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Article

Effects of Rht-B1b and Ddw1 Dwarfing Genes in Two Connecting Populations of Spring Triticale under Greenhouse Experiment Conditions

1
Laboratory of Applied Genomics and Crop Breeding, All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya str. 42, Moscow 127550, Russia
2
Centre for Molecular Biotechnology, Russian State Agrarian University–Moscow Timiryazev Agricultural Academy, Timiryazevskaya street, 49, Moscow 127550, Russia
3
Laboratory of Marker-Assisted and Genomic Selection of Plants, All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya str. 42, Moscow 127550, Russia
4
Department of Genetics, Biotechnology, Plant Breeding and Seed Science, Russian State Agrarian University–Moscow Timiryazev Agricultural Academy, Timiryazevskaya street, 49, Moscow 127550, Russia
*
Author to whom correspondence should be addressed.
Submission received: 5 May 2019 / Revised: 3 June 2019 / Accepted: 5 June 2019 / Published: 7 June 2019

Abstract

:
Dwarfing genes not only reduce the height of triticale plants, but also have pleiotropic effects on important agronomic traits. An important task for breeding is to evaluate the effects of gibberellin responsive (GAR) and gibberellin-insensitive (GAI) dwarfing genes in one genotype. In the greenhouse experiment, we evaluated the effects of the GAI gene Rht-B1b of wheat and the GAR gene Ddw1 of rye on height and the main agronomic traits in two connecting populations derived from crossing Ddw1 donors (cv. ‘Mudrets’ and cv. ‘Valentin 90’) with a Rht-B1b donor (cv. ‘Dublet’). The results show a strong decrease in height under the influence of Ddw1 in both populations by more than 30%. In this case, Rht-B1b in the presence of Ddw1 does not lead to a significant decrease in the height of the spring triticale; thus, this is not likely to be included in breeding programs in order to further reduce the height in the presence of Ddw1 in the spring triticale germplasm. However, Ddw1 reduces the 1000 grain weight, while Rht-B1b increases the grain number per spike and grain number per spikelet. Thus, our studies have demonstrated the negative effect of Ddw1 on spring triticale productivity of the main spike in the greenhouse experiment, which can be partially compensated by Rht-B1b.

1. Introduction

Triticale plays an increasingly important role in agriculture. Products from this crop are in demand in the livestock sector as feed, in the alcohol-production industry, and have good prospects in the baking industry. Due to its high ecological plasticity, triticale is capable of producing stable yields under unstable agriculture. Triticale is grown in Europe, and among the leaders of its production are Germany, Poland, Belarus, France, and Russia [1].
One of the strategies to increase the productivity of triticale may be optimization of the plant architecture defined by tillering, stature, and spike morphology [2]. Plant architecture is one of the most important agronomic traits that determines the adaptability of a plant for cultivation, the yield index, and the potential grain yield [3]. One of the important components of plant architecture is height [2]. Since triticale combines the genetic material of two species, wheat and rye, it is possible to influence plant height by introducing dwarfing genes from both the wheat and rye genomes. In general, dwarfing genes decreasing the height of the stem lead to the redistribution of assimilants from stem to spike, which allows more flowers to form and survive in the spike [4,5,6,7]. Short-stem (dwarf and semi-dwarf) forms have a higher harvest index both due to a decrease in aboveground biomass and due to an increase in the number of grains. Finally, crop losses in lodging-resistant forms of wheat are lower. Dwarf plants are considered to be more responsive to favorable cultivation conditions, while tall forms have an advantage in dry and hot weather conditions [8]. Dwarfing genes of wheat and rye origin are divided into two classes according to their response to exogenous gibberellic acid (GA): GAI gibberellin insensitive and GAR gibberellin responsive. To date, 24 genes that reduce plant height have been identified in bread wheat [9,10]. The gibberellin-insensitive gene Rht-B1b is one of the genes successfully introduced as a result of the Green Revolution and is widely distributed throughout the world [11]. The effect of the gibberellin-insensitive gene Rht-B1b on height can vary from about 10 to 25% in bread wheat and from about 25 to 35% in durum wheat compared to the wild-type allele Rht-B1a [6,12,13,14,15,16,17]. Wheat varieties and lines with Rht-B1b compared to Rht-B1a show a higher grain yield compared to wild type [6,15,16,17,18]. Rht-B1b increases the number of grains in the spike [17,18] and, as a result, the number of grains per square meter [15,16,17]. A number of studies have shown that Rht-B1b reduces the average mass of a single grain, although in a number of experiments the effect was not significant [12,13,15,16,17]. Also, an increase in harvest index due to an increase in grain yield and a decrease in the total aboveground biomass is shown [15,17]. Reductions of flag leaf area under the influence of Rht-B1b have not been shown [16,17]. Thus, Rht-B1b in wheat increases the yield by increasing the number of grains in the spike and per square meter, although the grain becomes smaller.
In rye, plant height is also determined by a variety of genetic factors [19,20]. To date, 14 different rye dwarfing genes are known, of which the GAR gibberellin-responsive dominant Ddw1 gene, first identified in the rye EM-1 mutant form, is of the greatest breeding value [21,22]. Unlike GAI gibberellin-insensitive genes, GAR gibberellin-responsive genes do not reduce the length of the coleoptile, allowing deep seeding under the conditions of insufficient soil moistening. The introgression of Ddw1 in winter triticale led to a decrease in height, but the resulting forms were later in development, reducing the linear dimensions of the leaves, reducing grain yield and resistance to Fusarium head blight [23,24,25].
Assessment of the interaction effects of GAR gibberellin-responsive and GAI gibberellin-insensitive dwarfing genes is important for selecting the most optimal combination of them in the genome of spring triticale and obtaining the optimum plant height for a particular growing area. The combination of genes with different mechanisms of action can enhance the positive effects of various dwarfing genes [16,17]. It should be taken into consideration that their effects on rye and wheat may differ from those in triticale, where they interact with each other in one plant organism combining the genetic environment of two different species. The goal of our study is to evaluate the effects of Rht-B1b and Ddw1 on height and a number of important agronomic traits in two connected populations of spring triticale in F2 under a greenhouse conditions experiment.

2. Materials and Methods

2.1. Plant Material

As parental forms, triticale cultivars bearing contrasting combinations of rye and wheat dwarfing alleles were chosen. Spring triticale cv. ‘Dublet’ with genotype ddw1 ddw1 Rht-B1b Rht-B1b (Danko Hodowla Roślin Sp. z o.o., Poland) was used as the paternal form and winter triticale cv. ‘Mudrets’ (M) and cv. ‘Valentin 90’ (V90) with genotype Ddw1 Ddw1 Rht-B1a Rht-B1a (Lukyanenko Agricultural Research Institute, Russia) were used as the maternal forms.

2.2. Hybridization

The seeds of the parental cultivars were sown into the vegetation pots at 10 seeds per pot in the greenhouse of the Center for Molecular Biotechnology (Russian State Agrarian University—Moscow Timiryazev Agricultural Academy). For vernalization, the maternal plants at tillering were placed into a spring chamber for 60 days and then returned to the greenhouse. When maternal plants were heading the anthers in their spike were removed manually with fine forceps, the spikes were isolated with butter paper bags. The maternal plants were pollinated by placing a spike of a cut flowering paternal plant under a paper bag. The cut stem of the paternal plant was placed in water to keep it alive as long as possible.

2.3. Growth Conditions

Growing F1 and F2 plants was carried out in the same greenhouse as the parental plants under the same lighting conditions with five plants per pot, provided with watering to maintain 60–70% soil moisture and the single application of 2 g of fertilizers per pot at the tillering stage (N 16%, P2O5 16%, K2O 16%). Since the maternal form in both combinations was winter, in the segregating F2 population there was a certain amount of winter forms, which we selected out according to the phenotype. A total of 318 plants of the ‘Mudrets’ × ‘Dublet’ (M×D) and 407 plants of the ‘Valentin 90’ × ‘Dublet’ (V90×D) F2 segregating populations were analyzed.

2.4. Phenotyping

After full ripening and harvesting, a structural analysis of each individual plant was carried out according to the following phenotypic characters: plant height (cm), length of peduncle (first upper internode), 2nd and 3rd upper internodes, 2nd and 1st lower internodes (cm); main spike length (cm), spikelet number per main spike, main spike density (calculated as tenfold spikelet number per main spike divided by main spike length), grain weight per main spike (g), grain number per main spike, grain number per spikelet (grain number per main spike divided by spikelet number per main spike), 1000-grain weight (calculated as thousandfold grain weight per main spike divided by grain number per main spike, g), number of fertile tillers; and number of internodes. Additionally, heading date (days after sowing) and flowering date (days after sowing) were registered in growing plants.

