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Microarrays, Volume 1, Issue 3 (December 2012) – 1 article , Pages 107-124

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Review
Integrated Amplification Microarrays for Infectious Disease Diagnostics
by Darrell P. Chandler, Lexi Bryant, Sara B. Griesemer, Rui Gu, Christopher Knickerbocker, Alexander Kukhtin, Jennifer Parker, Cynthia Zimmerman, Kirsten St. George and Christopher G. Cooney
Microarrays 2012, 1(3), 107-124; https://0-doi-org.brum.beds.ac.uk/10.3390/microarrays1030107 - 09 Nov 2012
Cited by 12 | Viewed by 7982
Abstract
This overview describes microarray-based tests that combine solution-phase amplification chemistry and microarray hybridization within a single microfluidic chamber. The integrated biochemical approach improves microarray workflow for diagnostic applications by reducing the number of steps and minimizing the potential for sample or amplicon cross-contamination. [...] Read more.
This overview describes microarray-based tests that combine solution-phase amplification chemistry and microarray hybridization within a single microfluidic chamber. The integrated biochemical approach improves microarray workflow for diagnostic applications by reducing the number of steps and minimizing the potential for sample or amplicon cross-contamination. Examples described herein illustrate a basic, integrated approach for DNA and RNA genomes, and a simple consumable architecture for incorporating wash steps while retaining an entirely closed system. It is anticipated that integrated microarray biochemistry will provide an opportunity to significantly reduce the complexity and cost of microarray consumables, equipment, and workflow, which in turn will enable a broader spectrum of users to exploit the intrinsic multiplexing power of microarrays for infectious disease diagnostics. Full article
(This article belongs to the Special Issue Feature Papers)
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