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Communication

An Exonuclease I-Aided Turn-Off Fluorescent Strategy for Alkaline Phosphatase Assay Based on Terminal Protection and Copper Nanoparticles

School of Life Sciences, Central South University, Changsha 410013, China
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Authors to whom correspondence should be addressed.
Received: 9 April 2021 / Revised: 27 April 2021 / Accepted: 27 April 2021 / Published: 29 April 2021
(This article belongs to the Special Issue Advance Nanomaterials for Biosensors)
As an important DNA 3′-phosphatase, alkaline phosphatase can repair damaged DNA caused by replication and recombination. It is essential to measure the level of alkaline phosphatase to indicate some potential diseases, such as cancer, related to alkaline phosphatase. Here, we designed a simple and fast method to detect alkaline phosphatase quantitively. When alkaline phosphatase is present, the resulting poly T-DNA with a 3′-hydroxyl end was cleaved by exonuclease I, prohibiting the formation of fluorescent copper nanoparticles. However, the fluorescent copper nanoparticles can be monitored with the absence of alkaline phosphatase. Hence, we can detect alkaline phosphatase with this turn-off strategy. The proposed method is able to quantify the concentration of alkaline phosphatase with the LOD of 0.0098 U/L. Furthermore, we utilized this method to measure the effects of inhibitor Na3VO4 on alkaline phosphatase. In addition, it was successfully applied to quantify the level of alkaline phosphatase in human serum. The proposed strategy is sensitive, selective, cost effective, and timesaving, having a great potential to detect alkaline phosphatase quantitatively in clinical diagnosis. View Full-Text
Keywords: alkaline phosphatase; terminal protection; copper nanoparticle; fluorescence alkaline phosphatase; terminal protection; copper nanoparticle; fluorescence
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MDPI and ACS Style

Wang, Y.; Yan, Y.; Liu, X.; Ma, C. An Exonuclease I-Aided Turn-Off Fluorescent Strategy for Alkaline Phosphatase Assay Based on Terminal Protection and Copper Nanoparticles. Biosensors 2021, 11, 139. https://0-doi-org.brum.beds.ac.uk/10.3390/bios11050139

AMA Style

Wang Y, Yan Y, Liu X, Ma C. An Exonuclease I-Aided Turn-Off Fluorescent Strategy for Alkaline Phosphatase Assay Based on Terminal Protection and Copper Nanoparticles. Biosensors. 2021; 11(5):139. https://0-doi-org.brum.beds.ac.uk/10.3390/bios11050139

Chicago/Turabian Style

Wang, Yan, Ying Yan, Xinfa Liu, and Changbei Ma. 2021. "An Exonuclease I-Aided Turn-Off Fluorescent Strategy for Alkaline Phosphatase Assay Based on Terminal Protection and Copper Nanoparticles" Biosensors 11, no. 5: 139. https://0-doi-org.brum.beds.ac.uk/10.3390/bios11050139

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