contained Pb concentrations exceeding the limit. According to FAO/WHO, the acceptable limit of Cd for human consumption is 0.20 mgkg−1 [52]. Among the fish species, Sardinella longiceps, Konosirus punctatus, and among the crustacean species Portunus sanguinolentus, Scylla serrata, and Penaeus semisulcatus exceeded this limit. In addition, the permissible limit for Cr is 0.20 mgkg−1 DW set by the National Academic Press, Washington DC, 1989 [51], whereas all the studied samples exceeded this limit. These three trace metals (Cd, Cr, and Pb) are considered to be toxic and all of them were in an excessive content in some of the studied marine organisms, demonstrating that the aquatic fauna was contaminated by these metals. Other essential trace elements (e.g., Cu, Zn, Mn, Ni, and Fe) were also observed in excessive contents in some of the studied samples. This indicates the overall pollution status of the studied area due to industrial activities, fossil fuels, agricultural run-off, ship-breaking activities, and other human activities around the coastline.
A comparison between available fish species metal concentration data and the present study is shown in Table 7. It is evident that the range of concentration of metal elements in other parts of the world are lower than that of the presently studied data [52,53,54,55,56]. On the other hand, the obtained data is comparable to similar studies in the neighboring regions [57,58]. If we liken the present study with similar kinds of studies conducted in the Bay of Bengal in the past, the range of metal element concentrations are also comparable [59,60,61], though it indicates a gradual increment in metal accumulation by the fish of those regions. It seems that day by day, the rate of environmental disturbances and the pollution levels increase due to different reasons, such as the establishment of different industries beside the rivers, which is gradually increasing and also affects the marine environment progressively, contaminating the marine organisms in the long run. Presently, it is an issue of concern for the local populations, but in the near future it might be the threat for the surrounding environment of all organisms, including humans.

3.2.2. Estimated Daily Intake (EDI)

EDI and mean EDI values of respective trace elements (Cd, Pb, Zn, Cu, Ni, Fe, Mn, and Cr) for the fish and crustacean samples of both seasons are shown in Table 8. The corresponding Figure 3 shows the comparative results of estimated daily intake (EDI) values with the recommended daily intake (RDI) values. As the values of EDI < RDI for both fish and crustaceans, there is no risk from the consumption of the studied seafood.

3.2.3. Target Hazard Quotients (THQ)

The dimensionless target hazard quotient (THQ) is the indicator of non-carcinogenic health risks associated with the consumption of food (fish and crustaceans). The mean THQ values for the trace metals Pb, Cu, Zn, Mn, Cd, Ni, Cr and Fe are given in Table 8. After analysis of all the fish and crustacean samples, the obtained THQ values for different metals were less than 1, which indicates that the exposed population is unlikely to experience obvious adverse effects [39].

3.2.4. Hazard Index (HI)

The value of the hazard index, which is obtained by the summing of the target hazard quotients of each metal, was used to assess the overall potential health risk posed by more than one metal. The obtained hazard index value was 8.82 × 104, which is less than 1, indicating that there are no health hazards to the local consumers (Table 8).

3.2.5. Target Cancer Risk (TR)

The obtained values of target cancer risk for Pb, Cd, Ni, and Cr due to exposure from the consumption of the targeted six fish and four crustacean species analyzed in the present study are shown in Figure 4. Generally, the values of TR lower than 10−6 are considered as negligible, while those above 10−4 are considered to be unacceptable, and those in between 10−6 and 10−4 are considered as an acceptable range [37]. The present study reveals that only TR for Pb were below the benchmark and those for Cd, Ni, and Cr were above the benchmark, indicating that the fish and the crustaceans were becoming polluted. This also increased the risk of chronic cancer due to exposure of Cd, Ni, and Cr through fish and crustacean consumption.

4. Conclusions

The radioactivity and the trace metal concentrations were studied in certain commercially important marine biota (fish and crustaceans) from the Bay of Bengal, Bangladesh, in two different seasons (rainy and autumn). Through the analysis of different radiological parameters, the present study revealed an elevated activity concentration compared to the acceptable limits in some of the samples. Comparing the present results with the reported results of the different regions of the world, including similar studies at the Bay of Bengal, it can be concluded that the studied region possesses more radioactive elements than the other marine regions. It was also observed that the marine radioactivity of the studied region is gradually increasing, day by day. The results reflect the contribution of technologically enhanced naturally occurring radioactive material (TENORM) pollutants, largely expected to be a result of power generation plants, petroleum, steel, shipbuilding, chemical, pharmaceutical, textile, vegetable oil refineries, glass manufacturing industries, etc. However, the present study indicates that radionuclide intake from the consumption of Bay of Bengal fish and crustaceans still poses an insignificant threat to public health.
Conversely, the metal concentrations of a few trace elements are higher than the acceptable limits in most of the samples, although the studied fish and crustaceans are still safe for human consumption. However, the target cancer risk (TR> 10−4) due to exposure to cadmium, nickel, and chromium indicates that consumer risk persists.
As a whole, the present study indicates an increase in the pollution by radioactivity and trace metals in the marine environment of the Bay of Bengal, mostly derived from an increase in human activities in the region. Therefore, consuming the seafood from the studied region has the potential to cause adverse health impacts if not controlled, and at the same time, all the stakeholders should take proper initiatives to prevent environmental pollution of the Bay of Bengal. The results reported here can be used as baseline data, though the sample size is not large enough. Similar studies should be planned and carried out periodically with higher sample sizes to monitor any changes in marine pollution of the Bay of Bengal in the future. Statistical analyses should also be employed in future studies for better understanding of the scenario.

Author Contributions

Conceptualization, K.P.B., A.K.M.S.I.B., S.H.2 and M.B.H.; methodology, S.H.1, N.D., A.K.M.S.I.B. and S.H.2; software, K.P.B., S.H.1 and N.D.; validation, A.K.M.S.I.B., S.C.G., S.H.2 and M.B.H.; formal analysis, K.P.B., S.H.1 and N.D.; investigation, K.P.B., S.H.1, N.D. and A.K.M.S.I.B.; resources, A.K.M.S.I.B. and S.H.2; data curation, K.P.B., S.H.1 and N.D.; writing—original draft preparation, K.P.B. and A.K.M.S.I.B.; writing—review and editing, S.H.1, A.K.M.S.I.B. and S.C.G.; visualization, A.K.M.S.I.B. and S.H.2; supervision, A.K.M.S.I.B., S.H.2 and M.B.H.; and project administration, S.H.2 and M.B.H. All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Data Availability Statement

The data presented in this study are contained within the article.

Acknowledgments

The authors are thankful to the authority of the Atomic Energy Centre, Chattogram, Bangladesh Atomic Energy Commission, for providing support and technical co-operation to analyze the specimens using the HPGe and AAS. The authors are also delighted to express their gratefulness to the Ministry of Shipping, Peoples’ Republic of Bangladesh, for their utmost support. Thanks also goes to Masuda Begum Sampa, Kyushu Institute of Technology, Japan for the fruitful discussion.

Conflicts of Interest

The authors declare no conflict of interest.