2.5. Molecular analysis

DNA was extracted from each individual plant in F1 and F2 generations at tillering, and the allele status of the Rht-B1 and Ddw1 genes was determined using molecular markers. Genomic DNA was isolated from individual vegetative plants tissue using the CTAB method [26]. Primers BF, MR1 and WR1 were used to identify the Rht-B1a (wild type) and Rht-B1b (dwarfing type) alleles; PCR was performed in accordance with the conditions recommended by the authors of the molecular marker [27] (Table 1). The PCR products were separated in 2% agarose gel in TBE buffer and stained with ethidium bromide for subsequent visualization in a UV transilluminator. The presence of Ddw1 was determined using primers developed on the sequence of the microsatellite locus REMS1218, closely linked to the gene [28] (Table 1). The determination of REMS1218 microsatellite alleles was carried out using PCR according to the protocol proposed by the authors of the molecular marker and subsequent fragment analysis using 3130xl Genetic Analyzer (Applied Biosystems, USA).

2.6. Statistical Analysis

For each phenotypic trait, the mean, standard deviation and range were determined. All data were processed in the Statistica 8.0 (StatSoft) program by regression and analysis of variance (ANOVA); to determine the significance of the differences between the mean values we used the least significance difference (LSD) criterion at a 95% confidence level. When analyzing the interaction of Rht-B1b and Ddw1, the effect of each of the alleles and their total effect were taken into account as follows:
Effect   of   R h t B 1 b   ( % ) = Mean ( 02 ) Mean ( 00 ) Mean   ( 00 ) 100 ,
Effect   of   D d w 1 ( % ) = Mean ( 20 ) Mean ( 00 ) Mean   ( 00 ) 100 ,
Effect   of   R h t B 1 b + D d w 1   ( % ) = Mean ( 22 ) Mean ( 00 ) Mean   ( 00 ) 100 .
where.
  • 00 – ddw1 ddw1 Rht-B1a Rht-B1a (absence of both dwarfing genes, wild type, tall plant),
  • 02 – ddw1 ddw1 Rht-B1b Rht-B1b (the presence of only one dwarfing gene Rht-B1b from wheat),
  • 20 – Ddw1 Ddw1 Rht-B1a Rht-B1a (the presence of only one dwarfing gene Ddw1 from rye),
  • 22 – Ddw1 Ddw1 Rht-B1b Rht-B1b (the presence of both dwarfing genes)
Fractional ratios of internodes (%) were calculated as the length of a given internode divided by the total plant height expressed in percent.

3. Results

3.1. Molecular Analysis

Each plant of F1 and F2 generations in Mudrets’ × ‘Dublet’ (M×D) and ‘Valentin 90’ × ‘Dublet’ (V90×D) populations were genotyped at tillering. The allelic status of RhtB1 (Supplementary Figure S1) and Ddw1 (Supplementary Figure S2) was detected using PCR molecular analysis. Based on the results of molecular analysis, the F2 plants were categorized into four groups according to the presence or absence of dwarfing alleles, namely ddw1 ddw1 Rht-B1a Rht-B1a (00), ddw1 ddw1 Rht-B1b Rht-B1b (02), Ddw1 Ddw1 Rht-B1a Rht-B1a (20), and Ddw1 Ddw1 Rht-B1b Rht-B1b (22).

3.2. Plant Height.

The average plant height without dwarfing genes (genotype 00) differed in two populations by almost 15 cm, 104.7 cm in ‘Mudrets’ × ‘Dublet’ (M×D) and 118.7 cm in ‘Valentin 90’ × ‘Dublet’ (V90×D) (Table 2, Supplementary Table S1). Apparently, this is the reason for the absence of a statistically significant effect of Rht-B1b on height in the M×D population, although, in general, plants with genotype 02 (that is, only with Rht-B1b) were slightly lower than plants with genotype 00. In the V90xD population, the effect of Rht-B1b had a significant effect and reduced plant height by 11.2 cm (9.4%). At the same time, Ddw1 significantly reduced height by 38.9 cm (37.2%) in M×D, and by 35.8 cm (30.2%) in V90×D. When both dwarfing genes were present, no additional effect on the decrease in height was found. Thus, Rht-B1b does not reduce the height of plants additionally to the effect of Ddw1. The effect of Ddw1 is manifested at each internode in both populations; the effect of Rht-B1b is statistically significant only at the two lower internodes (Figure 1, Supplementary Table S1). Although there is some tendency to reduce the length of each individual internode, the additional presence of Rht-B1b in the presence of Ddw1 does not give a statistically significant effect, as in the case of height. It should be noted that there is a decrease in the number of internodes in the case of the presence of only Ddw1 in the M×D population. In both populations, under the influence of both Ddw1 and Rht-B1b genes, there is a proportional decrease in internodes (Table 3). Thus, the ratio of the internode length to the height of the plant is maintained on average within the following limits: 33% (peduncle): 22% (2nd upper internode); 15–16% (3rd upper internode); 12% (2nd lower internode); and 5% (1st lower internode). Thus, we can say that the ratio between the internodes is a rather conservative factor, and the Ddw1 and Rht-B1b alleles do not have a significant effect on this indicator.

