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Perspective

Point-of-Care PCR Assays for COVID-19 Detection

1
Department of Biotechnology, Delhi Technological University, Shahbad Daulatpur, Delhi 110042, India
2
Nanotechnology Group, Tyndall National Institute, University College Cork, T12 K8AF Cork, Ireland
3
Department of Applied Chemistry, Delhi Technological University, Shahbad Daulatpur, New Delhi 110042, India
4
School of Chemistry, University College Cork, T12 K8AF Cork, Ireland
*
Authors to whom correspondence should be addressed.
Received: 16 March 2021 / Revised: 20 April 2021 / Accepted: 28 April 2021 / Published: 1 May 2021
(This article belongs to the Special Issue Biosensors for Diagnosis and Monitoring)
Molecular diagnostics has been the front runner in the world’s response to the COVID-19 pandemic. Particularly, reverse transcriptase-polymerase chain reaction (RT-PCR) and the quantitative variant (qRT-PCR) have been the gold standard for COVID-19 diagnosis. However, faster antigen tests and other point-of-care (POC) devices have also played a significant role in containing the spread of SARS-CoV-2 by facilitating mass screening and delivering results in less time. Thus, despite the higher sensitivity and specificity of the RT-PCR assays, the impact of POC tests cannot be ignored. As a consequence, there has been an increased interest in the development of miniaturized, high-throughput, and automated PCR systems, many of which can be used at point-of-care. This review summarizes the recent advances in the development of miniaturized PCR systems with an emphasis on COVID-19 detection. The distinct features of digital PCR and electrochemical PCR are detailed along with the challenges. The potential of CRISPR/Cas technology for POC diagnostics is also highlighted. Commercial RT–PCR POC systems approved by various agencies for COVID-19 detection are discussed. View Full-Text
Keywords: polymerase chain reaction; COVID-19; electrochemical; digital PCR; point-of-care polymerase chain reaction; COVID-19; electrochemical; digital PCR; point-of-care
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MDPI and ACS Style

Gupta, N.; Augustine, S.; Narayan, T.; O’Riordan, A.; Das, A.; Kumar, D.; Luong, J.H.T.; Malhotra, B.D. Point-of-Care PCR Assays for COVID-19 Detection. Biosensors 2021, 11, 141. https://0-doi-org.brum.beds.ac.uk/10.3390/bios11050141

AMA Style

Gupta N, Augustine S, Narayan T, O’Riordan A, Das A, Kumar D, Luong JHT, Malhotra BD. Point-of-Care PCR Assays for COVID-19 Detection. Biosensors. 2021; 11(5):141. https://0-doi-org.brum.beds.ac.uk/10.3390/bios11050141

Chicago/Turabian Style

Gupta, Niharika, Shine Augustine, Tarun Narayan, Alan O’Riordan, Asmita Das, D. Kumar, John H.T. Luong, and Bansi D. Malhotra 2021. "Point-of-Care PCR Assays for COVID-19 Detection" Biosensors 11, no. 5: 141. https://0-doi-org.brum.beds.ac.uk/10.3390/bios11050141

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