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Figure 1. Location of the study area on the (a) world map, (b) Bangladesh map, and (c) Bay of Bengal map.
Figure 1. Location of the study area on the (a) world map, (b) Bangladesh map, and (c) Bay of Bengal map.
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Figure 2. Comparison between the estimated annual effective doses obtained in the present study with the world average values [32].
Figure 2. Comparison between the estimated annual effective doses obtained in the present study with the world average values [32].
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Figure 3. Comparison between estimated daily intake (EDI) values and recommended daily intake (RDI) values obtained in the present study.
Figure 3. Comparison between estimated daily intake (EDI) values and recommended daily intake (RDI) values obtained in the present study.
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Figure 4. Targeted cancer risk (TR) values for associated toxic element consumption from the studied seafood (fish and crustaceans) at the Bay of Bengal (Bangladesh) during rainy and autumn seasons of 2017.
Figure 4. Targeted cancer risk (TR) values for associated toxic element consumption from the studied seafood (fish and crustaceans) at the Bay of Bengal (Bangladesh) during rainy and autumn seasons of 2017.
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Table 1. Marine organisms collected and analyzed during rainy and autumn seasons of 2017 at the Bay of Bengal (Bangladesh).
Table 1. Marine organisms collected and analyzed during rainy and autumn seasons of 2017 at the Bay of Bengal (Bangladesh).
Sample TypeName of the
Organism/Local Name
Scientific NameRange of the Weight in gmRange of the Length in cmAverage
Moisture
Total
Sample Weight in Kg
Sampling
Location and Season
6 Fish samplesHilsa fish/
Ilish fish
Tenualosa
ilisha
588–62638.0–38.586.01%1.214(L-1) and R
274–30629.0–30.01.204(L-2) and A
Bombay duck/
Lotia fish
Harpodon
neherues
62–10222.0–25.590.22%0.844(L-1) and R
22–8818.0–27.00.890(L-2) and A
Flathead sillago/
Sundara Baila
Sillaginopsis
panijus
82–15025.0–29.083.01%0.960(L-1) and R
168–32030.0–38.01.152(L-1) and A
Indian oil sardine/
Colombo fish
Sardinella
longiceps
50–6818.5–19.577.85%1.014(L-1) and R
42–6218.5–20.51.176(L-1) and A
Belt fish/
Churi Fish
Trichiurus
lepturus
84–10644.0–51.080.88%0.964(L-1) and R
240–32262.0–73.01.116(L-1) and A
Dotted gizzard shad/
Shard fish
Konosirus
punctatus
270–29222.0–25.073.41%0.850(L-1) and R
255–28521.0–23.00.820(L-1) and A
4 Crustacean samplesThree-spot
swimming crab
Portunus sanguinolentus90–140-76.44%1.452(L-3) and R
93–145-1.485(L-3) and A
Mud crabScylla
serrata
60–120-82.82%1.441(L-4) and R
55–120-1.411(L-4) and A
Asian Shore crab/
Chati Kakra
Hemigrapsus
takanoi
8–18-78.00%1.438(L-3) and R
8–20-1.410(L-3) and A
Cat tiger shrimp/
Harina Chingri
Penaeus semisulcatus12–2412.0–14.576.51%0.880(L-1) and R
10–2612.0–16.00.850(L-1) and A
Note: (L-1)—Fishery Ghat, Chattogram; (L-2)—Gohira Fishery Ghat, Anwara, Chattogram; (L-3)—Ganga Bari Fish Market, Chattogram; (L-4)—Hazari Goli Seafood Market, Chattogram; R—rainy season and A—autumn season.
Table 2. Reference dose (RfD) and carcinogenic slope factor (CSFo), oral.
Table 2. Reference dose (RfD) and carcinogenic slope factor (CSFo), oral.
