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Article

Cas12a and Lateral Flow Strip-Based Test for Rapid and Ultrasensitive Detection of Spinal Muscular Atrophy

Center for Medical Genetics & Hunan Key Laboratory of Medical Genetics, School of Life Sciences, Central South University, Changsha 410078, China
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Received: 8 April 2021 / Revised: 9 May 2021 / Accepted: 12 May 2021 / Published: 14 May 2021
(This article belongs to the Special Issue Application of CRISPR Cas Systems for Biosensing)
Spinal muscular atrophy (SMA) is characterized by severe lethality and irreversible progression. Early diagnosis of SMA is of more practical significance with the emergence of effective therapy. However, existing techniques to identify SMA patients rely on cumbersome instruments, hindering their accessibility and application. An SMA-Cas12a-strip assay was developed with the integration of Cas12a-based nucleic acid detection, isothermal amplification, and lateral flow strip. The analytical performance of the assay was assessed with clinical samples. To explore its extensible utility, various specimens were tested. Validated with 168 clinical samples, the sensitivity and specificity of the SMA-Cas12a-strip assay were both 100%. The minimum detectable concentration of genomic DNA containing the target gene achieved 526 aM. The assay was compatible with specimens from several sources, and the turnaround time could be within 1.5 h. We developed a simple, cost-effective, and highly sensitive and specific assay to detect SMA patients. With little and field-portable equipment, the assay holds great promise in the detection of SMA patients, particularly in low-resource regions. View Full-Text
Keywords: Cas12a; lateral flow strip; detection; spinal muscular atrophy Cas12a; lateral flow strip; detection; spinal muscular atrophy
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MDPI and ACS Style

Zhang, C.; Li, Z.; Chen, M.; Hu, Z.; Wu, L.; Zhou, M.; Liang, D. Cas12a and Lateral Flow Strip-Based Test for Rapid and Ultrasensitive Detection of Spinal Muscular Atrophy. Biosensors 2021, 11, 154. https://0-doi-org.brum.beds.ac.uk/10.3390/bios11050154

AMA Style

Zhang C, Li Z, Chen M, Hu Z, Wu L, Zhou M, Liang D. Cas12a and Lateral Flow Strip-Based Test for Rapid and Ultrasensitive Detection of Spinal Muscular Atrophy. Biosensors. 2021; 11(5):154. https://0-doi-org.brum.beds.ac.uk/10.3390/bios11050154

Chicago/Turabian Style

Zhang, Chunhua, Zhuo Li, Miaomiao Chen, Zhiqing Hu, Lingqian Wu, Miaojin Zhou, and Desheng Liang. 2021. "Cas12a and Lateral Flow Strip-Based Test for Rapid and Ultrasensitive Detection of Spinal Muscular Atrophy" Biosensors 11, no. 5: 154. https://0-doi-org.brum.beds.ac.uk/10.3390/bios11050154

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