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Article

Actin Cytoskeleton Regulation by the Yeast NADPH Oxidase Yno1p Impacts Processes Controlled by MAPK Pathways

by
Manuela Weber
1,
Sukanya Basu
2,
Beatriz González
2,
Gregor P. Greslehner
1,
Stefanie Singer
1,
Danusa Haskova
3,
Jiri Hasek
3,
Michael Breitenbach
1,
Campbell W.Gourlay
4,
Paul J. Cullen
2,* and
Mark Rinnerthaler
1,*
1
Department of Biosciences, University of Salzburg, 5020 Salzburg, Austria
2
Department of Biological Sciences, State University of New York at Buffalo, Buffalo, NY 14260-1300, USA
3
Laboratory of Cell Reproduction, Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague 4, Czech Republic
4
Kent Fungal Group, School of Biosciences, University of Kent, Kent CT2 9HY, UK
*
Authors to whom correspondence should be addressed.
Antioxidants 2021, 10(2), 322; https://0-doi-org.brum.beds.ac.uk/10.3390/antiox10020322
Submission received: 31 December 2020 / Revised: 17 February 2021 / Accepted: 18 February 2021 / Published: 22 February 2021
(This article belongs to the Special Issue Roles of NADPH Oxidase in Modulation of Signal Transduction and Cellular Metabolism)
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Abstract

:
Reactive oxygen species (ROS) that exceed the antioxidative capacity of the cell can be harmful and are termed oxidative stress. Increasing evidence suggests that ROS are not exclusively detrimental, but can fulfill important signaling functions. Recently, we have been able to demonstrate that a NADPH oxidase-like enzyme (termed Yno1p) exists in the single-celled organism Saccharomyces cerevisiae. This enzyme resides in the peripheral and perinuclear endoplasmic reticulum and functions in close proximity to the plasma membrane. Its product, hydrogen peroxide, which is also produced by the action of the superoxide dismutase, Sod1p, influences signaling of key regulatory proteins Ras2p and Yck1p/2p. In the present work, we demonstrate that Yno1p-derived H2O2 regulates outputs controlled by three MAP kinase pathways that can share components: the filamentous growth (filamentous growth MAPK (fMAPK)), pheromone response, and osmotic stress response (hyperosmolarity glycerol response, HOG) pathways. A key structural component and regulator in this process is the actin cytoskeleton. The nucleation and stabilization of actin are regulated by Yno1p. Cells lacking YNO1 showed reduced invasive growth, which could be reversed by stimulation of actin nucleation. Additionally, under osmotic stress, the vacuoles of a ∆yno1 strain show an enhanced fragmentation. During pheromone response induced by the addition of alpha-factor, Yno1p is responsible for a burst of ROS. Collectively, these results broaden the roles of ROS to encompass microbial differentiation responses and stress responses controlled by MAPK pathways.

Graphical Abstract

1. Introduction

NADPH oxidases are a class of enzymes that consist of six to seven transmembrane helices that coordinate two non-identical heme b molecules via four highly conserved histidines. These proteins catalyze the transfer of electrons from NADPH to FADH, from FADH to the two heme b groups and finally to O2 leading to the production of superoxide (O2−) [1]. The first NADPH oxidase was identified in humans because a lack of this enzyme’s activity is the main driver of chronic granulomatous disease that leads to death during early childhood, which has been attributed to a multitude of infections. Leukocytes of these patients have a defect in the enzyme Nox2 and, thus, lack the ability to perform a respiratory burst that is an important parameter of the innate immune defense [2,3,4]. Therefore, it was assumed for some time that NADPH oxidases are restricted to multicellular organisms. Contradicting this assumption, we were able to demonstrate that a NADPH oxidase-like enzyme (Yno1p) exists in the single-celled yeast Saccharomyces cerevisiae [5]. Confirming our result, homologous NADPH oxidases have been identified in other mono-cellular ascomycetes [6] and even bacteria [7].
In S. cerevisiae, the reactive oxygen species (ROS) produced by Yno1p primarily act as signaling molecules [5]. Although superoxide is directly produced, the main bioactive molecule is hydrogen peroxide [8]. The Yno1p-derived ROS are instantaneously dismutated to H2O2 by Sod1p [8]. In this way, a ROS producing and an antioxidative enzyme are in a strict dependence of each other to fulfil their function. Due to its localization in the peripheral endoplasmic reticulum (ER), Yno1p engages in signaling with proteins in the plasma membrane. The plasma membrane is an important mediator between the exterior and interior of the cell and is thus an important signaling platform for the cell. A multitude of signaling proteins function at the plasma membrane that include receptors, G-proteins, and protein and lipid kinases. A typical example are the yeast casein kinases, Yck1p and Yck2p, which are tethered to the cytoplasmic side of the plasma membrane by the lipid modification, palmitoylation [9]. Both kinases are involved in sensing extracellular glucose and control mediating its import, but are also involved in bud morphogenesis, pheromone signaling, cytokinesis and endocytosis [10,11,12]. It was shown that hydrogen peroxide derived from the Yno1p–Sod1p interaction stabilizes Yck1p in the plasma membrane. As a direct consequence, mitochondrial respiration is downregulated, and at the same time, glycolysis is promoted [8]. Another prominent example is the evolutionary highly conserved monomeric G-protein Ras2p [13]. Similar to Yck1p/Yck2p, Ras2p is involved in extracellular glucose sensing and can function in modulating mitochondrial respiration [14]. Defects in the mitochondrial electron transport chain can induce translocation of Ras2p from the plasma membrane to the mitochondria to mediate a burst of ROS production by Yno1p [15]. Localized ROS in proximity to the plasma membrane can modulate diverse processes ranging from apoptosis to F-actin polymerization [5]. The pathways that lead to the latter process are the focus of the present manuscript.
Many fungal species are capable of undergoing a nutrient-dependent growth response commonly referred to as filamentous growth. Filamentous occurs in many fungal species, and in fungal pathogens can be required for virulence [16]. In the major human fungal pathogen Candida albicans, the NADPH oxidase Fre8p resides in the plasma membrane, particularly at the growing tip of the cell. As in S. cerevisiae, the NADPH oxidase fulfills its function in concert with a superoxide dismutase. Unlike in baker´s yeast, the resulting hydrogen peroxide acts in an extracellular manner. The resulting ROS burst is important for the transition from yeast to hyphal growth and differentiation to the infectious form of C. albicans. In a mouse model of candidiasis, ∆fre8/∆fre8 strains showed significantly shortened hyphae [6].
In contrast to C. albicans, S. cerevisiae is not capable of forming true hyphae, but rather forms pseudohyphae, where cells fully separate by cytokinesis [17,18]. During pseudohyphal growth, cells become elongated due to an extended period of apical growth due to a delay in the cell cycle, which involves vesicle trafficking along a highly polarized actin-based cytoskeleton [19,20]. Cells also reorganize their polarity to grow outward from their ends at the distal poles [21]. In addition, cells remain attached to each other (and can attach to surfaces) due to contacts mediated by cell adhesion molecules. One tractable phenotype in S. cerevisiae and other fungal species is invasive growth, where filamentous cells penetrate into surfaces [22]. The route to filamentous growth in S. cerevisiae is complex and integrates signals from different pathways including the Ras2p-cAMP-protein kinase A (PKA) pathway [18,23,24,25], the Rim101 pathway [26], the Snf1p Nrg1p/Nrg2p pathway and a mitogen activated protein (MAP) kinase pathway commonly referred to as the filamentous growth or fMAPK (filamentous growth MAPK) pathway (Figure 1, [27]). The fMAPK pathway is regulated by a cell-surface glycoprotein, Msb2p, which controls the activity of the Rho GTPase Cdc42p [28]. Like Ras2p, Cdc42p is a monomeric GTPase of the RAS superfamily. When activated by GTP binding, Cdc42p interacts with the serine/threonine p21 activated kinase (PAK) Ste20p, which controls a MAP kinase cascade consisting of Ste11p (MAPKKK), Ste7p (MAPKK), and Kss1p (MAP kinase) (Figure 1) [29]. Kss1p phosphorylates and activates the transcription factors Ste12p and Tec1p, which bind to so called “Filamentation and invasive Response Elements”. These consensus sequences are found in the promoter regions of several genes that are necessary for filamentous growth [27,30]. Many of the pathways that regulate filamentous growth can influence each other’s activities. For example, Ras2p regulates the activity of the fMAPK pathway [31].
The MAP kinase pathway that regulates filamentous growth is composed of proteins that can also function in other MAP kinase pathways in the same cell (Figure 1). Many of the proteins, including Cdc42p, Ste20p, Ste11p, and Ste7p also regulate the mating or pheromone response pathway [32]. The mating pathway is required for haploid cells of complementary cell types to form diploids. During mating, cells express peptide pheromones (a- and alpha-factor) that are recognized by G-protein coupled receptors (GPCR, Ste2p, and Ste3p) on complementary cell types. Detection of pheromone by receptor leads to transcriptional induction of target genes, cell cycle arrest, and morphogenetic changes that prepare the cell to interact with its mating partner. Fusion of the two cell types leads to the formation of a diploid cell. One response of yeast cells that come into contact with pheromones is a morphological change known as shmoo. The formation of shmoos is mainly driven by the actin cytoskeleton: actin patches are concentrated in the shmoo tip, whereas actin cables can be found alongside the axis of polarization [33]. This is a further connecting link to filamentous growth in which primarily actin patches play an important part and are mostly polarized to the distal tip of the outwardly growing cell [20]. It has previously been shown that addition of non-physiological concentrations of alpha factor induce a burst of ROS production after 1.5 h accompanied with apoptotic cell death after 3.5 h. Under native conditions (mixing a with alpha cells) this phenomenon was observed at reduced levels (~30% of all cells) [34].
In addition to mating and filamentous growth, Cdc42p, Ste20p, and Ste11p proteins also regulate one of two redundant branches of the p38-type hyperosmolarity glycerol response (HOG) pathway, which mediates the tolerance to osmotic stress (Figure 1) [35]. In the HOG pathway, two mucin-type glycoproteins function as sensors, one being Msb2p, which also functions in the fMAPK pathway and the other, Hk1p, which does not [36,37,38,39]. These proteins along with the adaptor Sho1p regulate Cdc42p, Ste20p, and Ste11p, which in turn regulates the MAPKK Pbs2p to control the activity of the MAP kinase Hog1p. Hog1p regulates at least five transcription factors: Msn2p, Msn4p, Hot1p, Sko1, and Smp1p [40]. A potential target of Msn2p is YNO1, which suggests that Yno1p might play a role in the osmotic stress response [41]. Therefore, signaling pathways can share components yet induce different responses, which can be critical for the response to stress and cell differentiation. Among the responses to osmotic stress are fragmentation of the vacuole and disruption of the actin cytoskeleton (for a detailed review see [40]). Vacuolar morphology is highly dynamic, and the underlying fission and fusion events are dependent on actin polymerization, which is also mediated by Cdc42p [42]. During vegetative growth, two to three vacuoles exist in yeast cells at the same time. Hypotonic conditions initiate vacuole fusion leading to a large vacuole, whereas salt stress initiates vacuole fission resulting in vacuole fragmentation [43].
In the present study, we explore how Yno1p might impact signaling functions during osmotic stress, pheromone response, and filamentous/invasive growth. We show that Yno1p impacts aspects of these processes in distinct ways. One way in which Yno1p might impact these processes is by its ability to regulate the actin cytoskeleton. Because morphogenetic processes and signaling events require a proper functioning actin cytoskeleton, Yno1p may integrate ROS signals by this mechanism. Therefore, ROS generation may be an important part of the cellular response to diverse stimuli.

2. Material and Methods

2.1. Strains and Media

All strains used in the study are either based on S. cerevisiae strains BY4741 (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0), BY4742 (MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0) [44], or Σ1278b (MATα; ura3-52; trp1∆::hisG; leu2∆::hisG; his3∆::hisG) [45,46]. For details on strains used in this study, their construction, as well as media composition in which the strains were grown, see Supplementary Material and Methods.

2.2. Cloning Experiments

The expression vector p416GPD was used in the study. The LAS17 open reading frame was amplified by PCR from genomic yeast DNA by using Phusion® High-Fidelity DNA Polymerase (NEB, Ipswich, MA, USA) using the following primers: LAS17 forward (5′-CGG GAT CCA TGG GAC TCC TAA ACT C-3′) and LAS17 reverse (5′-CCG CTC GAG TCA CTT ATC GTC ATC CTT GTA ATC CCA ATC ACC ATT GT-3′). The resulting PCR product was cloned into the vector using BamHI and XhoI (NEB, Ipswich, MA, USA). Successful cloning was confirmed by sequencing at Eurofins-MWG-OPERON.

2.3. DHE Measurements

The dihydroethidium (DHE) assay was performed as described in [47]. The yeast strains were grown for 16h in liquid SC−glucose. Cells were collected by centrifugation (5000 rpm 2′) and washed twice with 1 × PBS (phosphate buffered saline). Approximately 5 × 106 cells were resuspended in 180 µL 1 × PBS containing 5 µg/mL DHE (Sigma-Aldrich/Merck, Darmstadt, Germany) and incubated for 15 min in the dark. DHE fluorescence was determined using an Anthos Zenyth 3100 plate reader (excitation: 485 nm; emission: 595 nm). Cell numbers were determined with an electric field multichannel cell counting system (CASYTM; Schärfe; Germany).

2.4. Reporter Measurements

Cells were washed twice in 1 × PBS and 5 × 106 cells were resuspended in 200 µL 1 × PBS. GFP (green fluorescence protein) fluorescence was determined using an Anthos Zenyth 3100 plate reader (excitation: 485 nm; emission: 535 nm) as described [48]. The autofluorescence (cells without GFP expression) was subtracted from the signal reported. For experiments with wiskostatin (racemate) (Calbiochem/Sigma-Aldrich, Merck, Darmstadt, Germany), cells were grown for 18 h in liquid SC (synthetic complete) medium; 200 µM wiskostatin was added for 3–4 h. For experiments with isoamyl alcohol (IAA), cells were diluted to an OD600 (optical density at a wavelenght of 600 nm)= 0.1 in liquid YEPD (yeast extract peptone dextrose) medium. Moreover, IAA (0.5%) was added, and cells were grown for 36–48 h. To evaluate osmotic stress, cells were diluted to an OD600 = 0.1 in liquid YEPD medium. A total of 1 M NaCl was added and cells were grown for 36–48 h. To evaluate filamentous growth, a preculture was grown in SC-raffinose (3%). Cells were diluted to an OD600 = 0.1 in SC-galactose (3%) and grown for~36 h.

2.5. Spot Test Assay

Yeast strains (control; single and double gene deletions) were grown in liquid SC-glucose medium at 24 °C until stationary phase. Cells were diluted to OD600 values of 3.0, 1.0, 0.3, and 0.1. SC-glucose semi-solid agar medium was prepared containing wiskostatin in a range from 10 to 20 μM. To semi-solid agar media, 10 µL aliquots of the diluted yeast strains were spotted. Plates were incubated for 3–4 days at 24 °C.

2.6. Assays to Evaluate Filamentous/Invasive Growth

The plate-washing assay was performed as described [22]. The single-cell invasive growth assay was performed as described [49]. Differential interference contrast (DIC) microscopy was performed with an Axioplan 2 fluorescent microscope (Zeiss, Oberkochen, Germany) with a Plan-Apochromat 100X/1.4 (oil) objective (N.A. 1.4) (cover slip 0.17) (Zeiss, Oberkochen, Germany). Cells were examined at multiple focal planes with an Axiocam MRm camera (Zeiss, Oberkochen, Germany) and AxioVision 4.4 software (Zeiss, Oberkochen, Germany). Adjustments to contrast and brightness were performed using Adobe Photoshop (Adobe, San Jose, CA, USA). ImageJ analysis (http://rsb.info.nih.gov/ij/) was used to quantitate invasive growth [50]. Background intensity was determined for each spot and subtracted from the densitometry of the area of invaded cells. Invasive growth assays were performed in biological replicates (N > 3). The variation in between samples was typically less than 15% across trials, as assessed by independent quantitation methods, ImageJ and OpenLab Software.

2.7. Immunoblot Analysis

Immunoblots were performed as described [51]. Proteins were prepared from cell extracts from cells grown under the indicated conditions and analyzed by sodium-dodecyl-sulfate (SDS) polyacrylamide gel electrophoresis (PAGE), using 10% acrylamide in the running gels. Proteins were transferred from gels to nitrocellulose membranes that were probed with the indicated antibodies. Antibodies that recognize GFP (#11814460001, clones 7.1 and 13.1, Roche) were used at 1:1000 dilution. Antibodies that recognize Pgk1p (Life Technologies; Camarillo, CA, USA; Cat #459250) were used at 1:10,000 dilution. Secondary antibodies goat anti-mouse IgG–HRP (Bio-Rad Laboratories, Hercules, CA, USA; Cat #170-6516) were used for detection of primary antibodies. Band intensity was measured by Image Lab Software (Bio-Rad, Hercules, USA) using unsaturated exposures. Background subtraction was performed based on the software user guidelines. Band intensities were compared to total protein levels based on Pgk1p levels.

2.8. Osmotic Stress and FM4-64 Staining

FM4-64 staining with small modifications was performed as described [52]. Yeast cells were diluted to an OD600 = 0.1 and were grown for 12 h in liquid SC-medium. An aliquot (2 mL) was washed once in YEPD and resuspended in 100 µL YEPD supplemented with 20 µM FM4-64. After a 20 min incubation at 28 °C, cells were wash with SC-medium and incubated for 1 h in 100 µL liquid SC medium. Moreover, 50 µL of this cell suspension was used as a control, 50 µL was supplemented with 1 M NaCl and incubated for >30 min. Cells stained by FM4-64 were analyzed with a × 100 objective (NA = 1.4) using either a Carl Zeiss AG Axioscope (Oberkochen, Germany) or a Nikon (Tokyo, Japan) Eclipse Ni-U equipped with a DS-Fi2 digital camera.

2.9. Pheromone Response Assay

Cells were grown to late exponential phase in liquid SC medium. Shmooing and apoptotic cell death was induced by the addition of 100 µg/mL alpha-factor (GenScript, Piscataway, NJ, USA). After incubation for 4 h at 28 °C under constant shaking, the appearance of shmoos was evaluated by light microscopy. Cell survival was calculated by plating 500 cells on YEPD medium and counting the number of colonies after 3 days of incubation at 28 °C.

2.10. Actin Morphology

Cells were cultivated in 3 mL of SC-Ura media in 6-well plates. The cell suspension was then mixed with fresh SC-Ura medium (1:1) and galactose up to 3% was added after 1 h precultivation. The cells were inspected in the cultivation medium, mounted on a glass slide, covered with a coverslip, and directly imaged at room temperature. Only the first three snapshots of each sample were used for analysis. The distribution of TagRFP-T was analyzed with a 100x Plan Apochromat objective (NA = 1.4). An Olympus IX-81 inverted microscope (Tokyo, Japan) equipped with a Hamamatsu Orca/ER digital camera and the Olympus Cell RTM detection and analyzing system was used. TagRFP-T fusion was detected using RFP filter block (U-MWIY2, excitation; 545–580 nm; emission 610 nm). Images were processed and merged using Olympus Cell RTM (xcellence RT) and Adobe CS6 software. Images presented as Z-stack are maximal projections of stacks obtained using a 0.4-μm steps. Images presented as T-stacks are minimum intensity projection of fluorescence from 15 captured frames. Deconvolution of images obtained by Z-sectioning was done by Wiener filter (WF). Time lapse settings: The image acquisition parameters of T-stack in Figure 9 were one frame per 5 s (fifteen frames in total), and in supplementary movies, one frame per 5s (fifty frames in total).

2.11. Statistical Analysis

Data are presented as arithmetic means ± S.D. We used a one-way ANOVA test including Tukey HSD (honestly significant difference) and a student’s t-test for statistical analysis. Results with a p < 0.05 were considered to be statistically significant.

2.12. Gene Accession Numbers

YNO1: S000003128; HOT1: S000004783; KSS1: S000003272; RAS2: S000005042; ARP2: S000002187; ARP3: S000003826; ABP140: S000005765; CDC42: S000004219; PBS2: S000003664; STE7: S000002318; BEM2: S000000957; TEC1: S000000287; RIM101: S000001019; STE11: S000004354; SMP1: S000000386; STE12: S000001126; SNF1: S000002885; LAS17: S000005707; NRG2: S000000270; NRG1: S000002450; MSN2: S000004640; SOD1: S000003865; HOG1: S000004103; MSB2: S000003246; VRP1: S000004329; YCK1: S000001177; YCK2: S000005098; STE20: S000000999; SKO1: S000005111

3. Results

3.1. Establishment of an YNO1 Expression Reporter

In a previous study [5], we have shown that the ROS produced by the NADPH oxidase Yno1p at physiological levels are not harmful for the cell, but act primarily as signaling molecules. Moreover, overproduction of the Yno1p protein, which induces a burst of ROS, resulted in the induction of apoptotic cell death [5]. To gain further insight into the pathways that may be regulated by Yno1p, an expression reporter was constructed. The YNO1 ORF was replaced by GFP; thus, placing the fluorescent protein under control of the native YNO1 promoter (PYNO1 ∆yno1::GFP-HIS3). The necessary genetic manipulations are summarized in the Supplementary Material and Methods section, and the resulting genetic alterations on chromosome VII are shown in Supplementary Figure S1.
The YNO1 expression reporter was tested under three well-defined settings. One setting was cells lacking mtDNA. It is well established that a rho0 petite yeast strain (a strain completely devoid of mtDNA) shows reduced ROS production [53,54,55]. By using a dihydroethidium (DHE) assay, ROS levels were compared between the rho0 strain and the corresponding wild type strain. DHE was initially used as a superoxide specific probe, but recently it was shown that this dye can be oxidized by hydrogen peroxide in the presence of heme proteins [56]. By this test, the rho0 strain showed reduced levels of ROS production (Figure 2A). The DHE fluorescence signal in the petite strain was more than 80% decreased. Currently, it is assumed that complex III of the respiratory chain is responsible for 80% of the cellular superoxide levels in yeast cells [54]. Surprisingly, the ROS levels in petite strains correlated well with the expression of Yno1p. The wild type strain showed a 5-fold higher expression of PYNO1 ∆yno1::GFP-HIS3 than the non-respiring strain (Figure 2B). This result indicates that cross talk occurs between the mitochondria and the expression of the gene encoding NADPH oxidase. This phenomenon of ROS-induced ROS has been described in other model systems [57,58]. Previously, we and others have demonstrated that Yno1p contributes to~20% of the cellular superoxide levels under normal growth conditions [5,8,15,59]. As shown below, an increase in Yno1p expression accompanied by an increase in ROS production is needed under well-defined cellular settings.
The second condition was in cells experiencing ER stress. It has been established that Yno1p protein levels are controlled by the endoplasmic reticulum-associated degradation (ERAD) pathway. After a diauxic shift, Yno1p levels were absent at the ER [15]. By the Yno1p reporter system, we found that the expression of YNO1 was quite stable during exponential growth and leads to a burst of expression in early stationary phase (~70 h of growth). After 100 h, the GFP signal significantly decreases (Figure 2C). This discrepancy to the data presented in Leadsham at al. [15] could be explained by the fact that the Yno1p itself may be specifically turned over by the ERAD system, whereas GFP is quite stable. GFP may therefore be a useful reporter for switch-on (inducing), but not switch-off (repressing) conditions [60].
The third condition was experiments interrogating the actin cytoskeleton. We have previously shown that a ∆yno1 strain is sensitive to the addition of wiskostatin [5]. Wiskostatin was shown to interact with the evolutionary highly conserved protein N-WASp (isolated from Xenopus laevis eggs) thus interfering with F-actin polymerization [61]. Treating cells harboring the Yno1p reporter with wiskostatin led to an increase in GFP signal. Several wiskostatin concentrations were tested in a range from 50–300 µm wiskostatin and a peak was reached at a concentration of 150–200 µM with a >60% increase in GFP fluorescence. In a petite strain, the difference was even more striking, which showed an~8-fold increase in the expression of the PYNO1 ∆yno1::GFP-HIS3 construct (Figure 2D). Under the described conditions, addition of either 0.5 or 1 mM H2O2 reduced the expression of GFP to the same levels as seen in control cells (Figure 2E).
To define how Yno1p might impact polymerization of F-actin, a spot test was performed. Serial dilutions of yeast cells were spotted on SC plates containing 20 µM wiskostatin and incubated for 3–4 days at 24 °C, which was permissive for growth for the temperature-sensitive strains tested. Under the conditions examined, the ∆las17 and ∆vrp1 strains showed slow growth at 28 °C, and the ∆las17 ∆vrp1 double deletion strain was not viable. Confirming previous results, deletion of YNO1 also led to sensitivity to wiskostatin. The yeast homologue of N-WASp is Las17p, which together with the Arp2/3 complex, promotes the nucleation of branched actin filaments [59]. Surprisingly, deletion of the proposed target of wiskostatin, Las17p, made the cells hypersensitive to the addition of 20 µM of wiskostatin (Figure 3). Therefore, it can be excluded that the N-WASp inhibitor solely binds to Las17p in yeast cells and may indicate that there is at least one other interacting partner. A likely candidate for the binding of wiskostatin is Vrp1p (verprolin; very proline-rich). Vrp1p binds to the N-terminal region of Las17p, which is also rich in proline, and together the two proteins activate the Arp2p/3p complex [62]. Both Vrp1p and Las17p promote the nucleation of F-actin independently of the Arp2/3 complex [63]. In fact, wiskostatin had only a modest effect on growth of the ∆vrp1 strain. A ∆yno1 ∆vrp1 double deletion strongly amplified the wiskostatin sensitivity compared to the corresponding single deletions. By reducing the wiskostatin concentration, the ∆las17 showed growth compared to the ∆las17 ∆yno1 double deletion strain.
Directly upstream in the pathway that regulates Las17p is the Rho GTPase Cdc42p. This GTPase is essential, therefore a ∆bem2 strain was analyzed. Bem2p is the GAP of Cdc42p and upon its deletion the activity of Cdc42p is disturbed. A double deletion strain of BEM2 and YNO1 was hyper-sensitive to wiskostatin and showed a slower growth on plates containing wiskostatin than the appropriate single gene deletions. Yno1p itself is not capable of producing the signaling molecule hydrogen peroxide and is therefore most probably dependent on the superoxide dismutase Sod1p [8]. This assumption was tested by using a ∆yno1 ∆sod1 double deletion strain that showed a high sensitivity to wiskostatin. If any, the phenotype of a SOD1 single gene deletion was modest. A further downstream target of Cdc42p is the PAK Ste20p that mediates osmotic stress response [64], pheromone response [65] and filamentous growth [27]. These three pathways will be studied below.

