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Article

Nanostructured Carbonated Hydroxyapatite Associated to rhBMP-2 Improves Bone Repair in Rat Calvaria

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Pos-Graduation Program, Dentistry School, Universidade Federal Fluminense, Rio de Janeiro 24020-140, Brazil
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Oral Surgery Department, Universidade Veiga de Almeida, Rio de Janeiro 20271-020, Brazil
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Oral Surgery Department, Universidade Iguaçu, Rio de Janeiro 26260-045, Brazil
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Oral Diagnosis Department, Dentistry School, Universidade Federal Fluminense, Rio de Janeiro 24020-140, Brazil
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Oral Surgery Department, Dentistry School, Universidade Federal Fluminense, Rio de Janeiro 24020-140, Brazil
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Orthodontic Department, Dentistry School, Universidade Federal Fluminense, Rio de Janeiro 24020-140, Brazil
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Brazilian Synchrotron Light Laboratory (LNLS), Brazilian Center for Research in Energy and Materials (CNPEM), São Paulo 13083-970, Brazil
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Department of Condensed Matter, Applied Physics and Nanoscience, Brazilian Center for Research in Physics, Rio de Janeiro 22290-180, Brazil
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Directory of Life Sciences Applied Metrology, Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO), Duque de Caxias 25250-020, Brazil
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Author to whom correspondence should be addressed.
J. Funct. Biomater. 2020, 11(4), 87; https://0-doi-org.brum.beds.ac.uk/10.3390/jfb11040087
Received: 26 October 2020 / Revised: 26 November 2020 / Accepted: 1 December 2020 / Published: 4 December 2020
Many biomaterials are used for Bone Morphogenetic Proteins (BMPs) delivery in bone tissue engineering. The BMP carrier system’s primary function is to hold these growth factors at the wound’s site for a prolonged time and provide initial support for cells to attach and elaborate the extracellular matrix for bone regeneration. This study aimed to evaluate the nanostructured carbonated hydroxyapatite microspheres (nCHA) as an rhBMP-2 carrier on rats calvaria. A total of fifteen male Wistar rats were randomly divided into three groups (n = 5): clot (control group), rhBMP-2 associated with collagen membrane (COL/rhBMP-2) or associated with the microspheres (nCHA/rhBMP-2). After 45 days, the calvaria defect samples were evaluated through histological, histomorphometric, and SR-µCT analyses to investigate new-formed bone and connective tissue volume densities. The descriptive histological analysis showed that nCHA/rhBMP-2 improved bone formation compared to other groups. These results were confirmed by histomorphometric and SR-µCT analysis that showed substantially defect area filling with a higher percentage of newly formed (36.24 ± 6.68) bone than those with the COL/rhBMP-2 (0.42 ± 0.40) and Clot (3.84 ± 4.57) (p < 0.05). The results showed that nCHA is an effective carrier for rhBMP-2 encouraging bone healing and an efficient alternative to collagen membrane for rhBMP-2 delivery. View Full-Text
Keywords: nanostructured carbonated hydroxyapatite; rhBMP-2; critical-size defect; bone regeneration nanostructured carbonated hydroxyapatite; rhBMP-2; critical-size defect; bone regeneration
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MDPI and ACS Style

Schneider Werner Vianna, T.; Sartoretto, S.C.; Neves Novellino Alves, A.T.; Figueiredo de Brito Resende, R.; de Almeida Barros Mourão, C.F.; de Albuquerque Calasans-Maia, J.; Martinez-Zelaya, V.R.; Malta Rossi, A.; Granjeiro, J.M.; Calasans-Maia, M.D.; Seabra Louro, R. Nanostructured Carbonated Hydroxyapatite Associated to rhBMP-2 Improves Bone Repair in Rat Calvaria. J. Funct. Biomater. 2020, 11, 87. https://0-doi-org.brum.beds.ac.uk/10.3390/jfb11040087

AMA Style

Schneider Werner Vianna T, Sartoretto SC, Neves Novellino Alves AT, Figueiredo de Brito Resende R, de Almeida Barros Mourão CF, de Albuquerque Calasans-Maia J, Martinez-Zelaya VR, Malta Rossi A, Granjeiro JM, Calasans-Maia MD, Seabra Louro R. Nanostructured Carbonated Hydroxyapatite Associated to rhBMP-2 Improves Bone Repair in Rat Calvaria. Journal of Functional Biomaterials. 2020; 11(4):87. https://0-doi-org.brum.beds.ac.uk/10.3390/jfb11040087

Chicago/Turabian Style

Schneider Werner Vianna, Thiago, Suelen C. Sartoretto, Adriana T. Neves Novellino Alves, Rodrigo Figueiredo de Brito Resende, Carlos F. de Almeida Barros Mourão, Jose de Albuquerque Calasans-Maia, Victor R. Martinez-Zelaya, Alexandre Malta Rossi, Jose M. Granjeiro, Monica D. Calasans-Maia, and Rafael Seabra Louro. 2020. "Nanostructured Carbonated Hydroxyapatite Associated to rhBMP-2 Improves Bone Repair in Rat Calvaria" Journal of Functional Biomaterials 11, no. 4: 87. https://0-doi-org.brum.beds.ac.uk/10.3390/jfb11040087

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