Trace ElementsRfD(Mg/Kg/Day)CSFo(Mg/Kg bw/Day)−1Reference of CSFo
Pb4 × 10−38.5 × 10−3[37]
Cu4 × 10−2-[37]
Zn3 × 10−1-[40]
Mn1.4 × 10−1-[40]
Cd1 × 10−31.5 × 101[41]
Ni2 × 10−21.7 × 100[40]
Cr3 × 10−35 × 10−1[41]
Fe7 × 10−1-[40]
Table 3. Mean activity concentration (Bqkg−1) and excess lifetime cancer risk (ELCR) due to the consumption of natural radionuclide from marine fish and crustacean samples collected at the Bay of Bengal.
Table 3. Mean activity concentration (Bqkg−1) and excess lifetime cancer risk (ELCR) due to the consumption of natural radionuclide from marine fish and crustacean samples collected at the Bay of Bengal.
Sl. No.Types of SamplesName of
Organism/Local Name
Scientific
Name
No. of SamplesMean
Activity for 226Ra
(Bqkg−1)
Mean
Activity
for 232Th
(Bqkg−1)
Mean
Activity
for 40K
(Bqkg−1)
ELCR
for
226Ra
ELCR
for
232Th
ELCR
for
40K
1FishHilsa fish/
Ilish fish
Tenualosa
ilisha
2BDL7 ± 1310 ± 30BDL2.60 × 10−52.96× 10−4
2Bombay duck/
Lotia fish
Harpodon
neherues
25 ± 2170 ± 30270 ± 607.07 × 10−56.87 × 10−42.62× 10−4
3Flathead sillago/
Sundara Baila
Sillaginopsis
panijus
2BDL190 ± 10300 ± 60BDL7.60 × 10−42.86 × 10−4
4Indian oil sardine/
Colombo fish
Sardinella
longiceps
2BDLBDL210 ± 50BDLBDL2.03 × 10−4
5Belt fish/
Churi fish
Trichiurus
lepturus
2BDL11 ± 2360 ± 40BDL4.23 × 10−53.44 × 10−4
6Dotted gizzard shad/
Shard fish
Konosirus
punctatus
2BDLBDL250 ± 70BDLBDL2.37 × 10−4
7CrustaceanThree-spot swimming crabPortunus sanguinolentus2BDLBDL190 ± 30BDLBDL1.79 × 10−6
8Mud crabScylla
serrata
2BDL53 ± 10240 ± 70BDL2.12 × 10−42.32 × 10−4
9Asian shore crab/
Chati Kakra
Hemigrapsus
takanoi
2BDL37 ± 6220 ± 50BDL1.47 × 10−42.08 × 10−4
10Cat tiger shrimp/
Harina Chingri
Penaeus
semisulcatus
2BDL5 ± 2130 ± 40BDL2.01 × 10−51.30 × 10−4
Note: BDL—below detection limit.
Table 4. Comparative data of the radioactivity (Bqkg−1) in marine fish and crustacean samples of the Bay of Bengal, Bangladesh.
Table 4. Comparative data of the radioactivity (Bqkg−1) in marine fish and crustacean samples of the Bay of Bengal, Bangladesh.
Sl.
No.
Study YearNumber of Species226Ra232Th40K137Cs238U228RaReference
Fish
1199515-0.31 ± 0.05
to
1.67 ± 0.48
8.5 ± 1.2
to
57.1 ± 5.3
BDL
to
1.98 ± 0.33
0.31 ± 0.05
to
1.19 ± 0.17
-M. N. Alam [47]
22000150.10 ± 0.03
to
1.66 ± 0.24
-18.1 ± 3.4
to
86.4 ± 6.7
0.19 ± 0.04
to
1.47 ± 0.28
-0.39 ± 0.07
to
1.35 ± 0.19
S. Ghose [48]
320172-8.5 ± 9.6
to
13 ± 17
265 ± 417
to
460 ± 310
-9 ± 19
to
13 ± 14
-M. H. Kabir [49]
420206BDL
to
5 ± 2
BDL
to
190 ± 10
210 ± 50
to
360 ± 40
BDL--Present Study
Crustacean
119955-0.36 ± 0.10
to
0.78 ± 0.23
7.32 ± 0.88
to
16.7 ± 1.8
BDL
to
0.47 ± 0.07
0.11 ± 0.01
to
0.49 ± 0.18
-M. N. Alam [47]
220204BDLBDL
to
53 ± 10
130 ± 40
to
240 ± 70
BDLBDL-Present Study
Note: BDL—below detection limit.
Table 5. Annual effective dose, total effective dose and total internal hazard index for different radionuclides in marine fish and crustacean samples collected at the Bay of Bengal (Bangladesh) during rainy and autumn seasons of 2017.
Table 5. Annual effective dose, total effective dose and total internal hazard index for different radionuclides in marine fish and crustacean samples collected at the Bay of Bengal (Bangladesh) during rainy and autumn seasons of 2017.
Sl.
No.
Types of SamplesName of the
Organism/Local Name
Scientific NameNo. of SamplesAnnual
Effective
Dose
for
226Ra
(Svy−1)
Annual
Effective
Dose
for
232Th
(Svy−1)