3.2. Yno1p and the Osmotic Stress Response

It has been reported that salt stress interferes with the cell cycle, leading to growth arrest and ultimately the apoptosis of yeast cells [66]. In subsequent studies, it was shown that in this case programmed cell death is not accompanied by increased ROS levels. Such elevated ROS levels were only observed in specific deletion mutants (e.g., a Δsro7 strain) [67]. Our analysis confirmed this finding. Addition of 1 M NaCl did not lead to a significant increase in superoxide levels in the BY4741 background (Figure 4A). In contrast to the situation in the wild type strain, the absence of mitochondrial DNA and, thus, mitochondrial respiration led to a high response to the addition of NaCl.
The addition of 1 M NaCl increased the amount of ROS in the strain BY4741 rho0 by >17-fold. Under these conditions, the amount of ROS in the petite strain surpassed superoxide levels in untreated wild type cells. The increase of ROS upon NaCl treatment was largely dependent on the yeast NADPH oxidase. Deletion of YNO1 in the petite background led to a strong reduction in ROS levels, although a non-statistical significant increase was still observed. These findings were also mimicked by the YNO1 reporter expression: no increase in PYNO1 ∆yno1::GFP-HIS3 expression was observed in the rho+ background, whereas a 3-fold expression was found in the rho0 background upon addition of 1 M NaCl (Figure 4B). In a petite strain also lower concentrations of NaCl (0.5 mM and 0.7 mM) induced an expression of Yno1p albeit on reduced levels.
We next sought to test whether the osmotic stress induced Yno1p expression is dependent on the Cdc42p/Ste20p proteins. Cdc42p and its effector Ste20p regulate one branch of the HOG pathway (Figure 1) [40,64]. Interestingly, in cells lacking Ste20p, the increase in GFP-signal by NaCl did not occur. Another protein that is important in coping with osmotic stress is Msn2p. This is a cytosol-localized transcription factor that upon stress induction translocates to the nucleus and mediates the expression of stress response genes [68,69]. It is predicted that this transcription factor also binds to the promoter region of YNO1 [41,70]. During osmotic stress, Msn2p migrates to the nucleus (after~8 min) shuttles back to the cytosol presumably once cellular adaptation occurs (after 15 min) [71]. Deletion of MSN2 also abolished the increase in YNO1 expression during salt stress (Figure 4B). Another predictor for the change in osmolarity is vacuolar morphology. In the BY4741 background, these changes in vacuolar morphology are transient [52]. To assay mitochondrial morphology BY4741 and the BY4741 ∆yno1 mutant, cells were prestained with the dye, FM4-64 [72]. FM4-64 is a lipophilic dye that intercalates into the plasma membrane, and after internalization by endocytic events, stains the vacuolar membrane and other internal compartments. In most untreated control cells (Figure 4C), three distinct vacuoles were observed. The addition of 0.4 M NaCl induced fragmentation of vacuoles after 10 min. By 20 min and until cells adapted to stress, the fragmented vacuoles underwent fusion. In cells lacking YNO1, untreated cells showed a higher fragmentation of vacuoles, although on a very subtle level. Similar to wild type cells, addition of NaCl resulted in a complete fragmentation of vacuoles. After 30 min of incubation, no sign of adaptation was observed in the ∆yno1 background, and it took another 30 min until the vacuoles started to revert back to the normal phenotype.

3.3. Yno1p and the Pheromone Response

The addition of non-physiological concentrations of alpha-factor results in a burst of ROS production [34]. In fact, we found that most of the ROS produced in this setting was dependent on the yeast NADPH oxidase. The wild type strain BY4741 showed a >2-fold increase in DHE fluorescence after addition of 100 µg/mL alpha factor. In the ∆yno1 strain a lower increase in ROS was observed (Figure 5A).
Surprisingly, the formation of shmoos (Figure 5B) was not affected by a deletion of YNO1. Although the ROS level decreased in the ∆yno1 deletion background, the survival rate was not increased, but on the contrary slightly decreased when compared to the wild type (Figure 5C). Confirming the results of Severin and Hyman [34], an overdose of alpha factor initiates a process that kills approximately 50% of cells independent of the genetic background.

3.4. Evaluating the Role of Yno1p in Regulating Filamentous Growth

In addition to regulating the HOG pathway and the response to pheromone, Cdc42p and Ste20p also regulate the filamentous growth pathway [46], which is one of the signaling pathways that induces invasive/filamentous growth in response to nutrient limitation [27]. Filamentous growth in yeast can be induced in several ways. “Fusel” alcohols, such as isoamyl alcohol (IAA), can induce filamentous growth [73,74], as does growth in non-preferred carbon (e.g., galactose) [75] and nitrogen sources [46]. Both 0.5% isoamyl alcohol (IAA) (Figure 6A) and 2% galactose (Figure 6B) induced the Yno1p reporter (of PYNO1 ∆yno1::GFP-HIS3). To confirm this result, YNO1 was genomically tagged with GFP (BY4741 YNO1-GFP::HIS3) and analyzed by immunoblot analysis. Galactose or IAA treatment caused an increase in the level of Yno1p-GFP (2.5-fold and 9 fold, respectively) (Figure 6C). Deletion of STE20 caused a reduction in Yno1p expression in SC-galactose media (Figure 6B). An Yno1p reporter signal was still detectable in the ∆ste20 background after addition of IAA. A downstream target of the yeast MAP kinase cascade consisting of several serine/threonine kinases (Ste20p → Ste11p → Ste7p → Kss1p) are the transcription factors Tec1p and Ste12p [27]. Similar to Msn2p, Ste12p is predicted to bind to the YNO1 promoter region [41,76]. Under the induction conditions tested (galactose and IAA), deletion of STE20 resulted in reduced YNO1 expression (Figure 6A,B). This result indicates that YNO1 expression may be regulated by the MAP kinase cascade that regulates filamentous growth.
Filamentous growth is accompanied by an elongated morphology that results from a delay in the G1 and G2-phases of the cell cycle, a change in cell polarity, and enhanced cell–cell adhesion mediated by the adhesion/flocculin Flo11p. Collectively, these and other changes promote increased invasion of cells into substrates [27,74]. Due to the fact that filamentous growth is strictly dependent on Flo11p [77], and endogenous FLO11 expression is low in BY4741 [78], the filamentous strain background (Σ1278b) was chosen for further experiments [46]. The YNO1 gene was disrupted in an otherwise wild type filamentous strain by homologous recombination of a PCR-amplified KlURA3 cassette containing primer sequences to direct integration at the YNO1 locus. As a first test, the plate-washing assay [79] was performed to test the role of Yno1p in filamentous growth. Yeast cells can penetrate semi-solid agar media and are resistant to removal from agar plates by rinsing with water, by an assay called the plate-washing assay [22]. By this test, the ∆yno1 strain was defective for invasive growth (Figure 7A).
The invasive growth defect of the ∆yno1 strain was less severe than a strain lacking a functional MAP kinase cascade (∆ste11 strain). The effects on cell morphology were also examined. Galactose-induced filamentous growth resulted in the formation of elongated cells in the wild type strain but not the ∆yno1 mutant (Figure 7D). Cells overexpressing YNO1 were also examined. Wild type cells of the Σ1278b background were transformed with a control plasmid, pYES2 or a plasmid that overexpresses YNO1, pYES2-YNO1 (for details see [5]). Compared to the strain harboring the empty vector (pYES2), the YNO1 overexpression construct (pYES2-YNO1) improved invasive growth (Figure 7B). The single cell invasive growth assay, which provides information about budding pattern and cell elongation [49], was also performed. Under conditions that induce filamentous growth (10 µL of 20% galactose plated on a SC-Ura medium), cells overexpressing YNO1 showed hyper-polarized cell morphologies (Figure 7E, arrows).
Based on the phenotypes on wiskostatin discussed above, we wanted to test if Yno1p might impact actin polymerization during filamentous growth and whether hydrogen peroxide plays some role. The LAS17 ORF was cloned into the vector p416GPD, which presumably results in high levels of the N-WASp homologue. This construct was introduced into wild type cells and the ∆yno1 mutant in the ∑1278b strain background. Here, Las17p overexpression restored invasive growth of the ∆yno1 mutant (Figure 7C).
Previously, we have shown that the ∆yno1 mutant has less stable F-actin cables. Addition of hydrogen peroxide promotes actin nucleation and can compensate for the loss of Yno1p [5]. We found that low doses of hydrogen peroxide (0.08 mM H2O2) improved the invasive growth of the ∆yno1 strain (Figure 8). This result supports the idea that reduced superoxide/hydrogen peroxide underlies the invasive growth defect of the ∆yno1 mutant. Higher doses of hydrogen peroxide (0.8 mM H2O2) further improved invasive growth. In a ∆ste11 mutant, addition of hydrogen peroxide had no effect. Therefore, Yno1p-dependent control of ROS is a component of the filamentous growth response in yeast.

3.5. Yno1p, Actin Polymerization and Filamentous Growth

We also tested the effect of Yno1p on the F-actin system during filamentous growth using a collection of rho0 Σ1278b strains expressing Abp140mRFP from chromosomal sites. To obtain exponentially growing cells for investigation, we grew the strains in glucose-containing synthetic media (SC-Ura) to which 20% galactose was added (up to 3%). Galactose induced not only the invasive growth in wild type rho0 strains, but also led to an overproduction of Yno1p in a particular strain harboring an extra copy of YNO1 on the vector pYES2. Since the F-actin system is highly dynamic, we analyzed the rearrangement of Abp140mRFP in corresponding strains at a single cortical layer in time (Figure 9).
We found that wild type cells display a dynamic system of actin patches and filaments. Similar to WT cells, the ∆yno1 cells did not display a loss of cortical actin cables. These cells were usually enlarged, and their enlargement was associated with an increased number of cortical actin cables, and their increased dynamics. The enlarged and round morphology of the ∆yno1 mutant might account for its invasive growth defect. In contrast, the overproduction of Yno1 in this genetic background cells led to a subsequent accumulation of F-actin packages moving along the cortical actin filaments (see Supplementary Movies). Our experiments suggest that the localized activity of Yno1p is required for stabilization of cortical bulk of actin, which would be expected to promote the increase in cell polarization that occurs during filamentous growth.

