Next Article in Journal
Label Free Glucose Electrochemical Biosensor Based on Poly(3,4-ethylenedioxy thiophene):Polystyrene Sulfonate/Titanium Carbide/Graphene Quantum Dots
Next Article in Special Issue
In-Situ Monitoring of Real-Time Loop-Mediated Isothermal Amplification with QCM: Detecting Listeria monocytogenes
Previous Article in Journal
A Novel Method That Allows SNP Discrimination with 160:1 Ratio for Biosensors Based on DNA-DNA Hybridization
Previous Article in Special Issue
Clinical Application of the Novel Cell-Based Biosensor for the Ultra-Rapid Detection of the SARS-CoV-2 S1 Spike Protein Antigen: A Practical Approach
Article

DNA Aptamer Functionalized Hydrogels for Interferometric Fiber-Optic Based Continuous Monitoring of Potassium Ions

Biophysics and Medical Technology, Department of Physics, NTNU The Norwegian University of Science and Technology, NO-7491 Trondheim, Norway
*
Author to whom correspondence should be addressed.
Received: 5 July 2021 / Revised: 31 July 2021 / Accepted: 3 August 2021 / Published: 6 August 2021
(This article belongs to the Special Issue Feature Issue of Biosensors and Bioelectronic Devices Section)
In the present paper, we describe a potassium sensor based on DNA-aptamer functionalized hydrogel, that is capable of continuous label-free potassium ion (K+) monitoring with potential for in situ application. A hydrogel attached to the end of an optical fiber is designed with di-oligonucleotides grafted to the polymer network that may serve as network junctions in addition to the covalent crosslinks. Specific affinity toward K+ is based on exploiting a particular aptamer that exhibits conformational transition from single-stranded DNA to G-quadruplex formed by the di-oligonucleotide in the presence of K+. Integration of this aptamer into the hydrogel transforms the K+ specific conformational transition to a K+ concentration dependent deswelling of the hydrogel. High-resolution interferometry monitors changes in extent of swelling at 1 Hz and 2 nm resolution for the hydrogel matrix of 50 µm. The developed hydrogel-based biosensor displayed high selectivity for K+ ions in the concentration range up to 10 mM, in the presence of physiological concentrations of Na+. Additionally, the concentration dependent and selective K+ detection demonstrated in the artificial blood buffer environment, both at room and physiological temperatures, suggests substantial potential for practical applications such as monitoring of potassium ion concentration in blood levels in intensive care medicine. View Full-Text
Keywords: potassium biosensor; aptamer; responsive hydrogel; interferometry potassium biosensor; aptamer; responsive hydrogel; interferometry
Show Figures

Figure 1

MDPI and ACS Style

Žuržul, N.; Stokke, B.T. DNA Aptamer Functionalized Hydrogels for Interferometric Fiber-Optic Based Continuous Monitoring of Potassium Ions. Biosensors 2021, 11, 266. https://0-doi-org.brum.beds.ac.uk/10.3390/bios11080266

AMA Style

Žuržul N, Stokke BT. DNA Aptamer Functionalized Hydrogels for Interferometric Fiber-Optic Based Continuous Monitoring of Potassium Ions. Biosensors. 2021; 11(8):266. https://0-doi-org.brum.beds.ac.uk/10.3390/bios11080266

Chicago/Turabian Style

Žuržul, Nataša, and Bjørn T. Stokke 2021. "DNA Aptamer Functionalized Hydrogels for Interferometric Fiber-Optic Based Continuous Monitoring of Potassium Ions" Biosensors 11, no. 8: 266. https://0-doi-org.brum.beds.ac.uk/10.3390/bios11080266

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop