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Article

Considerations and Influencing Parameters in EDS Microanalysis of Biogenic Hydroxyapatite

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Faculty of Materials Science and Engineering, University Politehnica of Bucharest, 313 Splaiul Independentei, 011061 Bucharest, Romania
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S.C. Nuclear NDT Research & Services S.R.L, 104 Berceni St., Central Laboratory Building, 041919 Bucharest, Romania
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Department of Analytical Chemistry and Environmental Engineering, University Politehnica of Bucharest, 011061 Bucharest, Romania
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Department of Prosthetics Technology and Dental Materials, University of Medicine and Pharmacy, 020022 Bucharest, Romania
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Author to whom correspondence should be addressed.
J. Funct. Biomater. 2020, 11(4), 82; https://0-doi-org.brum.beds.ac.uk/10.3390/jfb11040082
Received: 23 September 2020 / Revised: 7 November 2020 / Accepted: 11 November 2020 / Published: 15 November 2020
(This article belongs to the Special Issue Bioceramics and Bioactive Glass-Based Materials)
Calcium phosphates (CPs) used as biomaterials have been intensively studied in recent years. In most studies, the determination of the chemical composition is mandatory. Due to the versatility and possibilities of performing qualitative and quantitative compositional analyses, energy dispersive spectrometry (EDS) is a widely used technique in this regard. The range of calcium phosphates is very diverse, the first method of approximating the type of compound being EDS microanalysis, by assessing the atomic Ca/P ratio. The value of this ratio can be influenced by several factors correlated with instrumental parameters and analysed samples. This article highlights the influence of the electron beam acceleration voltage (1 kV–30 kV) and of the particle size of calcium phosphate powders on the EDS analysis results. The characterised powders were obtained from bovine bones heat-treated at 1200 °C for 2 h, which have been ground and granulometrically sorted by mechanical vibration. The granulometric sorting generated three types of samples, with particle sizes < 20 μm, < 40 μm and < 100 μm, respectively. These were morphologically and dimensionally analysed by scanning electron microscopy (SEM) and compositionally by EDS, after the spectrometer was calibrated with a standard reference material (SRM) from NIST (National Institute of Standards and Technology). The results showed that the adjusting of acceleration voltage and of the powder particle size significantly influences the spectrum profile and the results of EDS analyses, which can lead to an erroneous primary identification of the analysed calcium phosphate type. View Full-Text
Keywords: calcium phosphates biomaterials; EDS results influencing parameters; calcium phosphorus ratio calcium phosphates biomaterials; EDS results influencing parameters; calcium phosphorus ratio
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MDPI and ACS Style

Miculescu, F.; Luță, C.; Constantinescu, A.E.; Maidaniuc, A.; Mocanu, A.-C.; Miculescu, M.; Voicu, Ș.I.; Ciocan, L.T. Considerations and Influencing Parameters in EDS Microanalysis of Biogenic Hydroxyapatite. J. Funct. Biomater. 2020, 11, 82. https://0-doi-org.brum.beds.ac.uk/10.3390/jfb11040082

AMA Style

Miculescu F, Luță C, Constantinescu AE, Maidaniuc A, Mocanu A-C, Miculescu M, Voicu ȘI, Ciocan LT. Considerations and Influencing Parameters in EDS Microanalysis of Biogenic Hydroxyapatite. Journal of Functional Biomaterials. 2020; 11(4):82. https://0-doi-org.brum.beds.ac.uk/10.3390/jfb11040082

Chicago/Turabian Style

Miculescu, Florin, Cristina Luță, Andreea E. Constantinescu, Andreea Maidaniuc, Aura-Cătălina Mocanu, Marian Miculescu, Ștefan I. Voicu, and Lucian T. Ciocan 2020. "Considerations and Influencing Parameters in EDS Microanalysis of Biogenic Hydroxyapatite" Journal of Functional Biomaterials 11, no. 4: 82. https://0-doi-org.brum.beds.ac.uk/10.3390/jfb11040082

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