4. Discussion

A longstanding and popular paradigm has been that ROS are detrimental for the cell. In 1956, Denham Harman proposed his famous “Free Radical of Aging Theory” [80]. In this pioneering publication, it was proposed that irradiation of cells resulted in the emergence of ROS thus leading to cellular damage, DNA mutations, cancer, and finally aging. By the turn of the century, this theory was challenged by several key discoveries. Mitohormesis describes a phenomenon in which impaired glycolysis leads to a stimulation of mitochondrial respiration attributed with an increased ROS production. Surprisingly, this surplus of ROS resulted in the increase of lifespan in the model worm C. elegans [81]. The “aging-ROS” contradiction is the focus of a well-written review [82]. It is now becoming increasingly clear that ROS, although detrimental in some settings, can also act as important signaling molecules.
In fungi, both ROS and NADPH oxidases fulfill important signaling functions that mainly contribute to cellular differentiation. ROS generation is connected to filamentous growth by several different mechanisms [83,84,85], although the mechanisms by which ROS generation is regulated remains incompletely explored. In filamentous fungi, such as Aspergillus nidulans, Podospora anserina, and Neurospora crassa, NADPH oxidases and, thus, ROS are involved in the development of fruiting bodies (cleistothecia) [86,87,88,89]. In the grass endosymbiont Epichloe festucae, NADPH oxidases are needed for asexual spore formation and polarized growth including the formation of hyphae [90]. The NADPH oxidase NoxA functions in a complex with the NOX regulator NoxR. NoxR fulfills the function of a scaffold that establishes contact to BemA and Cdc24 (a GEF for Cdc42) that are essential for polarized growth [90].
These observations are consistent with our findings in S. cerevisiae. It was previously demonstrated that the yeast NADPH oxidase Yno1p is involved in both the stabilization of Yck1/2p and localization of Ras2p [8,15]. In some settings, Ras2p has been shown to regulate the small (monomeric) Rho-like GTPase Cdc42, which is a “master regulator” of polarized growth [31]. Deduced from several synthetic lethality screens and loss-of-function studies a genetic interaction between Bem2p/Cdc42p and Yck1/2p can also be speculated [91,92,93]. In fact, we observed a genetic interaction between Bem2p (one of the GAPs for Cdc42p [94]) and Yno1p. The ∆bem2 ∆yno1 double mutant was hypersensitive to wiskostatin, an F-actin inhibitor. In general, most phenotypes of Yno1p are deeply interwoven with the nucleation of actin. Cdc42p mediates actin polymerization via a complex interplay between the Las17p/Vrp1p dimer, and the Arp2/3 complex [95].
Deletion of YNO1 increased the sensitivity to wiskostatin in a ∆vrp1 or ∆las17 background. This result indicates that Yno1p activity and Las17p/Vrp1 probably act in parallel pathways during regulation of the actin cytoskeleton. However, we could also observe that a double deletion of LAS17 and VRP1 is synthetic lethal.
During osmotic stress, the actin cytoskeleton is also reorganized [40]. We show that addition of 1 M NaCl induced the transcription of the YNO1 gene, which was dependent on the transcription factor Msn2p. Vacuolar fragmentation, which occurs in response to osmotic shock and is dependent on actin polymerization [42,96], was more severe and lasted for a longer time in the ∆yno1 mutant. During osmotic stress, Yno1p interacts with Sod1p to produce hydrogen peroxide. A ∆yno1 ∆sod1 double mutant shows an increased wiskostatin sensitivity compared to the respective single gene deletions. Recently, it was demonstrated that vacuoles in a ∆sod1 background show elevated vacuolar fragmentation [97]. Experiments in the plant Arabidopsis thaliana demonstrate that the importance of ROS as second messengers in response to hyperosmotic shock are evolutionary conserved [98]. Our data also indicate that Yno1p via the activation of Cdc42p regulates its own transcription, actin polymerization and osmotic stress response in parallel. Such a feed forward situation leads to an increase in Yno1p activity and the resulting burst of ROS would lead to cellular damage. Such a system could work well if there is also a mechanism of deactivation after the adaptation phase. This particular positive feedback loop is eventually stopped by the ERAD as previously demonstrated for Yno1p [15]. Eventually also catalases are involved in the removal of a H2O2 excess and thus “over signaling”.
The actin cytoskeleton is also reorganized during morphogenetic responses that are controlled by signal transduction pathways. One example is filamentous/invasive growth, which is controlled by many pathways, including a Cdc42p-dependent MAPK pathway, and which involves reorganization of the actin cytoskeleton (Figure 10) [20]. Fusel alcohols and non-preferred carbon sources that induce invasive growth [27] were also found to induce expression of YNO1. Interestingly, deletion of one of the transcription factors that regulates the fMAPK pathway, STE12, reduced the transcriptional induction of YNO1 to galactose, but not to IAA (Figure 10). Therefore, the expression of YNO1 might be induced by multiple triggers of filamentous/invasive growth.
Yno1p was required for invasive growth, which might result from hydrogen peroxide production, which leads to altered actin filament assembly. Addition of H2O2 improved invasive growth in cells lacking Yno1p. Moreover, overexpression of Las17p complemented the invasive growth defect seen in cells lacking Yno1p (Figure 10). Time-lapse microscopy of Abp140mRFP-labeled cells revealed that overexpression of Yno1p induced formation of F-actin bodies in a similar way as described previously by increased concentration of added H2O2 [99]. These stabile actin aggregates resemble oxidized actin bodies, which may contain various actin-binding proteins. The question remains how the formation of these F-actin structures is related to invasive growth of this particular strain. A role for NADPH oxidases in the regulation of filamentous growth may be conserved among fungi. The NADPH oxidase FRE8 in C. albicans leads to a burst of ROS that is a prerequisite for morphological changes during invasive growth and pathogenicity [6]. Although the closest homologue to Fre8p in C. albicans is not Yno1p but Fre3p in S. cerevisiae, an overexpression of Fre3p in S. cerevisiae did not cause superoxide production, at least under the conditions tested [5]. These findings show that exploring this family of proteins will have value in understanding the role of ROS in fungal dimorphism.
Actin reorganization also occurs during mating projection formation, which is also controlled by a related Cdc42p-dependent MAP kinase pathway. Treatment of Mata cells with alpha factor at levels above physiological concentrations induce apoptotic cell death attributed with a burst of ROS [34]. This could be a trigger to remove cells after unsuccessful mating from the population [100]. In the present work, we show that the ROS is derived from the yeast NADPH oxidase. In the control strain, a~3-fold increase in superoxide levels was detected, whereas in a ∆yno1 strain no such increase was observed. It is assumed that low levels of ROS promote cell survival, whereas high doses of ROS initiate apoptosis [34,101]. Previously, we showed that overexpression of Yno1p resulted in a~9-fold increase in cellular superoxide levels. These high ROS levels correlate with an increased apoptosis and necrosis. Deletion of the yeast metacaspase Yca1p that is an essential component of the yeast apoptotic program diminished the increased cell death that was induced by YNO1 overexpression. This result demonstrates that ROS per se are not detrimental for the cells, but rather fulfill signaling functions depending on location and concentration of ROS. Although the increase of ROS in the ∆yno1 background after addition of alpha factor was not observed, the incidence of apoptosis was still high. In the control strain as well as ∆yno1 strain an unphysiological surplus of alpha factor reduced cell survival to less than 50%. This result indicates that ROS originating from the yeast NADPH oxidase Yno1p fulfills signaling functions independent on the induction of apoptosis and polarized growth/shmooing.

5. Conclusions

Branched actin filaments form at sites of polarized growth and their nucleation is dependent on Las17p/Vrp1p as well as the Arp2/3 complex. In yeast cells, the morphological manifestations of this branched F-actins are so called “actin patches” that have to be clearly distinguished from actin cables. In growing cells, these actin patches mark the site of bud emergence and appear afterwards in the daughter cell [102]. Under specific conditions, actin patches also accumulate at the shmoo tip of mating cells and at the distal tip of pseudohyphal cells and are required for the morphological changes of these cells [20,33]. By comparison, during osmotic stress actin cables are lost and patches redistribute from the daughter to mother cells [103]. A correct localization of these patches at sites of polar growth and in the cortical regions of the cell is mediated by the plasma membrane bound G-protein Cdc42p by a recruitment of Las17p/Vrp1 and Arp2/3 [104]. In the current work, we show that a yeast NADPH oxidase (Yno1p) is involved in this process. Yno1p produces superoxide that is instantly dismutated into hydrogen peroxide by the superoxide dismutase Sod1p [8]. This ROS acts as a signaling molecule and seems to modulate actin functions through Las17p as shown by screens based on the drug wiskostatin, a chemical inhibitor of F-actin polymerization. Accordingly, we could demonstrate that Yno1p overexpression leads to larger large actin patches, whereas in the corresponding deletion background the prevalence for actin cables during filamentous growth is increased. Besides its role in actin nucleation, Cdc42p is a central regulator located upstream of several MAPK pathways that modulate the response during filamentous growth, osmotic stress and mating (Figure 1). In a ∆yno1 deletion strain, reduced ROS levels occur, which presumably underlie the defects in filamentous growth and recovery of vacuolar morphology upon a hyperosmotic stress. The complexity of this regulatory network is further evident by the fact that transcription factors that are controlled by these MAPK pathways stimulate the expression of the YNO1 gene. Further studies in the emerging field of ROS signaling in Saccharomyces cerevisiae may continue to provide information about this type of signaling response.

Supplementary Materials

The following are available online at https://0-www-mdpi-com.brum.beds.ac.uk/2076-3921/10/2/322/s1, Figure S1: GFP-based YNO1-reporter; Supplementary Material (Strains (including construction of the GFP-based YNO1-GFP reporter); media composition; and primer sequences); Supplementary movies (CRY2845; CRY2846; CRY2848).

Author Contributions

M.R., P.J.C. and M.B. concepted the manuscript. M.W., J.H., M.B., C.W.G, P.J.C. and M.R. designed individual experiments. M.W., S.B., B.G., G.P.G., S.S., D.H., J.H. and M.B. performed the experiments. M.W., J.H., M.B., C.W.G, P.J.C. and M.R. analysed data. M.R., P.J.C. and M.W. wrote the manuscript. All authors have read and agreed to the published version of the manuscript.

Funding

The work presented here was supported by grant P33511 of the Austrian Science Fund FWF (to M.R.), P26713 of the FWF to M.B., and NIH grant for P.J.C. (GM098629).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Data is contained within the article or supplementary material.

Acknowledgments

Thanks to M. Whiteway (McGill University) for the plasmids. Thanks to the laboratory members for the suggestions. Open Access Funding by the Austrian Science Fund (FWF).

Conflicts of Interest

The authors declare no conflict of interest.

Abbreviations

ER: endoplasmic reticulum; HOG: hyperosmolarity glycerol response; IAA: isoamyl alcohol; PAK: serine/threonine p21 activated kinase; MAP: mitogen-activated protein; MAPK: mitogen-activated protein kinase; MAPKK: mitogen-activated protein kinase kinase; MAPKKK: mitogen-activated protein kinase kinase kinase; ROS: reactive oxygen species; DIC: Differential interference contrast; NADPH: Nicotinamide adenine dinucleotide; DHE, dihydroethidium; YEPD: yeast extract peptone dextrose; SDS: sodium-dodecyl-sulfate (SDS); PAGE: polyacrylamide gel electrophoresis: PWA: Plate wash assays.