3.3. The Structure and Productivity of the Main Spike

In general, the presence of Rht-B1b did not affect the main spike length in both populations. The presence of Ddw1 somewhat reduced the spike length. This was statistically significant only in genotype 02 (Ddw1 Ddw1 Rht-B1a Rht-B1a) in the V90×D population (Supplementary Table S1). None of the studied dwarfing genes had a statistically significant effect on the spikelet number per main spike in any of the populations (Supplementary Table S1). In the M×D population, the main spike density in plants with the genotype 22 (both dwarfing genes) was found to be 6.8 units (33.6%) more than that of 00 (absence of both dwarfing genes) and 7.9 units (40.9%) more than 02 (ddw1 ddw1 Rht-B1b Rht-B1b). In the V90×D population, in plants with the 20 genotype, the spike density is 11.3 units (45.8%) more than 00 genotype, and 10.2 units (39.5%) more than 02 genotype (Supplementary Table S1). Thus, in both populations, an increase in the spike density occurs because of a decrease in the spike length in the M×D population due to the interaction of the Ddw1 and Rht-B1b alleles, and in the V90×D population only due to Ddw1.
When analyzing the influence of two dwarfing alleles on the grain weight per main spike, it was found that in the M×D population a significant difference from the 00 genotype (ddw1 ddw1 Rht-B1a Rht-B1a) has only plants with 20 genotype (Ddw1 Ddw1 Rht-B1a Rht-B1a ) with its grain weight per spike less by 0.52 g (−31.5%) (Supplementary Table S1). No significant differences in the grain weight per spike between homozygotes in the V90×D population were found. We conducted a statistical analysis of the effects of Rht-B1b without taking into account the presence/absence of Ddw1 and the effects of Ddw1 without taking into account the presence/absence of Rht-B1b (independent analysis, Supplementary Table S2). An independent analysis demonstrated that Ddw1 reduces the grain weight per spike in both populations; Rht-B1b increases the grain weight per spike in the M×D population.
The effect of dwarfing genes on the grain number per main spike in both populations showed similar trends: In the group of plants with genotype 02 (with Rht-B1b), the grain number per spike was slightly higher, and in the group of plants with genotype 20 (with Ddw1), it was slightly lower than in the wild type (genotype 00) (Table 2, Supplementary Table S1). At the same time, the presence of both Rht-B1b and Ddw1 in different populations gave a different effect: In the M×D population, the grain number per spike of was at the level of genotype 02 (with Rht-B1b) and in the population of V90×D at the level of genotype 00 (absence of both dwarfing genes). These differences were statistically insignificant, so we conducted an independent statistical analysis of the effects of Rht-B1b and Ddw1 (Supplementary Table S2). An independent analysis showed that Ddw1 in both populations does not affect the grain number per spike. It was also demonstrated that Rht-B1b significantly increases the grain number per spike in the M×D population by 0.29 pcs (18.96%). In the population of V90×D, the significant effect of Rht-B1b is not observed; however, the difference between homozygotes for Rht-B1 is close to the critical value of 4.7 g (10.1%) with LSD0.05 = 5.2. This effect is confirmed by the significant weak correlation found using the regression analysis (0.20) and the significant Spearman’s coefficient (0.15).
In the M×D population, plants with the 22 genotype (Ddw1 Ddw1 Rht-B1b Rht-B1b) have a grain number per spikelet of 0.37 units (27.0%) more than plants with the 20 genotype (Ddw1 Ddw1 Rht-B1a Rht-B1a), and plants with the 02 genotype (ddw1 ddw1 Rht-B1b Rht-B1b) have a grain number per spikelet of 0.49 units (26.3%) more than the 20 genotype (Ddw1 Ddw1 Rht-B1a Rht-B1a) (Supplementary Table S1). In the V90×D population, none of the dwarfing alleles showed a statistically significant effect on the grain number per spikelet, but we have noted the tendency that plants carrying the dwarfing gene Rht-B1b have a somewhat greater grain number per spikelet. Thus, the presence of the Rht-B1b allele in both populations increases the grain number per spikelet, while we have not revealed the statistical effect of Ddw1, although there is a tendency of decreasing this trait by Ddw1 (Supplementary Table S1).
The 1000 grain weight decreased in both populations under the influence of Ddw1 (Supplementary Table S1; Table 2). In the M×D population, the presence of Ddw1 reduces the 1000 grain weight by 9.9 g (21.5%) and together with Ddw1 with Rht-B1b by 8.4 g (18.2%). At the same time, the 1000 grain weight in plants with genotypes 20 (Ddw1 Ddw1 Rht-B1a Rht-B1a) and 22 (Ddw1 Ddw1 Rht-B1b Rht-B1b) is not significantly different. In the V90×D population, a statistically significant decrease in the 1000 grain weight is observed only with the simultaneous presence of Ddw1 and Rht-B1b; the decrease relative to the 00 genotype (ddw1 ddw1 Rht-B1a Rht-B1a) is 8.5 g (19.4%). Thus, in both populations, Ddw1 reduces the 1000 grain weight (Table 2, Supplementary Table S1).
The influence of dwarfing genes on tillering capacity was not found in both populations (Supplementary Table S1).

