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Article

Evaluation of Solid Supports for Slide- and Well-Based Recombinant Antibody Microarrays

1
Department of Immunotechnology, Lund University, Medicon Village, Lund SE-22381, Sweden
2
Clinical Cancer Research using Emerging Advanced Technologies for Health (CREATE), Lund University, Medicon Village, Lund SE-22381, Sweden
*
Author to whom correspondence should be addressed.
Academic Editor: Massimo Negrini
Received: 14 October 2015 / Revised: 2 December 2015 / Accepted: 9 December 2015 / Published: 8 June 2016
(This article belongs to the Special Issue Antibody Microarrays in Clinical Proteomics)
Antibody microarrays have emerged as an important tool within proteomics, enabling multiplexed protein expression profiling in both health and disease. The design and performance of antibody microarrays and how they are processed are dependent on several factors, of which the interplay between the antibodies and the solid surfaces plays a central role. In this study, we have taken on the first comprehensive view and evaluated the overall impact of solid surfaces on the recombinant antibody microarray design. The results clearly demonstrated the importance of the surface-antibody interaction and showed the effect of the solid supports on the printing process, the array format of planar arrays (slide- and well-based), the assay performance (spot features, reproducibility, specificity and sensitivity) and assay processing (degree of automation). In the end, two high-end recombinant antibody microarray technology platforms were designed, based on slide-based (black polymer) and well-based (clear polymer) arrays, paving the way for future large-scale protein expression profiling efforts. View Full-Text
Keywords: antibody microarrays; affinity proteomics; solid support; array-in-well; source plate; microarray scanning antibody microarrays; affinity proteomics; solid support; array-in-well; source plate; microarray scanning
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MDPI and ACS Style

Gerdtsson, A.S.; Dexlin-Mellby, L.; Delfani, P.; Berglund, E.; Borrebaeck, C.A.K.; Wingren, C. Evaluation of Solid Supports for Slide- and Well-Based Recombinant Antibody Microarrays. Microarrays 2016, 5, 16. https://0-doi-org.brum.beds.ac.uk/10.3390/microarrays5020016

AMA Style

Gerdtsson AS, Dexlin-Mellby L, Delfani P, Berglund E, Borrebaeck CAK, Wingren C. Evaluation of Solid Supports for Slide- and Well-Based Recombinant Antibody Microarrays. Microarrays. 2016; 5(2):16. https://0-doi-org.brum.beds.ac.uk/10.3390/microarrays5020016

Chicago/Turabian Style

Gerdtsson, Anna S., Linda Dexlin-Mellby, Payam Delfani, Erica Berglund, Carl A.K. Borrebaeck, and Christer Wingren. 2016. "Evaluation of Solid Supports for Slide- and Well-Based Recombinant Antibody Microarrays" Microarrays 5, no. 2: 16. https://0-doi-org.brum.beds.ac.uk/10.3390/microarrays5020016

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