References

  1. Breitenbach, M.; Rinnerthaler, M.; Weber, M.; Breitenbach-Koller, H.; Karl, T.; Cullen, P.; Basu, S.; Haskova, D.; Hasek, J. The defense and signaling role of NADPH oxidases in eukaryotic cells: Review. Wien. Med. Wochenschr. 2018, 168, 286–299. [Google Scholar] [CrossRef] [Green Version]
  2. Royer-Pokora, B.; Kunkel, L.M.; Monaco, A.P.; Goff, S.C.; Newburger, P.E.; Baehner, R.L.; Cole, F.S.; Curnutte, J.T.; Orkin, S.H. Cloning the gene for an inherited human disorder—Chronic granulomatous disease—On the basis of its chromosomal location. Nature 1986, 322, 32–38. [Google Scholar] [CrossRef] [Green Version]
  3. Thomas, D.C. The phagocyte respiratory burst: Historical perspectives and recent advances. Immunol. Lett. 2017, 192, 88–96. [Google Scholar] [CrossRef] [PubMed]
  4. Babior, B.M.; Kipnes, R.S.; Curnutte, J.T. Biological Defense Mechanisms the production by leukocytes of superoxide, a potential bactericidal agent. J. Immunol. 2014, 193, 5359–5362. [Google Scholar] [CrossRef] [PubMed]
  5. Rinnerthaler, M.; Buttner, S.; Laun, P.; Heeren, G.; Felder, T.K.; Klinger, H.; Weinberger, M.; Stolze, K.; Grousl, T.; Hasek, J.; et al. Yno1p/Aim14p, a NADPH-oxidase ortholog, controls extramitochondrial reactive oxygen species generation, apoptosis, and actin cable formation in yeast. Proc. Natl. Acad. Sci. USA 2012, 109, 8658–8663. [Google Scholar] [CrossRef] [Green Version]
  6. Rossi, D.C.P.; Gleason, J.E.; Sanchez, H.; Schatzman, S.S.; Culbertson, E.M.; Johnson, C.J.; McNees, C.A.; Coelho, C.; Nett, J.E.; Andes, D.R.; et al. Candida albicans FRE8 encodes a member of the NADPH oxidase family that produces a burst of ROS during fungal morphogenesis. PLoS Pathog. 2017, 13. [Google Scholar] [CrossRef]
  7. Hajjar, C.; Cherrier, M.V.; Mirandela, G.D.; Petit-Hartlein, I.; Stasia, M.J.; Fontecilla-Camps, J.C.; Fieschi, F.; Dupuy, J. The NOX Family of Proteins Is Also Present in Bacteria. MBio 2017, 8. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  8. Reddi, A.R.; Culotta, V.C. SOD1 integrates signals from oxygen and glucose to repress respiration. Cell 2013, 152, 224–235. [Google Scholar] [CrossRef] [Green Version]
  9. Roth, A.F.; Papanayotou, I.; Davis, N.G. The yeast kinase Yck2 has a tripartite palmitoylation signal. Mol. Biol. Cell 2011, 22, 2702–2715. [Google Scholar] [CrossRef]
  10. Babu, P.; Bryan, J.D.; Panek, H.R.; Jordan, S.L.; Forbrich, B.M.; Kelley, S.C.; Colvin, R.T.; Robinson, L.C. Plasma membrane localization of the Yck2p yeast casein kinase 1 isoform requires the C-terminal extension and secretory pathway function. J. Cell Sci. 2002, 115, 4957–4968. [Google Scholar] [CrossRef] [Green Version]
  11. Snowdon, C.; Johnston, M. A novel role for yeast casein kinases in glucose sensing and signaling. Mol. Biol. Cell 2016, 27, 3369–3375. [Google Scholar] [CrossRef]
  12. Alvaro, C.G.; O’Donnell, A.F.; Prosser, D.C.; Augustine, A.A.; Goldman, A.; Brodsky, J.L.; Cyert, M.S.; Wendland, B.; Thorner, J. Specific alpha-arrestins negatively regulate Saccharomyces cerevisiae pheromone response by down-modulating the G-protein-coupled receptor Ste2. Mol. Cell Biol. 2014, 34, 2660–2681. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  13. Bartels, D.J.; Mitchell, D.A.; Dong, X.W.; Deschenes, R.J. Erf2, a novel gene product that affects the localization and palmitoylation of Ras2 in Saccharomyces cerevisiae. Mol. Cell Biol. 1999, 19, 6775–6787. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  14. Kim, J.H.; Johnston, M. Two glucose-sensing pathways converge on Rgt1 to regulate expression of glucose transporter genes in Saccharomyces cerevisiae. J. Biol Chem. 2006, 281, 26144–26149. [Google Scholar] [CrossRef] [Green Version]
  15. Leadsham, J.E.; Sanders, G.; Giannaki, S.; Bastow, E.L.; Hutton, R.; Naeimi, W.R.; Breitenbach, M.; Gourlay, C.W. Loss of Cytochrome c Oxidase Promotes RAS-Dependent ROS Production from the ER Resident NADPH Oxidase, Yno1p, in Yeast. Cell Metab. 2013, 18, 279–286. [Google Scholar] [CrossRef] [Green Version]
  16. Gale, C.A.; Bendel, C.M.; McClellan, M.; Hauser, M.; Becker, J.M.; Berman, J.; Hostetter, M.K. Linkage of adhesion, filamentous growth, and virulence in Candida albicans to a single gene, INT1. Science 1998, 279, 1355–1358. [Google Scholar] [CrossRef]
  17. Whiteway, M.; Bachewich, C. Morphogenesis in Candida albicans. Annu. Rev. Microbiol. 2007, 61, 529–553. [Google Scholar] [CrossRef] [Green Version]
  18. Gimeno, C.J.; Ljungdahl, P.O.; Styles, C.A.; Fink, G.R. Unipolar Cell Divisions in the Yeast Saccharomyces—Cerevisiae Lead to Filamentous Growth—Regulation by Starvation and Ras. Cell 1992, 68, 1077–1090. [Google Scholar] [CrossRef]
  19. Kron, S.J.; Styles, C.A.; Fink, G.R. Symmetric cell division in pseudohyphae of the yeast Saccharomyces cerevisiae. Mol. Biol. Cell 1994, 5, 1003–1022. [Google Scholar] [CrossRef]
  20. Cali, B.M.; Doyle, T.C.; Botstein, D.; Fink, G.R. Multiple functions for actin during filamentous growth of Saccharomyces cerevisiae. Mol. Biol. Cell 1998, 9, 1873–1889. [Google Scholar] [CrossRef] [PubMed]
  21. Taheri, N.; Kohler, T.; Braus, G.H.; Mosch, H.U. Asymmetrically localized Bud8p and Bud9p proteins control yeast cell polarity and development. EMBO J. 2000, 19, 6686–6696. [Google Scholar] [CrossRef] [PubMed]
  22. Roberts, R.L.; Fink, G.R. Elements of a single MAP kinase cascade in Saccharomyces cerevisiae mediate two developmental programs in the same cell type: Mating and invasive growth. Genes Dev. 1994, 8, 2974–2985. [Google Scholar] [CrossRef] [Green Version]
  23. Robertson, L.S.; Fink, G.R. The three yeast A kinases have specific signaling functions in pseudohyphal growth. Proc. Natl. Acad. Sci. USA 1998, 95, 13783–13787. [Google Scholar] [CrossRef] [Green Version]
  24. Lorenz, M.C.; Heitman, J. Yeast pseudohyphal growth is regulated by GPA2, a G protein alpha homolog. EMBO J. 1997, 16, 7008–7018. [Google Scholar] [CrossRef] [Green Version]
  25. Pan, X.; Heitman, J. Cyclic AMP-dependent protein kinase regulates pseudohyphal differentiation in Saccharomyces cerevisiae. Mol. Cell Biol. 1999, 19, 4874–4887. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  26. Lamb, T.M.; Mitchell, A.P. The transcription factor Rim101p governs ion tolerance and cell differentiation by direct repression of the regulatory genes NRG1 and SMP1 in Saccharomyces cerevisiae. Mol. Cell Biol. 2003, 23, 677–686. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  27. Cullen, P.J.; Sprague, G.F. The Regulation of Filamentous Growth in Yeast. Genetics 2012, 190, 23–49. [Google Scholar] [CrossRef]
  28. Cullen, P.J.; Sabbagh, W.; Graham, E.; Irick, M.M.; van Olden, E.K.; Neal, C.; Delrow, J.; Bardwell, L.; Sprague, G.F. A signaling mucin at the head of the Cdc42- and MAPK-dependent filamentous growth pathway in yeast. Gene Dev. 2004, 18, 1695–1708. [Google Scholar] [CrossRef] [Green Version]
  29. Peter, M.; Neiman, A.M.; Park, H.O.; vanLohuizen, M.; Herskowitz, I. Functional analysis of the interaction between the small GTP binding protein Cdc42 and the Ste20 protein kinase in yeast. EMBO J. 1996, 15, 7046–7059. [Google Scholar] [CrossRef]
  30. Gancedo, J.M. Control of pseudohyphae formation in Saccharomyces cerevisiae. FEMS Microbiol. Rev. 2001, 25, 107–123. [Google Scholar] [CrossRef]
  31. Mosch, H.U.; Roberts, R.L.; Fink, G.R. Ras2 signals via the Cdc42/Ste20/mitogen-activated protein kinase module to induce filamentous growth in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 1996, 93, 5352–5356. [Google Scholar] [CrossRef] [Green Version]
  32. Chen, R.E.; Thorner, J. Function and regulation in MAPK signaling pathways: Lessons learned from the yeast Saccharomyces cerevisiae. Biochim. Biophys. Acta 2007, 1773, 1311–1340. [Google Scholar] [CrossRef] [Green Version]
  33. Smith, M.G.; Swamy, S.R.; Pon, L.A. The life cycle of actin patches in mating yeast. J. Cell Sci. 2001, 114, 1505–1513. [Google Scholar] [PubMed]
  34. Severin, F.F.; Hyman, A.A. Pheromone induces programmed cell death in S. cerevisiae. Curr. Biol. 2002, 12, R233–235. [Google Scholar] [CrossRef] [Green Version]
  35. Saito, H. Regulation of cross-talk in yeast MAPK signaling pathways. Curr. Opin. Microbiol. 2010, 13, 677–683. [Google Scholar] [CrossRef]
  36. Nishimura, A.; Yamamoto, K.; Oyama, M.; Kozuka-Hata, H.; Saito, H.; Tatebayashi, K. Scaffold Protein Ahk1, Which Associates with Hkr1, Sho1, Ste11, and Pbs2, Inhibits Cross Talk Signaling from the Hkr1 Osmosensor to the Kss1 Mitogen-Activated Protein Kinase. Mol. Cell Biol. 2016, 36, 1109–1123. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  37. Pitoniak, A.; Birkaya, B.; Dionne, H.M.; Vadaie, N.; Cullen, P.J. The Signaling Mucins Msb2 and Hkr1 Differentially Regulate the Filamentation Mitogen-activated Protein Kinase Pathway and Contribute to a Multimodal Response. Mol. Biol. Cell 2009, 20, 3101–3114. [Google Scholar] [CrossRef] [Green Version]
  38. Tanaka, K.; Tatebayashi, K.; Nishimura, A.; Yamamoto, K.; Yang, H.Y.; Saito, H. Yeast Osmosensors Hkr1 and Msb2 Activate the Hog1 MAPK Cascade by Different Mechanisms. Sci. Signal. 2014, 7. [Google Scholar] [CrossRef] [PubMed]
  39. Tatebayashi, K.; Tanaka, K.; Yang, H.Y.; Yamamoto, K.; Matsushita, Y.; Tomida, T.; Imai, M.; Saito, H. Transmembrane mucins Hkr1 and Msb2 are putative osmosensors in the SHO1 branch of yeast HOG pathway. EMBO J. 2007, 26, 3521–3533. [Google Scholar] [CrossRef] [Green Version]
  40. Hohmann, S. Osmotic stress signaling and osmoadaptation in Yeasts. Microbiol. Mol. Biol. R. 2002, 66, 300–372. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  41. Monteiro, P.T.; Oliveira, J.; Pais, P.; Antunes, M.; Palma, M.; Cavalheiro, M.; Galocha, M.; Godinho, C.P.; Martins, L.C.; Bourbon, N.; et al. YEASTRACT plus: A portal for cross-species comparative genomics of transcription regulation in yeasts. Nucleic Acids Res. 2020, 48, D642–D649. [Google Scholar] [CrossRef] [PubMed]
  42. Isgandarova, S.; Jones, L.; Forsberg, D.; Loncar, A.; Dawson, J.; Tedrick, K.; Eitzen, G. Stimulation of actin polymerization by vacuoles via Cdc42p-dependent signaling. J. Biol. Chem. 2007, 282, 30466–30475. [Google Scholar] [CrossRef] [Green Version]
  43. Li, S.C.; Kane, P.M. The yeast lysosome-like vacuole: Endpoint and crossroads. BBA-Mol. Cell Res. 2009, 1793, 650–663. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  44. Brachmann, C.B.; Davies, A.; Cost, G.J.; Caputo, E.; Li, J.C.; Hieter, P.; Boeke, J.D. Designer deletion strains derived from Saccharomyces cerevisiae S288C: A useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 1998, 14, 115–132. [Google Scholar] [CrossRef]
  45. Van Dyk, D.; Pretorius, I.S.; Bauer, F.F. Mss11p is a central element of the regulatory network that controls FLO11 expression and invasive growth in Saccharomyces cerevisiae. Genetics 2005, 169, 91–106. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  46. Klinger, H.; Rinnerthaler, M.; Lam, Y.T.; Laun, P.; Heeren, G.; Klocker, A.; Simon-Nobbe, B.; Dickinson, J.R.; Dawes, I.W.; Breitenbach, M. Quantitation of (a)symmetric inheritance of functional and of oxidatively damaged mitochondrial aconitase in the cell division of old yeast mother cells. Exp. Gerontol. 2010, 45, 533–542. [Google Scholar] [CrossRef]
  47. Streubel, M.K.; Bischof, J.; Weiss, R.; Duschl, J.; Liedl, W.; Wimmer, H.; Breitenbach, M.; Weber, M.; Geltinger, F.; Richter, K.; et al. Behead and live long or the tale of cathepsin L. Yeast 2018, 35, 237–249. [Google Scholar] [CrossRef] [Green Version]
  48. Cullen, P.J.; Sprague, G.F. Glucose depletion causes haploid invasive growth in yeast. Proc. Natl. Acad. Sci. USA 2000, 97, 13619–13624. [Google Scholar] [CrossRef] [Green Version]
  49. Zupan, J.; Raspor, P. Quantitative agar-invasion assay. J. Microbiol. Methods 2008, 73, 100–104. [Google Scholar] [CrossRef]
  50. Basu, S.; Vadaie, N.; Prabhakar, A.; Li, B.; Adhikari, H.; Pitoniak, A.; Chow, J.; Chavel, C.A.; Cullen, P.J. Spatial landmarks regulate a Cdc42-dependent MAPK pathway to control differentiation and the response to positional compromise. Proc. Natl. Acad. Sci. USA 2016, 113, E2019–2028. [Google Scholar] [CrossRef] [Green Version]
  51. Michaillat, L.; Mayer, A. Identification of Genes Affecting Vacuole Membrane Fragmentation in Saccharomyces cerevisiae. PLoS ONE 2013, 8, e54160. [Google Scholar] [CrossRef] [Green Version]
  52. Heeren, G.; Rinnerthaler, M.; Laun, P.; von Seyerl, P.; Kossler, S.; Klinger, H.; Hager, M.; Bogengruber, E.; Jarolim, S.; Simon-Nobbe, B.; et al. The mitochondrial ribosomal protein of the large subunit, Afo1p, determines cellular longevity through mitochondrial back-signaling via TOR1. Aging-Us 2009, 1, 622–636. [Google Scholar] [CrossRef] [Green Version]
  53. Grant, C.M.; MacIver, F.H.; Dawes, I.W. Mitochondrial function is required for resistance to oxidative stress in the yeast Saccharomyces cerevisiae. FEBS Lett. 1997, 410, 219–222. [Google Scholar] [CrossRef]
  54. Pyatrikas, D.V.; Fedoseeva, I.V.; Varakina, N.N.; Rusaleva, T.M.; Stepanov, A.V.; Fedyaeva, A.V.; Borovskii, G.B.; Rikhvanov, E.G. Relation between cell death progression, reactive oxygen species production and mitochondrial membrane potential in fermenting Saccharomyces cerevisiae cells under heat-shockconditions. FEMS Microbiol. Lett. 2015, 362. [Google Scholar] [CrossRef] [Green Version]
  55. Fernandes, D.C.; Wosniak, J., Jr.; Pescatore, L.A.; Bertoline, M.A.; Liberman, M.; Laurindo, F.R.; Santos, C.X. Analysis of DHE-derived oxidation products by HPLC in the assessment of superoxide production and NADPH oxidase activity in vascular systems. Am. J. Physiol. Cell Physiol. 2007, 292, C413–422. [Google Scholar] [CrossRef] [PubMed]
  56. Dikalov, S.; Nazarewicz, R.; Panov, A.; Harrison, D.G.; Dikalova, A. Crosstalk Between Mitochondrial ROS and NADPH Oxidases in Cardiovascular and Degenerative Diseases: Application of Mitochondria-Targeted Antioxidants. Free Radic. Bio. Med. 2011, 51, S85–S86. [Google Scholar] [CrossRef]
  57. Zorov, D.B.; Juhaszova, M.; Sollott, S.J. Mitochondrial Reactive Oxygen Species (Ros) and Ros-Induced Ros Release. Physiol. Rev. 2014, 94, 909–950. [Google Scholar] [CrossRef] [Green Version]
  58. Yi, D.G.; Hong, S.; Huh, W.K. Mitochondrial dysfunction reduces yeast replicative lifespan by elevating RAS-dependent ROS production by the ER-localized NADPH oxidase Yno1. PLoS ONE 2018, 13, e0198619. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  59. Mateus, C.; Avery, S.V. Destabilized green fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry. Yeast 2000, 16, 1313–1323. [Google Scholar] [CrossRef]
  60. Peterson, J.R.; Bickford, L.C.; Morgan, D.; Kim, A.S.; Ouerfelli, O.; Kirschner, M.W.; Rosen, M.K. Chemical inhibition of N-WASP by stabilization of a native autoinhibited conformation. Nat. Struct. Mol. Biol. 2004, 11, 747–755. [Google Scholar] [CrossRef]
  61. Madania, A.; Dumoulin, P.; Grava, S.; Kitamoto, H.; Scharer-Brodbeck, C.; Soulard, A.; Moreau, V.; Winsor, B. The Saccharomyces cerevisiae homologue of human Wiskott-Aldrich syndrome protein Las17p interacts with the Arp2/3 complex. Mol. Biol. Cell 1999, 10, 3521–3538. [Google Scholar] [CrossRef] [Green Version]
  62. Aspenstrom, P. The verprolin family of proteins: Regulators of cell morphogenesis and endocytosis. FEBS Lett. 2005, 579, 5253–5259. [Google Scholar] [CrossRef] [Green Version]
  63. Tyler, J.J.; Allwood, E.G.; Ayscough, K.R. WASP family proteins, more than Arp2/3 activators. Biochem. Soc. Trans. 2016, 44, 1339–1345. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  64. Raitt, D.C.; Posas, F.; Saito, H. Yeast Cdc42 GTPase and Ste20 PAK-like kinase regulate Sho1-dependent activation of the Hog1 MAPK pathway. EMBO J. 2000, 19, 4623–4631. [Google Scholar] [CrossRef]
  65. Leberer, E.; Dignard, D.; Harcus, D.; Thomas, D.Y.; Whiteway, M. The Protein-Kinase Homolog Ste20p Is Required to Link the Yeast Pheromone Response G-Protein Beta-Gamma Subunits to Downstream Signaling Components. EMBO J. 1992, 11, 4815–4824. [Google Scholar] [CrossRef]
  66. Huh, G.H.; Damsz, B.; Matsumoto, T.K.; Reddy, M.P.; Rus, A.M.; Ibeas, J.I.; Narasimhan, M.L.; Bressan, R.A.; Hasegawa, P.M. Salt causes ion disequilibrium-induced programmed cell death in yeast and plants. Plant J. 2002, 29, 649–659. [Google Scholar] [CrossRef]
  67. Wadskog, I.; Maldener, C.; Proksch, A.; Madeo, F.; Adler, L. Yeast lacking the SRO7/SOP1-encoded tumor suppressor homologue show increased susceptibility to apoptosis-like cell death on exposure to NaCl stress. Mol. Biol. Cell 2004, 15, 1436–1444. [Google Scholar] [CrossRef] [Green Version]
  68. Rep, M.; Reiser, V.; Gartner, U.; Thevelein, J.M.; Hohmann, S.; Ammerer, G.; Ruis, H. Osmotic stress-induced gene expression in Saccharomyces cerevisiae requires Msn1p and the novel nuclear factor Hot1p. Mol. Cell Biol. 1999, 19, 5474–5485. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  69. Gorner, W.; Durchschlag, E.; Martinez-Pastor, M.T.; Estruch, F.; Ammerer, G.; Hamilton, B.; Ruis, H.; Schuller, C. Nuclear localization of the C2H2 zinc finger protein Msn2p is regulated by stress and protein kinase A activity. Gene Dev. 1998, 12, 586–597. [Google Scholar] [CrossRef]
  70. Berry, D.B.; Gasch, A.P. Stress-activated Genomic Expression Changes Serve a Preparative Role for Impending Stress in Yeast. Mol. Biol. Cell 2008, 19, 4580–4587. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  71. Miermont, A.; Waharte, F.; Hu, S.Q.; McClean, M.N.; Bottani, S.; Leon, S.; Hersen, P. Severe osmotic compression triggers a slowdown of intracellular signaling, which can be explained by molecular crowding. Proc. Natl. Acad. Sci. USA 2013, 110, 5725–5730. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  72. Vida, T.A.; Emr, S.D. A New Vital Stain for Visualizing Vacuolar Membrane Dynamics and Endocytosis in Yeast. J. Cell Biol. 1995, 128, 779–792. [Google Scholar] [CrossRef]
  73. Dickinson, J.R. ‘Fusel’ alcohols induce hyphal-like extensions and pseudohyphal formation in yeasts. Microbiology 1996, 142, 1391–1397. [Google Scholar] [CrossRef] [Green Version]
  74. Lorenz, M.C.; Cutler, N.S.; Heitman, J. Characterization of alcohol-induced filamentous growth in Saccharomyces cerevisiae. Mol. Biol. Cell 2000, 11, 183–199. [Google Scholar] [CrossRef] [Green Version]
  75. Adhikari, H.; Cullen, P.J. Metabolic Respiration Induces AMPK- and Ire1p-Dependent Activation of the p38-Type HOG MAPK Pathway. PLoS Genet. 2014, 10. [Google Scholar] [CrossRef] [PubMed]
  76. Madhani, H.D.; Galitski, T.; Lander, E.S.; Fink, G.R. Effectors of a developmental mitogen-activated protein kinase cascade revealed by expression signatures of signaling mutants. Proc. Natl. Acad. Sci. USA 1999, 96, 12530–12535. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  77. Palecek, S.P.; Parikh, A.S.; Kron, S.J. Genetic analysis reveals that FLO11 upregulation and cell polarization independently regulate invasive growth in Saccharomyces cerevisiae. Genetics 2000, 156, 1005–1023. [Google Scholar] [PubMed]
  78. Purevdorj-Gage, B.; Orr, M.E.; Stoodley, P.; Sheehan, K.B.; Hyman, L.E. The role of FLO11 in Saccharomyces cerevisiae biofilm development in a laboratory based flow-cell system. FEMS Yeast Res. 2007, 7, 372–379. [Google Scholar] [CrossRef] [Green Version]
  79. Cullen, P.J. The plate-washing assay: A simple test for filamentous growth in budding yeast. Cold Spring Harb. Protoc. 2015, 2015, 168–171. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  80. Harman, D. Aging: A theory based on free radical and radiation chemistry. J. Gerontol. 1956, 11, 298–300. [Google Scholar] [CrossRef] [Green Version]
  81. Schulz, T.J.; Zarse, K.; Voigt, A.; Urban, N.; Birringer, M.; Ristow, M. Glucose restriction extends Caenorhabditis elegans life span by inducing mitochondrial respiration and increasing oxidative stress. Cell Metab. 2007, 6, 280–293. [Google Scholar] [CrossRef] [Green Version]
  82. Lapointe, J.; Hekimi, S. When a theory of aging ages badly. Cell Mol. Life Sci. 2010, 67, 1–8. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  83. Xu, N.; Cheng, X.X.; Yu, Q.L.; Qian, K.F.; Ding, X.H.; Liu, R.M.; Zhang, B.; Xing, L.J.; Li, M.C. Aft2, a Novel Transcription Regulator, Is Required for Iron Metabolism, Oxidative Stress, Surface Adhesion and Hyphal Development in Candida albicans. PLoS ONE 2013, 8. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  84. Zhang, Y.; Wang, L.; Liang, S.; Zhang, P.; Kang, R.; Zhang, M.; Wang, M.; Chen, L.; Yuan, H.; Ding, S.; et al. FpDep1, a component of Rpd3L histone deacetylase complex, is important for vegetative development, ROS accumulation, and pathogenesis in Fusarium pseudograminearum. Fungal Genet. Biol. 2020, 135, 103299. [Google Scholar] [CrossRef] [PubMed]
  85. Basso, V.; Znaidi, S.; Lagage, V.; Cabral, V.; Schoenherr, F.; LeibundGut-Landmann, S.; d’Enfert, C.; Bachellier-Bassi, S. The two-component response regulator Skn7 belongs to a network of transcription factors regulating morphogenesis in Candida albicans and independently limits morphogenesis-induced ROS accumulation. Mol. Microbiol. 2017, 106, 157–182. [Google Scholar] [CrossRef] [Green Version]
  86. Breitenbach, M.; Weber, M.; Rinnerthaler, M.; Karl, T.; Breitenbach-Koller, L. Oxidative Stress in Fungi: Its Function in Signal Transduction, Interaction with Plant Hosts, and Lignocellulose Degradation. Biomolecules 2015, 5, 318–342. [Google Scholar] [CrossRef] [Green Version]
  87. Malagnac, F.; Lalucque, H.; Lepere, G.; Silar, P. Two NADPH oxidase isoforms are required for sexual reproduction and ascospore germination in the filamentous fungus Podospora anserina. Fungal Genet. Biol. 2004, 41, 982–997. [Google Scholar] [CrossRef]
  88. Lara-Ortiz, T.; Riveros-Rosas, H.; Aguirre, J. Reactive oxygen species generated by microbial NADPH oxidase NoxA regulate sexual development in Aspergillus nidulans. Mol. Microbiol. 2003, 50, 1241–1255. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  89. Cano-Dominguez, N.; Alvarez-Delfin, K.; Hansberg, W.; Aguirre, J. NADPH oxidases NOX-1 and NOX-2 require the regulatory subunit NOR-1 to control cell differentiation and growth in Neurospora crassa. Eukaryot. Cell 2008, 7, 1352–1361. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  90. Kayano, Y.; Tanaka, A.; Akano, F.; Scott, B.; Takemoto, D. Differential roles of NADPH oxidases and associated regulators in polarized growth, conidiation and hyphal fusion in the symbiotic fungus Epichloe festucae. Fungal Genet. Biol. 2013, 56, 87–97. [Google Scholar] [CrossRef] [PubMed]
  91. Costanzo, M.; Baryshnikova, A.; Bellay, J.; Kim, Y.; Spear, E.D.; Sevier, C.S.; Ding, H.; Koh, J.L.; Toufighi, K.; Mostafavi, S.; et al. The genetic landscape of a cell. Science 2010, 327, 425–431. [Google Scholar] [CrossRef] [Green Version]
