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Article

Survival and Proliferation under Severely Hypoxic Microenvironments Using Cell-Laden Oxygenating Hydrogels

1
Division of Engineering in Medicine, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Cambridge, MA 02139, USA
2
Materials Science and Engineering Graduate Program, UFPI-Federal University of Piaui, Teresina 64049-55, PI, Brazil
3
Academic Unit of Cabo de Santo Agostinho, Federal Rural University of Pernambuco, Cabo de Santo Agostinho 52171-900, PE, Brazil
4
Department of Physics, Federal University of Piaui, Teresina 64049-550, PI, Brazil
5
Biofabrication Lab, Department of Mechanical Engineering, Rowan University, Engineering Hall, Glassboro, NJ 08028, USA
6
Center for Applied NanoBioscience and Medicine, College of Medicine-Phoenix, University of Arizona, Phoenix, AZ 85004, USA
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Department of Pharmaceutical Technology, School of Pharmacy, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece
8
LIMAV-Interdisciplinary Laboratory for Advanced Materials, UFPI-Federal University of Piaui, Teresina 64049-550, PI, Brazil
*
Authors to whom correspondence should be addressed.
Contributed equally.
Academic Editors: Anişoara Cîmpean and R. Jayakumar
J. Funct. Biomater. 2021, 12(2), 30; https://0-doi-org.brum.beds.ac.uk/10.3390/jfb12020030
Received: 2 March 2021 / Revised: 18 April 2021 / Accepted: 27 April 2021 / Published: 2 May 2021
(This article belongs to the Special Issue Fibrous Scaffolds for Tissue Engineering Application)
Different strategies have been employed to provide adequate nutrients for engineered living tissues. These have mainly revolved around providing oxygen to alleviate the effects of chronic hypoxia or anoxia that result in necrosis or weak neovascularization, leading to failure of artificial tissue implants and hence poor clinical outcome. While different biomaterials have been used as oxygen generators for in vitro as well as in vivo applications, certain problems have hampered their wide application. Among these are the generation and the rate at which oxygen is produced together with the production of the reaction intermediates in the form of reactive oxygen species (ROS). Both these factors can be detrimental for cell survival and can severely affect the outcome of such studies. Here we present calcium peroxide (CPO) encapsulated in polycaprolactone as oxygen releasing microparticles (OMPs). While CPO releases oxygen upon hydrolysis, PCL encapsulation ensures that hydrolysis takes place slowly, thereby sustaining prolonged release of oxygen without the stress the bulk release can endow on the encapsulated cells. We used gelatin methacryloyl (GelMA) hydrogels containing these OMPs to stimulate survival and proliferation of encapsulated skeletal myoblasts and optimized the OMP concentration for sustained oxygen delivery over more than a week. The oxygen releasing and delivery platform described in this study opens up opportunities for cell-based therapeutic approaches to treat diseases resulting from ischemic conditions and enhance survival of implants under severe hypoxic conditions for successful clinical translation. View Full-Text
Keywords: oxygen; sever hypoxia; microparticles; electrospray; hydrogels; cells; biomaterials oxygen; sever hypoxia; microparticles; electrospray; hydrogels; cells; biomaterials
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MDPI and ACS Style

Hassan, S.; Cecen, B.; Peña-Garcia, R.; Marciano, F.R.; Miri, A.K.; Fattahi, A.; Karavasili, C.; Sebastian, S.; Zaidi, H.; Lobo, A.O. Survival and Proliferation under Severely Hypoxic Microenvironments Using Cell-Laden Oxygenating Hydrogels. J. Funct. Biomater. 2021, 12, 30. https://0-doi-org.brum.beds.ac.uk/10.3390/jfb12020030

AMA Style

Hassan S, Cecen B, Peña-Garcia R, Marciano FR, Miri AK, Fattahi A, Karavasili C, Sebastian S, Zaidi H, Lobo AO. Survival and Proliferation under Severely Hypoxic Microenvironments Using Cell-Laden Oxygenating Hydrogels. Journal of Functional Biomaterials. 2021; 12(2):30. https://0-doi-org.brum.beds.ac.uk/10.3390/jfb12020030

Chicago/Turabian Style

Hassan, Shabir, Berivan Cecen, Ramon Peña-Garcia, Fernanda R. Marciano, Amir K. Miri, Ali Fattahi, Christina Karavasili, Shikha Sebastian, Hamza Zaidi, and Anderson O. Lobo 2021. "Survival and Proliferation under Severely Hypoxic Microenvironments Using Cell-Laden Oxygenating Hydrogels" Journal of Functional Biomaterials 12, no. 2: 30. https://0-doi-org.brum.beds.ac.uk/10.3390/jfb12020030

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