3.4. Flowering and Heading.

The 20 homozygotes (Ddw1 Ddw1 Rht-B1a Rht-B1a) came into heading 5.3 days later than the 00 homozygotes (ddw1 ddw1 Rht-B1a Rht-B1a) in the M×D population. In the V90×D population, none of the dwarfing alleles had a statistically significant effect on heading or flowering (Table 2, Supplementary Table S1).

4. Discussion and Conclusions

The task of the breeder is primarily to increase the adaptability of the plant to the environment, that is, in the model of the variety (ideotype), the key factor is the height and flowering period, which most closely matches the environment [16]. Dwarfing genes affect not only the height, but also the significant agronomic traits associated with it. A tall stem accumulates more assimilants than a short one; therefore, during the remobilization, tall plants form a larger and more filled grain. In addition, dwarf plants are formed due to the slower elongation of the stem, which leads to a later heading and flowering, and consequently, to lower yields [12]. Thus, the selection of dwarfing genes that would provide optimal flowering and heading periods and stem length for specific growing conditions is an important and critical task.
We analyzed the effect of Ddw1 and Rht-B1b on the height of spring triticale plants in two connected populations. This provided an assessment of the manifestation of these genes in a different genetic background. This shows that Ddw1 reduces the height of spring triticale plants in both by approximately 30%. In winter triticale, this value was on average about 20 cm (20–25%) [25,30], and in diploid rye, the reduction can reach 40% [21,29]. In our experiment, Rht-B1b in the M×D population did not have a statistically significant effect on height, which may be due to the strong influence of the genetic environment on plant height. In the V90×D population, Rht-B1b reduced height only in the absence of Ddw1 (about 9%). In this case, Rht-B1b did not give an additional effect of reducing the height with Ddw1. The absence of the effect of Rht-B1b with Ddw1 can be explained by the strong masking effect of Ddw1, in which a slight decrease in height due to Rht-B1b is not noticeable. The reduction in the length of internodes due to Ddw1 occurs proportionally, that means the ratio of internodes between themselves is maintained. This is not typical for all GAR gibberellin responsive alleles: Rht-12 (probable ortholog of Ddw1) disproportionately changes the peduncle length.
Ddw1 in the population ‘Mudrets’ × ‘Dublet’ significantly reduced the spike length only in the presence of Rht-B1b, and in the population of ‘Valentin 90’ × ‘Dublet’ in its absence, while the decrease in both cases was about 20%. According to Kobyliansky and Solodukhina (2015), in rye, under the influence of Ddw1, on the contrary, an increase in the spike length occurs [21]. Rht-B1b reduces the sensitivity of tissues to the GA, which leads to less cell stretching; Ddw1 has a different mechanism of action. Probably, under the influence of the surrounding genetic background, there is a different interaction of these mechanisms in different populations. In both populations, the grain weight per spike was reduced by 12–18% due to Ddw1; the number of grains was not affected by Ddw1, and the weight of 1000 grains decreased by 12–13%. Rebetzke et al. (2012) showed that, under the influence of Rht12 (a possible Ddw1 ortholog of wheat), there is a decrease in the mass of one grain by about 12%, which is comparable with our data [16]. Kobyliansky and Solodukhina (2015) showed that the number of grains in the spike increases in rye under the influence of Ddw1, which is not observed in our experiments [21]. The effect of Rht-B1b on the grain weight and the amount of grains in the spike of common wheat greatly depends on the test conditions and the genetic environment and can be both positive and negative [18]. The 1000 grain weight (or the average grain weight) in bread wheat under the influence of Rht-B1b may decrease significantly or may not statistically significantly differ from Rht-B1a depending on the genetic environment and experimental conditions. [12,13,15,16]. Our experiment also shows the dependence of the Rht-B1b effect on the surrounding genotype. In the M×D population, Rht-B1b