  92. Costanzo, M.; VanderSluis, B.; Koch, E.N.; Baryshnikova, A.; Pons, C.; Tan, G.; Wang, W.; Usaj, M.; Hanchard, J.; Lee, S.D.; et al. A global genetic interaction network maps a wiring diagram of cellular function. Science 2016, 353. [Google Scholar] [CrossRef]
  93. Sharifpoor, S.; van Dyk, D.; Costanzo, M.; Baryshnikova, A.; Friesen, H.; Douglas, A.C.; Youn, J.Y.; VanderSluis, B.; Myers, C.L.; Papp, B.; et al. Functional wiring of the yeast kinome revealed by global analysis of genetic network motifs. Genome Res. 2012, 22, 791–801. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  94. Perez, P.; Rincon, S.A. Rho GTPases: Regulation of cell polarity and growth in yeasts. Biochem. J. 2010, 426, 243–253. [Google Scholar] [CrossRef] [Green Version]
  95. Evangelista, M.; Klebl, B.M.; Tong, A.H.Y.; Webb, B.A.; Leeuw, T.; Leberer, E.; Whiteway, M.; Thomas, D.Y.; Boone, C. A role for myosin-I in actin assembly through interactions with Vrp1p, Bee1p, and the Arp2/3 complex. J. Cell Biol. 2000, 148, 353–362. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  96. Tedrick, K.; Trischuk, T.; Lehner, R.; Eitzen, G. Enhanced membrane fusion in sterol-enriched vacuoles bypasses the Vrp1p requirement. Mol. Biol. Cell 2004, 15, 4609–4621. [Google Scholar] [CrossRef] [Green Version]
  97. Montllor-Albalate, C.; Colin, A.E.; Chandrasekharan, B.; Bolaji, N.; Andersen, J.L.; Outten, F.W.; Reddi, A.R. Extra-mitochondrial Cu/Zn superoxide dismutase (Sod1) is dispensable for protection against oxidative stress but mediates peroxide signaling in Saccharomyces cerevisiae. Redox Biol. 2019, 21. [Google Scholar] [CrossRef]
  98. Martiniere, A.; Fiche, J.B.; Smokvarska, M.; Mari, S.; Alcon, C.; Dumont, X.; Hematy, K.; Jaillais, Y.; Nollmann, M.; Maurel, C. Osmotic Stress Activates Two Reactive Oxygen Species Pathways with Distinct Effects on Protein Nanodomains and Diffusion. Plant Physiol. 2019, 179, 1581–1593. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  99. Farah, M.E.; Sirotkin, V.; Haarer, B.; Kakhniashvili, D.; Amberg, D.C. Diverse protective roles of the actin cytoskeleton during oxidative stress. Cytoskeleton 2011, 68, 340–354. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  100. Carmona-Gutierrez, D.; Eisenberg, T.; Buttner, S.; Meisinger, C.; Kroemer, G.; Madeo, F. Apoptosis in yeast: Triggers, pathways, subroutines. Cell Death Differ. 2010, 17, 763–773. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  101. Perrone, G.G.; Tan, S.X.; Dawes, I.W. Reactive oxygen species and yeast apoptosis. Biochim. Biophys. Acta 2008, 1783, 1354–1368. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  102. Karpova, T.S.; McNally, J.G.; Moltz, S.L.; Cooper, J.A. Assembly and function of the actin cytoskeleton of yeast: Relationships between cables and patches. J. Cell Biol. 1998, 142, 1501–1517. [Google Scholar] [CrossRef] [PubMed]
  103. Chowdhury, S.; Smith, K.W.; Gustin, M.C. Osmotic stress and the yeast cytoskeleton: Phenotype-specific suppression of an actin mutation. J. Cell Biol. 1992, 118, 561–571. [Google Scholar] [CrossRef] [PubMed]
  104. Lechler, T.; Jonsdottir, G.A.; Klee, S.K.; Pellman, D.; Li, R. A two-tiered mechanism by which Cdc42 controls the localization and activation of an Arp2/3-activating motor complex in yeast. J. Cell Biol. 2001, 155, 261–270. [Google Scholar] [CrossRef] [PubMed] [Green Version]
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Figure 1. Cdc42-dependent MAPK pathways in yeast. Three yeast MAPK pathways share components, yet induce different responses to different stimuli. Pathway components common to multiple pathways are shown in black. Pathway-specific proteins are colored (red letters: hyperosmolarity glycerol response (HOG) pathway; blue letters: pheromone response; green letters: filamentous growth). Not all components are shown.
Figure 1. Cdc42-dependent MAPK pathways in yeast. Three yeast MAPK pathways share components, yet induce different responses to different stimuli. Pathway components common to multiple pathways are shown in black. Pathway-specific proteins are colored (red letters: hyperosmolarity glycerol response (HOG) pathway; blue letters: pheromone response; green letters: filamentous growth). Not all components are shown.
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Figure 2. A GFP-based YNO1-reporter. (A) Superoxide levels were measured by using the fluorescent probe DHE. The wild type strain BY4741 (rho+) and the petite strain BY4741 (rho0) were analyzed (N = 4–7). In (B–E), the expression of YNO1 was studied by measuring GFP fluorescence. A GFP-HIS3 cassette was used to replace the native YNO1 ORF. In (B), the strains BY4741 ∆yno1::GFP-HIS3 rho+ and BY4741 ∆yno1::GFP-HIS3 rho0 were compared (N = 6). In (C), the expression of YNO1 was analyzed at the transition from late exponential phase to early stationary and stationary phase in the strain BY4741 ∆yno1::GFP-HIS3 (N = 4). (D) shows the expression of YNO1 in the strains BY4741 ∆yno1::GFP-HIS3 rho+ and BY4741 ∆yno1::GFP-HIS3 rho0 after addition of 200 µM wiskostatin (wisko, N = 4). In (E), wiskostatin (200 µM)-induced expression of YNO1 is reversed by addition of 0.5 mM or 1 mM H2O2 (N = 4) (*: p < 0.1; **: p < 0.05; ***: p < 0.01).
Figure 2. A GFP-based YNO1-reporter. (A) Superoxide levels were measured by using the fluorescent probe DHE. The wild type strain BY4741 (rho+) and the petite strain BY4741 (rho0) were analyzed (N = 4–7). In (B–E), the expression of YNO1 was studied by measuring GFP fluorescence. A GFP-HIS3 cassette was used to replace the native YNO1 ORF. In (B), the strains BY4741 ∆yno1::GFP-HIS3 rho+ and BY4741 ∆yno1::GFP-HIS3 rho0 were compared (N = 6). In (C), the expression of YNO1 was analyzed at the transition from late exponential phase to early stationary and stationary phase in the strain BY4741 ∆yno1::GFP-HIS3 (N = 4). (D) shows the expression of YNO1 in the strains BY4741 ∆yno1::GFP-HIS3 rho+ and BY4741 ∆yno1::GFP-HIS3 rho0 after addition of 200 µM wiskostatin (wisko, N = 4). In (E), wiskostatin (200 µM)-induced expression of YNO1 is reversed by addition of 0.5 mM or 1 mM H2O2 (N = 4) (*: p < 0.1; **: p < 0.05; ***: p < 0.01).
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Figure 3. The sensitivity of mutants to wiskostatin by spot test analysis. Serial dilutions (OD600 of 3, 1, 0.3, and 0.1) of the strains BY4741, BY4741 ∆yno1, BY4741 ∆vrp1, BY4741 ∆sod1, BY4741 ∆bem2, BY4741 ∆las17, BY4741 ∆yno1 ∆vrp1, BY4741 ∆yno1 ∆sod1, BY4741 ∆yno1 ∆bem2, and BY4741 ∆yno1 ∆las17 were spotted on SC-glucose plates, SC-glucose +20 µM wiskostatin plates and SC-glucose +15 µM wiskostatin plates. At least three biological replicates were tested on plates containing concentrations of wiskostatin in a range from 0–100 µM. Representative images and concentrations were chosen.
Figure 3. The sensitivity of mutants to wiskostatin by spot test analysis. Serial dilutions (OD600 of 3, 1, 0.3, and 0.1) of the strains BY4741, BY4741 ∆yno1, BY4741 ∆vrp1, BY4741 ∆sod1, BY4741 ∆bem2, BY4741 ∆las17, BY4741 ∆yno1 ∆vrp1, BY4741 ∆yno1 ∆sod1, BY4741 ∆yno1 ∆bem2, and BY4741 ∆yno1 ∆las17 were spotted on SC-glucose plates, SC-glucose +20 µM wiskostatin plates and SC-glucose +15 µM wiskostatin plates. At least three biological replicates were tested on plates containing concentrations of wiskostatin in a range from 0–100 µM. Representative images and concentrations were chosen.
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Figure 4. YNO1 reporter levels and vacuolar morphology in response to osmotic stress. (A) Superoxide levels were measured using the dye DHE in the strains BY4741 rho+, BY4741 rho0, and BY4741 ∆yno1 rho0 (N = 4–7). In (B) the YNO1 expression in the strains BY4741 rho+, BY4741 rho0, BY4741 ∆ste20 rho0 and BY4741 ∆msn2 rho0 was measured using a GFP-reporter (N = 4–6). In (A) and (B), osmotic stress response was induced by the addition of 1 M NaCl (***: p < 0.01). In (C), vacuolar membranes were stained with the lipophilic dye FM4-64. Osmotic stress was induced by addition of 0.4 M NaCl. Scale bars represent 5 µm. The assay was performed in triplicate, and representative images are shown.
Figure 4. YNO1 reporter levels and vacuolar morphology in response to osmotic stress. (A) Superoxide levels were measured using the dye DHE in the strains BY4741 rho+, BY4741 rho0, and BY4741 ∆yno1 rho0 (N = 4–7). In (B) the YNO1 expression in the strains BY4741 rho+, BY4741 rho0, BY4741 ∆ste20 rho0 and BY4741 ∆msn2 rho0 was measured using a GFP-reporter (N = 4–6). In (A) and (B), osmotic stress response was induced by the addition of 1 M NaCl (***: p < 0.01). In (C), vacuolar membranes were stained with the lipophilic dye FM4-64. Osmotic stress was induced by addition of 0.4 M NaCl. Scale bars represent 5 µm. The assay was performed in triplicate, and representative images are shown.
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Figure 5. The role of Yno1p in pheromone response. (A) DHE fluorescence was analyzed in the strains BY4741 and BY4741 ∆yno1 in untreated controls as well as after addition of alpha factor (100 µg/mL) (N = 8). In (B), differential interference contrast microscopy images of shmoos in the BY4741 and BY4741 ∆yno1 background are shown after addition of alpha factor (100 µg/mL). In (C), the survival rate in the strains BY4741 and BY4741 ∆yno1 was calculated by survival plating. Cell death was induced by addition of alpha factor (100 µg/mL). In untreated cells (BY4741 and BY4741 ∆yno1), no difference in survival was observed. For (A,C): each bar represents the comparison between untreated and treated yeast strains (either BY4741 or BY4741 ∆yno1), asterisks above the bars indicate statistical significance between with/without treatment, asterisks above the brackets represent statistical significance between the different yeast strains (BY4741 and BY4741 ∆yno1) upon treatment with alpha-factor (*: p < 0.1; ***: p < 0.01).
Figure 5. The role of Yno1p in pheromone response. (A) DHE fluorescence was analyzed in the strains BY4741 and BY4741 ∆yno1 in untreated controls as well as after addition of alpha factor (100 µg/mL) (N = 8). In (B), differential interference contrast microscopy images of shmoos in the BY4741 and BY4741 ∆yno1 background are shown after addition of alpha factor (100 µg/mL). In (C), the survival rate in the strains BY4741 and BY4741 ∆yno1 was calculated by survival plating. Cell death was induced by addition of alpha factor (100 µg/mL). In untreated cells (BY4741 and BY4741 ∆yno1), no difference in survival was observed. For (A,C): each bar represents the comparison between untreated and treated yeast strains (either BY4741 or BY4741 ∆yno1), asterisks above the bars indicate statistical significance between with/without treatment, asterisks above the brackets represent statistical significance between the different yeast strains (BY4741 and BY4741 ∆yno1) upon treatment with alpha-factor (*: p < 0.1; ***: p < 0.01).
