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Article

A Multiple N-Glucosylated Peptide Epitope Efficiently Detecting Antibodies in Multiple Sclerosis

1
Interdepartmental Research Unit of Peptide and Protein Chemistry and Biology, Department of Chemistry “Ugo Schiff”, University of Florence, Via della Lastruccia 13, 50019 Sesto Fiorentino, Italy
2
CNR-IC Istituto di Cristallografia, Via Paolo Gaifami 18, 95126 Catania, Italy
3
UMR 8076 CNRS-BioCIS Team of Chemical Biology and [email protected] Platform of Peptide and Protein Chemistry and Biology, Neuville Campus, CY Cergy Paris Université, 5 mail Gay-Lussac, 95031 Cergy-Pontoise CEDEX, France
4
Department of Clinical and Experimental Biomedical Sciences “Mario Serio” and Careggi University Hospital, University of Florence, Largo Brambilla 3, 50134 Florence, Italy
5
Multiple Sclerosis Clinical Care and Research Centre, Department of Neurosciences, Reproductive Sciences and Odontostomatology, Federico II University, Via Sergio Pansini 5, 80131 Naples, Italy
6
Interdepartmental Research Unit of Peptide and Protein Chemistry and Biology, Department of Neurosciences, Psychology, Drug Research and Child Health–Section of Pharmaceutical Sciences and Nutraceutics, University of Florence, Via Ugo Schiff 6, 50019 Sesto Fiorentino, Italy
*
Author to whom correspondence should be addressed.
Current address: Crelux GmbH, am Klopferspitz 19a, 82152 Martinsried, Germany.
Current address: Isvea srl, Via Basilicata 1/3, Località Fosci, 53036 Poggibonsi, Siena, Italy.
§
Current address: Flamma SpA, Via Bedeschi 22, 24040 Chignolo d’Isola, Bergamo, Italy.
Current address: “Stabilimento Chimico Farmaceutico Militare”, Via Reginaldo Giuliani 201, 50141 Firenze, Italy.
Received: 21 May 2020 / Revised: 7 July 2020 / Accepted: 10 July 2020 / Published: 15 July 2020
(This article belongs to the Special Issue Advances in Multiple Sclerosis Research—Series I)
Diagnostics of Multiple Sclerosis (MS) are essentially based on the gold standard magnetic resonance imaging. Few alternative simple assays are available to follow up disease activity. Considering that the disease can remain elusive for years, identification of antibodies fluctuating in biological fluids as relevant biomarkers of immune response is a challenge. In previous studies, we reported that anti-N-glucosylated (N-Glc) peptide antibodies that can be easily detected in Solid-Phase Enzyme-Linked ImmunoSorbent Assays (SP-ELISA) on MS patients’ sera preferentially recognize hyperglucosylated adhesin of non-typeable Haemophilus Influenzae. Since multivalency can be useful for diagnostic purposes to allow an efficient coating in ELISA, we report herein the development of a collection of Multiple N-glucosylated Peptide Epitopes (N-Glc MEPs) to detect anti-N-Glc antibodies in MS. To this aim, a series of N-Glc peptide antigens to be represented in the N-GlcMEPs were tested in competitive ELISA. We confirmed that the epitope recognized by antibodies shall contain at least 5-mer sequences including the fundamental N-Glc moiety. Using a 4-branched dendrimeric lysine scaffold, we selected the N-Glc MEP 24, carrying the minimal epitope Asn(Glc) anchored to a polyethylene glycol-based spacer (PEG) containing a 19-atoms chain, as an efficient multivalent probe to reveal specific and high affinity anti-N-Glc antibodies in MS. View Full-Text
Keywords: Multiple Sclerosis; antibody detection; ELISA; multivalency; N-glucosylated peptide epitopes Multiple Sclerosis; antibody detection; ELISA; multivalency; N-glucosylated peptide epitopes
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MDPI and ACS Style

Nuti, F.; Fernandez, F.R.; Sabatino, G.; Peroni, E.; Mulinacci, B.; Paolini, I.; Pisa, M.D.; Tiberi, C.; Lolli, F.; Petruzzo, M.; Lanzillo, R.; Morra, V.B.; Rovero, P.; Papini, A.M. A Multiple N-Glucosylated Peptide Epitope Efficiently Detecting Antibodies in Multiple Sclerosis. Brain Sci. 2020, 10, 453. https://0-doi-org.brum.beds.ac.uk/10.3390/brainsci10070453

AMA Style

Nuti F, Fernandez FR, Sabatino G, Peroni E, Mulinacci B, Paolini I, Pisa MD, Tiberi C, Lolli F, Petruzzo M, Lanzillo R, Morra VB, Rovero P, Papini AM. A Multiple N-Glucosylated Peptide Epitope Efficiently Detecting Antibodies in Multiple Sclerosis. Brain Sciences. 2020; 10(7):453. https://0-doi-org.brum.beds.ac.uk/10.3390/brainsci10070453

Chicago/Turabian Style

Nuti, Francesca, Feliciana R. Fernandez, Giuseppina Sabatino, Elisa Peroni, Barbara Mulinacci, Ilaria Paolini, Margherita D. Pisa, Caterina Tiberi, Francesco Lolli, Martina Petruzzo, Roberta Lanzillo, Vincenzo B. Morra, Paolo Rovero, and Anna M. Papini 2020. "A Multiple N-Glucosylated Peptide Epitope Efficiently Detecting Antibodies in Multiple Sclerosis" Brain Sciences 10, no. 7: 453. https://0-doi-org.brum.beds.ac.uk/10.3390/brainsci10070453

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