Annual
Effective
Dose
for
40K
(Svy−1)
Total Annual Effective Dose
(Svy−1)
Total Internal Hazard Index
(Hin)
1FishHilsa fish/
Ilish fish
Tenualosa
ilisha
2BDL3.39 × 10−54.33 × 10−57.72 × 10−50.09 ± 0.01
2Bombay duck/
Lotia fish
Harpodon
neherues
22.88 × 10−58.96 × 10−43.82 × 10−59.63 × 10−40.74 ± 0.13
3Flathead sillago/
Sundara Baila
Sillaginopsis
panijus
2BDL9.90 × 10−44.18 × 10−51.03 × 10−30.79 ± 0.06
4Indian oil sardine/
Colombo fish
Sardinella
longiceps
2BDLBDL2.97 × 10−52.97 × 10−50.04 ± 0.01
5Belt fish/
Churi Fish
Trichiurus
lepturus
2BDL5.52 × 10−55.03 × 10−51.05 × 10−40.11 ± 0.02
6Dotted gizzard shad/
Shard fish
Konosirus
punctatus
2BDLBDL3.47 × 10−53.47 × 10−50.05 ± 0.02
7CrustaceanThree-spot swimming crabPortunus sanguinolentus2BDLBDL2.62 × 10−52.62 × 10−50.04 ± 0.01
8Mud crabScylla
serrata
2BDL2.76 × 10−43.40 × 10−53.10 × 10−40.25 ± 0.05
9Asian shore crab/
Chati Kakra
Hemigrapsus takanoi2BDL1.92 × 10−43.04 × 10−52.22 × 10−40.19 ± 0.03
10Cat tiger shrimp/
Harina Chingri
Penaeus semisulcatus2BDL2.63 × 10−51.90 × 10−54.52 × 10−50.05 ± 0.02
Table 6. Mean metal concentration (mgkg–1 DW) in different marine fish and crustacean samples collected at the Bay of Bengal (Bangladesh) during rainy and autumn seasons of 2017.
Table 6. Mean metal concentration (mgkg–1 DW) in different marine fish and crustacean samples collected at the Bay of Bengal (Bangladesh) during rainy and autumn seasons of 2017.
Name of the Organism/Local NameHilsa Fish/
Ilish Fish
Bombay Duck/
Lotia Fish
Flathead
Sillago/
Sundara Baila
Indian Oil Sardine/
Colombo Fish
Belt Fish/
Churi Fish
Dotted
Gizzard Shad/
Shard Fish
Three-Spot
Swimming Crab
Mud
Crab
Asian Shore Crab/
Chati Kakra
Cat Tiger Shrimp/
Harina Chingri
Scientific nameTenualosa
ilisha
Harpodon neheruesSillaginopsis panijusSardinella longicepsTrichiurus lepturusKonosirus punctatusPortunus sanguinolentusScylla
serrata
Hemigrapsus takanoiPenaeus semisulcatus
Sample TypeFishCrustacean
No. of samples from
two seasons
2222222222
Mean Pb conc. in
mgkg−1 DW
0.19 ± 0.040.25 ± 0.030.37 ± 0.034.6 ± 0.10.37 ± 0.000.12 ± 0.031.4 ± 0.01.6 ± 0.01.4 ± 0.00.12 ± 0.02
Mean Cu conc. in
mgkg−1 DW
4.1 ± 0.11.7 ± 0.11.6 ± 0.02.6 ± 0.01.9 ± 0.03.4 ± 0.162 ± 20100 ± 075 ± 644 ± 0
Mean Zn conc. in
mgkg−1 DW
73 ± 058 ± 061 ± 0170 ± 063 ± 074 ± 0130 ± 1140 ± 1120 ± 183 ± 1
Mean Mn conc. in
mgkg−1 DW
16 ± 05.8 ± 0.018 ± 020 ± 011 ± 010 ± 089 ± 1610 ± 1450 ± 317 ± 0
Mean Cd conc. in
mgkg−1 DW
0.14 ± 0.000.16 ± 0.000.12 ± 0.000.39 ± 0.000.11 ± 0.002.0 ± 0.03.3 ± 0.00.52 ± 0.040.10 ± 0.001.8 ± 0.0
Mean Ni conc. in
mgkg−1 DW
6.9 ± 0.1BDL0.14 ± 0.070.37 ± 0.041.1 ± 0.12.1 ± 0.01.6 ± 0.01.3 ± 0.1BDL0.16 ± 0.02
Mean Cr conc. in
mgkg−1 DW
0.45 ± 0.001.2 ± 0.21.8 ± 0.10.58 ± 0.000.79 ± 0.000.79 ± 0.002.0 ± 0.22.8 ± 0.01.8 ± 0.10.70 ± 0.00
Mean Fe conc. in
mgkg−1 DW
790 ± 105.5 ± 0.1140 ± 1370 ± 2120 ± 1130 ± 1330 ± 1400 ± 2560 ± 355 ± 0
Note: BDL—below detection limit.
Table 7. Trace metal concentrations (mgkg−1 DW) in various species of fish from different areas of the world.
Table 7. Trace metal concentrations (mgkg−1 DW) in various species of fish from different areas of the world.
Sl.
No.
AreaSpeciesPbCdNiCrCuZnMnFeRef.
1Bay of Bengal, BangladeshFive species: P. carcinus, W. attu, S. sinensis, R. rita, S. sihama, and B. strogylurus--2.70–15.20-0.65–66.0026.00–78.801.89–7.1137.90–182.00[61]
