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A Multiple N-Glucosylated Peptide Epitope Efficiently Detecting Antibodies in Multiple Sclerosis
Article

The Use of Electrochemical Voltammetric Techniques and High-Pressure Liquid Chromatography to Evaluate Conjugation Efficiency of Multiple Sclerosis Peptide-Carrier Conjugates

1
Materials Science Department, University of Patras, 26504 Patras, Greece
2
Institute for Health and Sport, Victoria University, Melbourne, VIC 3030, Australia
3
Newdrug, Patras Science Park, 26500 Patras, Greece
4
Department of Physiology and Pharmacology, Cumming School of Medicine, University of Calgary, Alberta, AB T2N 4N1, Canada
*
Authors to whom correspondence should be addressed.
Received: 30 June 2020 / Revised: 10 August 2020 / Accepted: 17 August 2020 / Published: 21 August 2020
(This article belongs to the Special Issue Advances in Multiple Sclerosis Research—Series I)
Recent studies have shown the ability of electrochemical methods to sense and determine, even at very low concentrations, the presence and quantity of molecules or analytes including pharmaceutical samples. Furthermore, analytical methods, such as high-pressure liquid chromatography (HPLC), can also detect the presence and quantity of peptides at very low concentrations, in a simple, fast, and efficient way, which allows the monitoring of conjugation reactions and its completion. Graphite/SiO2 film electrodes and HPLC methods were previously shown by our group to be efficient to detect drug molecules, such as losartan. We now use these methods to detect the conjugation efficiency of a peptide from the immunogenic region of myelin oligodendrocyte to a carrier, mannan. The HPLC method furthermore confirms the stability of the peptide with time in a simple one pot procedure. Our study provides a general method to monitor, sense and detect the presence of peptides by effectively confirming the conjugation efficiency. Such methods can be used when designing conjugates as potential immunotherapeutics in the treatment of diseases, including multiple sclerosis. View Full-Text
Keywords: mannan; peptide; conjugation; MOG35-55; Graphite/SiO2 electrode; voltammetry; HPLC; multiple sclerosis; immunotherapy; vaccine mannan; peptide; conjugation; MOG35-55; Graphite/SiO2 electrode; voltammetry; HPLC; multiple sclerosis; immunotherapy; vaccine
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MDPI and ACS Style

Deskoulidis, E.; Petrouli, S.; Apostolopoulos, V.; Matsoukas, J.; Topoglidis, E. The Use of Electrochemical Voltammetric Techniques and High-Pressure Liquid Chromatography to Evaluate Conjugation Efficiency of Multiple Sclerosis Peptide-Carrier Conjugates. Brain Sci. 2020, 10, 577. https://0-doi-org.brum.beds.ac.uk/10.3390/brainsci10090577

AMA Style

Deskoulidis E, Petrouli S, Apostolopoulos V, Matsoukas J, Topoglidis E. The Use of Electrochemical Voltammetric Techniques and High-Pressure Liquid Chromatography to Evaluate Conjugation Efficiency of Multiple Sclerosis Peptide-Carrier Conjugates. Brain Sciences. 2020; 10(9):577. https://0-doi-org.brum.beds.ac.uk/10.3390/brainsci10090577

Chicago/Turabian Style

Deskoulidis, Efstathios, Sousana Petrouli, Vasso Apostolopoulos, John Matsoukas, and Emmanuel Topoglidis. 2020. "The Use of Electrochemical Voltammetric Techniques and High-Pressure Liquid Chromatography to Evaluate Conjugation Efficiency of Multiple Sclerosis Peptide-Carrier Conjugates" Brain Sciences 10, no. 9: 577. https://0-doi-org.brum.beds.ac.uk/10.3390/brainsci10090577

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