eliminates the negative effect of Ddw1 in relation to the grain weight per spike due to an increase in the grain number per spike and spikelet and does not significantly reduce the 1000 grain weight. In the V90×D population, Rht-B1b, on the other hand, enhances the effect of Ddw1 on reducing the 1000 grain weight. The presence of Ddw1 led to a later heading and flowering in the M×D population (Supplementary Table S2); no such effect was observed in the V90×D population. Kalih et al. (2015) [31] and Wolski et al. (1996) [23] also wrote that Ddw1 leads to later periods of flowering and heading in triticale. Later flowering and heading may be the result of a smaller grain weight, as is the case, for example, for the GAR gibberellin-responsive Rht-5 wheat gene [32,33,34].
Thus, our studies show the negative effect of Ddw1 in spring triticale on the productivity of the main spike in the greenhouse experiment, which can be partially compensated by Rht-B1b. There is no positive effect of Ddw1 on the number of grains, as it happens in rye, but we have observed that the gene at least does not reduce their number. A decrease in the 1000 grain weight under the influence of Ddw1 was expected. Rht-B1b increased the grain number per main spike and the grain number per spikelet, which may be due to faster delivery of assimilants to the spike, greater survival of distant spikelets, and increased fertility. In general, we found that the effect of Ddw1 and Rht-B1b on height and agronomic characters depends on the population.
In triticale, combining dwarfing GAR and GAI genes from wheat and rye genomes increases the possible variations in their effects and enables the search for more appropriate variants. The combination of GAR and GAI dwarfing genes is an important direction in breeding of short-stem cereals as it provides an opportunity to achieve the compensating or/and additive effects. For example, Kurkiev (2008) [35] demonstrated additive effects on reducing plant height in triticale plants with both 2R/2D substitutions and rye and wheat dwarfing genes; Rebetzke et al. (2012) [15] and Wang et al. (2014) [36] showed that the plants of wheat having both GAI and GAR dwarfing genes was shorter than that with a single of the studied genes, whereas Liu et al. (2017) [17] in wheat, and we in our study of triticale did not find additive effects between GAR and GAI genes on plant height. In wheat, the combination of GAR and GAI genes does not additively reduce 1000 grain weight (or average single kernel weight) even if GAR alone severely reduces it [17,36]. In our study, the interaction of Rht-B1b and Ddw1 resulted in a significant drop in the 1000 grain weight in the V90×D population while there was no additional reduction of this trait in GAR+GAI plants in the M×D population. In wheat, the grain number per spike in lines with the combination of the GAR and GAI gene was less than in lines with the single dwarfing genes [35]; in our study, in the V90×D population, the grain number per spike was significantly higher in plants with the combination of the GAR and GAI genes than in plants with the GAR Ddw1 gene; this is an example of the compensating effect between the genes.
In summary, in the studied spring triticale populations, Ddw1 was found to decrease plant height with the greatest contribution of peduncle, to decrease the grain weight per main spike and the 1000 grain weight, while Rht-B1b is associated with an increase in the grain number per main spike. Individual effects may be due to a specific set of minor genes in each population that affect the studied traits.

Supplementary Materials

The following are available online at https://0-www-mdpi-com.brum.beds.ac.uk/2077-0472/9/6/119/s1, Figure S1: PCR analysis of F2 plants for the allelic state of Rht-B1 using the marker by Ellis et al., 2002 [27], Figure S2: Fragment analysis of F2 plants for the allelic state of Ddw1 using the marker by Tenhola-Roininen et al., 2010 [29], Table S1: Effects of Ddw1 and Rht-B1b in the spring triticale population F2 ‘Mudrets’ × ‘Dublet’ and × ‘Valentin 90’ × ‘Dublet’ in the greenhouse experiment, Table S2: Independent effects of Ddw1 and Rht-B1b in the spring triticale population F2 ‘Mudrets’ × ‘Dublet’ and × ‘Valentin 90’ × ‘Dublet’ in the greenhouse experiment.