2Bay of Bengal, BangladeshSix species: C. neglecta, C. reba, J. argentus, H. nehereus, S. fhasa, and L. savala1.67–2.580.009–0.176.44–7.58-3.33–4.6918.86–33.895.01–11.1460.55–451.10[59]
3Bay of Bengal, BangladeshNine species: L. calcarifer, P. pangasius, P. indicus, I. megaloptera, R. russelliana, L. stylifetus, R. kanagurta, S. guttatum, P. paradiseus, and O. militaris0.58–4.030.04–0.130.73–6.11-0.46–7.7425.67–119.361.46–9.0237.70–118.91[60]
4Northeast Coast of the Bay of Bengal, IndiaFive species: S. phasa, P. argentus, G. sparsipapillus, L. parsia, and C. sp.12.40–19.960.62–1.202.20–3.69ND–3.8916.22–47.9712.13–44.74--[57]
5Cuddalore Coast, Tamil Nadu, IndiaThree species: R. kanagurta, K. axillaris, and S. longiceps-0.35–0.430.62–0.790.66–0.860.42–0.6120.10–26.20--[56]
6Rio de Janeiro, BrazilEleven species: S. salar, S. brasiliensis, P. saltatrix, M. furnieri, C. leiarchus, C. crysos, P. arenatus, M. cephalus, G. brasiliensis, L. villarii, and P. numida0.04–0.300.001–0.09--1.20–2.902.70–9.300.30–1.701.60–7.50[55]
7Northeast Coast of IndiaNine species: H. nehereus, T. trichiurus, S. laticaudus, D. albida, P. argentius, A. sp., F. niger, H. ilisha, and R. kanagurta-0.01–1.10--0.50–28.203.00–99.100.50–12.0010.40–249.70[58]
8Mersing, MalaysiaTwo species: A. thalassinus, and J. belangeri-2.20–2.34--8.80–12.91120.91–217.374.34–9.67-[52]
9Saudi ArabiaThree species: L. nebulosus, P. major, and S. cantharu0.002–0.0030.001–0.001--0.026–0.0930.037–0.3760.008–0.0360.222–1.016[54]
10Bay of Bengal, BangladeshTen species: L. calcarifer, P. pangasius, P. indicus, I. megaloptera, A. cruciger, P. chinensis, S. phasa, S. guttatum, C. reba, and A. arius0.80–6.260.02–0.471.88–7.561.27–4.66≤ 8.5413.22–74.363.63–17.80-[35]
11Terengganu Coastal Area, MalaysiaTen species: S. leptolepis, D. maraudsi, E. lanceolatus, P. tayenus, Rastrelliger, M. cordyla, N. soldado, P. filamentosus, Bramidae, and S. canaliculatus0.0002–0.0070.0008–0.015--0.0021–0.0120.0488–0.1510.0068–0.0410.3995–0.667[53]
12Northwest Mediterranean SeaSix species: G. melastomus, S. canicula, H. dactylopterus, L. boscii, M. poutassou, and P. blennoides0.00–0.900.00–0.030.02–7.00-0.10–10.6012.10–60.30--[7]
13Northeast Atlantic OceanSix species: G. melastomus, S. canicula, H. dactylopterus, L. boscii, M. poutassou, and P. blennoides0.01–0.260.00–0.040.00–0.53-0.40–5.809.90–40.00--[7]
14Bay of Bengal, BangladeshSix species: T. ilisha, H. neherues, S. panijus, S. longiceps, T. lepturus, and K. punctatus0.12–4.60.11–2BDL–6.90.45–1.81.6–4.158–1705.8–205.5–790PS
Note: BDL—below detection limit; ND—not detected; PS—present study.
Table 8. The values of estimated daily intake (EDI), target hazard quotients (THQ), and hazard index (HI) obtained for the marine fish and crustacean samples collected at the Bay of Bengal (Bangladesh) during rainy and autumn seasons of 2017.
Table 8. The values of estimated daily intake (EDI), target hazard quotients (THQ), and hazard index (HI) obtained for the marine fish and crustacean samples collected at the Bay of Bengal (Bangladesh) during rainy and autumn seasons of 2017.
Sampling TimeRainy SeasonAutumn SeasonMean
EDI
(mgkg−1 Body Weight per Day)
Mean THQHI
Trace MetalsAverage Concentration
(mgkg−1) DW
EDI
(mgkg−1 Body Weight per Day)
THQAverage Concentration
(mgkg−1) DW
EDI
(mgkg−1 Body Weight per Day)
THQ
Pb0.798.29 × 10−44.31 × 10−51.261.31 × 10−38.54 × 10−51.07 × 10−36.43 × 10−5