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Figure 6. Expression of YNO1 under conditions that stimulate filamentous growth. The expression of YNO1 was analyzed using the GFP-reporter construct in the strains BY4741 rho+, BY4741 rho0, BY4741 ∆ste20, and BY4741 ∆ste12. Filamentous growth was induced by addition of 0.5% isoamyl alcohol (IAA) for 36 h (A) or growth in galactose media (B) (*: p < 0.1; ***: p < 0.01). In (C), immunoblot analysis of cells harboring a YNO1-GFP fusion (BY4741 YNO1-GFP::HIS3) were grown under the indicated conditions. A control strain (BY4741) was used for the no-GFP control. Equivalently run blots were probed using antibodies to Pgk1 as a loading control. Band intensity of GFP normalized to Pgk1 levels is shown below the blots and was determined by Bio-Rad XRS+ Image Lab software.
Figure 6. Expression of YNO1 under conditions that stimulate filamentous growth. The expression of YNO1 was analyzed using the GFP-reporter construct in the strains BY4741 rho+, BY4741 rho0, BY4741 ∆ste20, and BY4741 ∆ste12. Filamentous growth was induced by addition of 0.5% isoamyl alcohol (IAA) for 36 h (A) or growth in galactose media (B) (*: p < 0.1; ***: p < 0.01). In (C), immunoblot analysis of cells harboring a YNO1-GFP fusion (BY4741 YNO1-GFP::HIS3) were grown under the indicated conditions. A control strain (BY4741) was used for the no-GFP control. Equivalently run blots were probed using antibodies to Pgk1 as a loading control. Band intensity of GFP normalized to Pgk1 levels is shown below the blots and was determined by Bio-Rad XRS+ Image Lab software.
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Figure 7. Evaluating the role of Yno1p in regulating filamentous growth. (A) Wild type cells (PC538), the ∆yno1 mutant (PC7010), and the ∆ste11 mutant (PC3861) in the ∑1278b strain background were spotted onto YEP-GAL media for 3 days at 30 °C. The plate was photographed, washed in a stream of water, and photographed again. (B) Wild type Σ1278b cells (PC538) harboring pYES2 or pYES2-YNO1 plasmids were grown on SC-Ura to maintain selection for the plasmids and spotted onto YEP-GAL media and incubated for 4 days at 30 °C. The plate was photographed, washed in a stream of water and photographed again. (C) Wild type cells and the ∆yno1 mutant either harboring p416GPD or p416GPD-LAS17 plasmids after selection on SC-Ura were spotted onto YEP-GAL media incubated for 4 days at 30 °C. The plate wash assays (PWA) in (A–C) was repeated in multiple biological replicates and invasive growth was quantified using ImageJ. In (A), wild type invasion was set to a value of 1.0, which varied by ± 0.05 across trials. The invasive growth of the ∆yno1 mutant was 0.55 ± 0.11, and ∆ste11 was 0.1 ± 0.09 (N = 3, p < 0.03). In (B), wild type invasion was set to a value of 1.0, which varied by ± 0.12 across trials. The invasion of pYES-YNO1 was 1.42 ± 0.14 (N = 3, p < 0.01). In (C), the intensity of the spots after the PWA are indistinguishable between the wild type p416GPD and ∆yno1 p416GPD-LAS17 cells and are reduced to 0.8 +/– 0.1 in the ∆yno1 p416GPD mutant (N = 4; p = 0. p = 0.05). (D) Wild type cells (PC538) and the ∆yno1 mutant (PC7010) were grown to mid-log phase in YEPD, YEP-GAL, or S-GAL media and examined by differential interference contrast (DIC) microscopy at 100X magnification. Bar, 10 microns. (E) Analysis of cells overexpressing YNO1 by the single cell invasive growth assay (Cullen and Sprague, 2000). Wild type cells (PC538) cells harboring pYES2 or pYES2-YNO1 were grown to saturation in liquid culture and spread onto SC-Ura medium. A spot of galactose (10 microliters of 20% galactose) was spotted onto the plate, which was incubated for 16 h at 30 °C. Cells were examined by DIC microscopy on plates at 100X. Bar, 10 microns. Arrows mark several examples of hyper-polarized cells.
Figure 7. Evaluating the role of Yno1p in regulating filamentous growth. (A) Wild type cells (PC538), the ∆yno1 mutant (PC7010), and the ∆ste11 mutant (PC3861) in the ∑1278b strain background were spotted onto YEP-GAL media for 3 days at 30 °C. The plate was photographed, washed in a stream of water, and photographed again. (B) Wild type Σ1278b cells (PC538) harboring pYES2 or pYES2-YNO1 plasmids were grown on SC-Ura to maintain selection for the plasmids and spotted onto YEP-GAL media and incubated for 4 days at 30 °C. The plate was photographed, washed in a stream of water and photographed again. (C) Wild type cells and the ∆yno1 mutant either harboring p416GPD or p416GPD-LAS17 plasmids after selection on SC-Ura were spotted onto YEP-GAL media incubated for 4 days at 30 °C. The plate wash assays (PWA) in (A–C) was repeated in multiple biological replicates and invasive growth was quantified using ImageJ. In (A), wild type invasion was set to a value of 1.0, which varied by ± 0.05 across trials. The invasive growth of the ∆yno1 mutant was 0.55 ± 0.11, and ∆ste11 was 0.1 ± 0.09 (N = 3, p < 0.03). In (B), wild type invasion was set to a value of 1.0, which varied by ± 0.12 across trials. The invasion of pYES-YNO1 was 1.42 ± 0.14 (N = 3, p < 0.01). In (C), the intensity of the spots after the PWA are indistinguishable between the wild type p416GPD and ∆yno1 p416GPD-LAS17 cells and are reduced to 0.8 +/– 0.1 in the ∆yno1 p416GPD mutant (N = 4; p = 0. p = 0.05). (D) Wild type cells (PC538) and the ∆yno1 mutant (PC7010) were grown to mid-log phase in YEPD, YEP-GAL, or S-GAL media and examined by differential interference contrast (DIC) microscopy at 100X magnification. Bar, 10 microns. (E) Analysis of cells overexpressing YNO1 by the single cell invasive growth assay (Cullen and Sprague, 2000). Wild type cells (PC538) cells harboring pYES2 or pYES2-YNO1 were grown to saturation in liquid culture and spread onto SC-Ura medium. A spot of galactose (10 microliters of 20% galactose) was spotted onto the plate, which was incubated for 16 h at 30 °C. Cells were examined by DIC microscopy on plates at 100X. Bar, 10 microns. Arrows mark several examples of hyper-polarized cells.
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Figure 8. The effect of hydrogen peroxide on invasive growth of the ∆yno1 mutant alongside control strains. Wild type cells (PC538), the ∆yno1 mutant (PC7010) and the ∆ste11 mutant (PC3861) in the ∑1278b strain background were spotted onto YEP-GAL media containing the indicated concentrations of H2O2 for 3 days at 30 °C. Plates were photographed, washed, and photographed again. Invasive growth was quantified by ImageJ analysis.
Figure 8. The effect of hydrogen peroxide on invasive growth of the ∆yno1 mutant alongside control strains. Wild type cells (PC538), the ∆yno1 mutant (PC7010) and the ∆ste11 mutant (PC3861) in the ∑1278b strain background were spotted onto YEP-GAL media containing the indicated concentrations of H2O2 for 3 days at 30 °C. Plates were photographed, washed, and photographed again. Invasive growth was quantified by ImageJ analysis.
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Figure 9. The effect of Yno1p on F-actin distribution at the cell cortex. F-actin structures were visualized by expression from an Abp140-mRFP fusion protein that is integrated into the chromosome. The strains CRY 2845 (MATa SY3089 ste4 FUS1-lacZ FUS1-HIS3 ura3-52; pYES ABP140-RFP-T rho0), CRY 2846 (MATa SY3089 ste4 FUS1-lacZ FUS1-HIS3 ura3-52; pYES YNO1 ABP140-RFP-T rho0), and CRY 2848 (MATa SY3089 ste4 FUS1-lacZ FUS1-HIS3 ura3-52; yno1::KIURA3 ABP140-RFP-T rho-) were grown in synthetic SC-Ura medium and analyzed after addition of 3% galactose for 7 h. Dynamics of F-actin structures was expressed as a T-stack (superposition of 15 images of the cortical F-actin taken in 5 sec intervals). For each strain at least 3 biological and more than 100 cells were inspected. In the strain CRY2845 small actin patches were observed, whereas in the strain CRY2848 there was a prevalence for cortical actin cables. In strain CRY2846, static F-actin bodies of large size are frequently formed (arrow). Bar, 5 μm.
Figure 9. The effect of Yno1p on F-actin distribution at the cell cortex. F-actin structures were visualized by expression from an Abp140-mRFP fusion protein that is integrated into the chromosome. The strains CRY 2845 (MATa SY3089 ste4 FUS1-lacZ FUS1-HIS3 ura3-52; pYES ABP140-RFP-T rho0), CRY 2846 (MATa SY3089 ste4 FUS1-lacZ FUS1-HIS3 ura3-52; pYES YNO1 ABP140-RFP-T rho0), and CRY 2848 (MATa SY3089 ste4 FUS1-lacZ FUS1-HIS3 ura3-52; yno1::KIURA3 ABP140-RFP-T rho-) were grown in synthetic SC-Ura medium and analyzed after addition of 3% galactose for 7 h. Dynamics of F-actin structures was expressed as a T-stack (superposition of 15 images of the cortical F-actin taken in 5 sec intervals). For each strain at least 3 biological and more than 100 cells were inspected. In the strain CRY2845 small actin patches were observed, whereas in the strain CRY2848 there was a prevalence for cortical actin cables. In strain CRY2846, static F-actin bodies of large size are frequently formed (arrow). Bar, 5 μm.
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Figure 10. Model for the action Yno1p during filamentous growth. Galactose induces filamentous growth. The transcription factors Ste12p and Msn2p are activated via Cdc42p/Ste20p and promotes the expression of the Yno1p gene. IAA treatment induces filamentous growth and YNO1 expression independent of Ste12p. Yno1p in an interplay with Sod1p produces hydrogen peroxide that is involved in pheromone response (blue letters), osmotic stress response (red letters) and filamentous growth (green letters). An additional target of Yno1p-derived ROS is the actin assembly factor Las17p that promotes the nucleation of F-actin (purple letters). Our data indicate that stabilized F-actin may impact filamentous/invasive growth.
Figure 10. Model for the action Yno1p during filamentous growth. Galactose induces filamentous growth. The transcription factors Ste12p and Msn2p are activated via Cdc42p/Ste20p and promotes the expression of the Yno1p gene. IAA treatment induces filamentous growth and YNO1 expression independent of Ste12p. Yno1p in an interplay with Sod1p produces hydrogen peroxide that is involved in pheromone response (blue letters), osmotic stress response (red letters) and filamentous growth (green letters). An additional target of Yno1p-derived ROS is the actin assembly factor Las17p that promotes the nucleation of F-actin (purple letters). Our data indicate that stabilized F-actin may impact filamentous/invasive growth.
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Weber, M.; Basu, S.; González, B.; Greslehner, G.P.; Singer, S.; Haskova, D.; Hasek, J.; Breitenbach, M.; W.Gourlay, C.; Cullen, P.J.; et al. Actin Cytoskeleton Regulation by the Yeast NADPH Oxidase Yno1p Impacts Processes Controlled by MAPK Pathways. Antioxidants 2021, 10, 322. https://0-doi-org.brum.beds.ac.uk/10.3390/antiox10020322

AMA Style

Weber M, Basu S, González B, Greslehner GP, Singer S, Haskova D, Hasek J, Breitenbach M, W.Gourlay C, Cullen PJ, et al. Actin Cytoskeleton Regulation by the Yeast NADPH Oxidase Yno1p Impacts Processes Controlled by MAPK Pathways. Antioxidants. 2021; 10(2):322. https://0-doi-org.brum.beds.ac.uk/10.3390/antiox10020322

Chicago/Turabian Style

Weber, Manuela, Sukanya Basu, Beatriz González, Gregor P. Greslehner, Stefanie Singer, Danusa Haskova, Jiri Hasek, Michael Breitenbach, Campbell W.Gourlay, Paul J. Cullen, and et al. 2021. "Actin Cytoskeleton Regulation by the Yeast NADPH Oxidase Yno1p Impacts Processes Controlled by MAPK Pathways" Antioxidants 10, no. 2: 322. https://0-doi-org.brum.beds.ac.uk/10.3390/antiox10020322

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