Author Contributions

Conceptualization, M.D.; Methodology, M.D.; Formal analysis, P.K., A.K, V.R., V.I. and A.Ch.; Investigation, M.D. and P.K.; Resources, A.S. and G.K.; Writing—original draft preparation, P.K.; Writing—review and editing, M.D. and G.K.; Funding acquisition, M.D.

Funding

This research was funded by Russian Science Foundation, grant number 17-76-20023.

Conflicts of Interest

The authors declare no conflict of interest.

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Figure 1. The effects of Ddw1 and Rht-B1b on length distribution across main internodes in ‘Mudrets’ × ‘Dublet’ (M×D) and ‘Valentin 90’ × ‘Dublet’ (V90×D) spring triticale. From top to bottom: main spike, peduncle (1st upper) internode, 2nd upper internode, 3rd upper internode; 2nd lower internode; 1st lower internode; vertical axis: 00, ddw1 ddw1 Rht-B1a Rht-B1a; 02, ddw1 ddw1 Rht-B1b Rht-B1b; 20, Ddw1 Ddw1 Rht-B1a Rht-B1a; 22, Ddw1 Ddw1 Rht-B1b Rht-B1b.
Figure 1. The effects of Ddw1 and Rht-B1b on length distribution across main internodes in ‘Mudrets’ × ‘Dublet’ (M×D) and ‘Valentin 90’ × ‘Dublet’ (V90×D) spring triticale. From top to bottom: main spike, peduncle (1st upper) internode, 2nd upper internode, 3rd upper internode; 2nd lower internode; 1st lower internode; vertical axis: 00, ddw1 ddw1 Rht-B1a Rht-B1a; 02, ddw1 ddw1 Rht-B1b Rht-B1b; 20, Ddw1 Ddw1 Rht-B1a Rht-B1a; 22, Ddw1 Ddw1 Rht-B1b Rht-B1b.
Agriculture 09 00119 g001
Table 1. Primers used for the identification of allelic state of the Rht-B1 and Ddw1 genes.
Table 1. Primers used for the identification of allelic state of the Rht-B1 and Ddw1 genes.
GenePrimerSourceExpected Amplicon Size
Rht-B1BF 5′ GGTAGGGAGGCGAGAGGCGAG 3′Ellis et al., 2002 [27]BF+MR1: 237 bp for Rht-B1b
BF+WR1: 237 bp for Rht-B1a
MR1 5′ CATCCCCATGGCCATCTCGAGCTA 3′
WR1 5′ CATCCCCATGGCCATCTCGAGCTG 3′
Ddw1F 5’ GTGCTATGATAAGTACCTGC 3′Tenhola-Roininen et al., 2010 [29]317 bp for ddw1
317+321 bp for Ddw1
R 5’ GGC TAA GGG AAC TCG CATTG 3′
Table 2. Effects of Ddw1 and Rht-B1b on several agronomic traits in the spring triticale population F2 ‘Hongor’ × ‘Dublet’ and × ‘Valentin 90’ × ‘Dublet’ in the greenhouse experiment.
Table 2. Effects of Ddw1 and Rht-B1b on several agronomic traits in the spring triticale population F2 ‘Hongor’ × ‘Dublet’ and × ‘Valentin 90’ × ‘Dublet’ in the greenhouse experiment.
GenotypePlant Height, cmGrain Number per Spike1000-Grain Weight, gHeading Time (Days after Sowing)
Mudrets × DubletValentin 90 × DubletMudrets × DubletValentin 90 × DubletMudrets × DubletValentin 90 × DubletMudrets × DubletValentin 90 × Dublet
00 **104.7 ± 26.0 a118.7 ± 19.1 a35.1 ± 14.7 ab49.3 ± 14.7 a46.0 ± 11.1 a43.6 ± 6.9 a49.1 ± 5.9 a50.9 ± 7.4 a
02100.1 ± 24.5 a107.5 ± 11.7 b40.7 ± 12.7 a53.1 ± 16.4 a42.1 ± 8.8 ab41.1 ± 7.3 a47.0 ± 5.1 a50.8 ± 8.2 a
2065.8 ± 19.6 b82.9 ± 14.7 c31.6 ± 11.7 b44.5 ± 13.1 a36.1 ± 8.4 c39.9 ± 9.6 a54.4 ± 9.8 b52.6 ± 6.8 a
2273.7 ± 13.1 b77.6 ± 10.3 c40.7 ± 10.6 a50.6 ± 17.9 a37.6 ± 8.9 bc35.1 ± 5.7 b51.3 ± 3.7 ab48.9 ± 3.3 a
Contrasts
02vs00−4.6 (−4.4%)−11.2 * (−9.4%)5.6 (15.8%)3.8 (7.8%)−3.9 (−8.5%)−2.5 (−5.8%)−2.1(−4.3%)−0.1 (−0.3%)
LSD0.0512.49.68.510.56.26.14.45.1
20vs00−38.9 * (−37.2%)−35.8 * (−30.2%)−3.5 (−10%)−4.8 (−9.7%)−9.9 * (−21.5%)−3.7 (−8.5%)5.3 * (10.8%)1.7 (3.4%)
LSD0.0513.09.49.010.96.56.24.45.1
22vs00−31.0 * (−29.6%)−41.1 * (−34.6%)5.5 (15.7%)1.3 (2.7%)−8.4 * (−18.2%)−8.5 * (−19.4%)2.2(4.4%)−2.0 (−3.9%)
LSD0.0513.69.79.110.56.66.04.95.4
* Significant differences (p = 0.05) are marked with asterisks (*) and typed in bold; the letters demonstrate if the means are different; ** 00—ddw1 ddw1 Rht-B1a Rht-B1a (absence of both dwarfing genes, wild type, tall plant), 02—ddw1 ddw1 Rht-B1b Rht-B1b (the presence of only one dwarfing gene Rht-B1b), 20—Ddw1 Ddw1 Rht-B1a Rht-B1a (the presence of only one dwarfing gene Ddw1), 22—Ddw1 Ddw1 Rht-B1b Rht-B1b (the presence of both dwarfing genes); LSD0.05, least significant difference at 95% confidence level; SD, standard deviation.
Table 3. Fractional ratios of the internode length in the ‘Mudrets’ × ‘Dublet’ and ‘Valentin 90’ × ‘Dublet’ F2 populations in the greenhouse experiment (%).
Table 3. Fractional ratios of the internode length in the ‘Mudrets’ × ‘Dublet’ and ‘Valentin 90’ × ‘Dublet’ F2 populations in the greenhouse experiment (%).
Internode *Mudrets × DubletValentin 90 × Dublet
Genotype **F0.05GenotypeF0.05
0002202200022022
Peduncle length31.732.734.633.30.6832.335.732.332.21.47
2nd UIN22.722.620.622.90.3022.724.421.822.01.25
3rd UIN15.718.515.815.60.7015.115.415.115.50.96
2nd LIN10.714.411.711.70.5011.511.011.612.40.81
1st LIN4.16.64.85.20.685.94.65.54.61.95
F0.05 (df1 = 4, df2 = 269) = 2.41F0.05 (df1 = 4, df2 = 273) = 2.40
* UIN, upper internode; LIN, low internode; ** genotype designation the same as in Table 2; F0.05, Fisher criterion at 95% confidence level.