8.82 × 10−4


Cu27.202.84 × 10−21.48 × 10−433.103.45 × 10−21.80 × 10−43.15 × 10−21.64 × 10−4
Zn93.929.80 × 10−26.79 × 10−598.691.03 × 10−17.14 × 10−51.01 × 10−16.96 × 10−5
Mn122.511.28 × 10−11.90 × 10−4128.201.34 × 10−11.99 × 10−41.31 × 10−11.94 × 10−4
Cd0.848.80 × 10−41.83 × 10−40.889.18 × 10−41.91 × 10−48.99 × 10−41.87 × 10−4
Ni1.932.01 × 10−32.09 × 10−50.788.09 × 10−41.20 × 10−51.41 × 10−31.65 × 10−5
Cr1.291.35 × 10−39.36 × 10−51.261.32 × 10−39.15 × 10−51.34 × 10−39.26 × 10−5
Fe308.623.22 × 10−19.56 × 10−5272.242.84 × 10−19.37 × 10−53.03 × 10−19.47 × 10−5
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Biswas, K.P.; Hossain, S.; Deb, N.; Bhuian, A.K.M.S.I.; Gonçalves, S.C.; Hossain, S.; Hossen, M.B. Assessment of the Levels of Pollution and of Their Risks by Radioactivity and Trace Metals on Marine Edible Fish and Crustaceans at the Bay of Bengal (Chattogram, Bangladesh). Environments 2021, 8, 13. https://0-doi-org.brum.beds.ac.uk/10.3390/environments8020013

AMA Style

Biswas KP, Hossain S, Deb N, Bhuian AKMSI, Gonçalves SC, Hossain S, Hossen MB. Assessment of the Levels of Pollution and of Their Risks by Radioactivity and Trace Metals on Marine Edible Fish and Crustaceans at the Bay of Bengal (Chattogram, Bangladesh). Environments. 2021; 8(2):13. https://0-doi-org.brum.beds.ac.uk/10.3390/environments8020013

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Biswas, Krishna Prasad, Shahadat Hossain, Nipa Deb, A.K.M. Saiful Islam Bhuian, Sílvia C. Gonçalves, Shahadat Hossain, and Mohammad Belal Hossen. 2021. "Assessment of the Levels of Pollution and of Their Risks by Radioactivity and Trace Metals on Marine Edible Fish and Crustaceans at the Bay of Bengal (Chattogram, Bangladesh)" Environments 8, no. 2: 13. https://0-doi-org.brum.beds.ac.uk/10.3390/environments8020013

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