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Chernook, A.; Kroupin, P.; Karlov, G.; Soloviev, A.; Korshunova, A.; Rubets, V.; Igonin, V.; Divashuk, M. Effects of Rht-B1b and Ddw1 Dwarfing Genes in Two Connecting Populations of Spring Triticale under Greenhouse Experiment Conditions. Agriculture 2019, 9, 119. https://0-doi-org.brum.beds.ac.uk/10.3390/agriculture9060119

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Chernook A, Kroupin P, Karlov G, Soloviev A, Korshunova A, Rubets V, Igonin V, Divashuk M. Effects of Rht-B1b and Ddw1 Dwarfing Genes in Two Connecting Populations of Spring Triticale under Greenhouse Experiment Conditions. Agriculture. 2019; 9(6):119. https://0-doi-org.brum.beds.ac.uk/10.3390/agriculture9060119

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Chernook, Anastasiya, Pavel Kroupin, Gennady Karlov, Alexander Soloviev, Anastasiya Korshunova, Valentina Rubets, Vladimir Igonin, and Mikhail Divashuk. 2019. "Effects of Rht-B1b and Ddw1 Dwarfing Genes in Two Connecting Populations of Spring Triticale under Greenhouse Experiment Conditions" Agriculture 9, no. 6: 119. https://0-doi-org.brum.beds.ac.uk/10.3390/agriculture9060119

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