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Article

An Electrochemical Enzyme Biosensor for Ammonium Detection in Aquaculture Using Screen-Printed Electrode Modified by Gold Nanoparticle/Polymethylene Blue

National Innovation Center for Digital Fishery, China Agricultural University, Beijing 100083, China
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Authors to whom correspondence should be addressed.
Received: 11 August 2021 / Revised: 1 September 2021 / Accepted: 10 September 2021 / Published: 13 September 2021
(This article belongs to the Special Issue Biosensors for Agriculture, Environment and Food)
A SPEC/AuNPs/PMB modified electrode was prepared by electrodeposition and electro-polymerization. The electrochemical behavior of reduced nicotinamide adenine dinucleotide (NADH) on the surface of the modified electrode was studied by cyclic voltammetry. A certain amount of substrate and glutamate dehydrogenase (GLDH) were coated on the modified electrode to form a functional enzyme membrane. The ammonia nitrogen in the water sample could be calculated indirectly by measuring the consumption of NADH in the reaction. The results showed that the strength of electro-catalytic current signal was increased by two times; the catalytic oxidation potential was shifted to the left by 0.5 V, and the anti-interference ability of the sensor was enhanced. The optimum substrate concentration and enzyme loading were determined as 1.3 mM NADH, 28 mM α-Ketoglutarate and 2.0 U GLDH, respectively. The homemade ceramic heating plate controlled the working electrode to work at 37 °C. A pH compensation algorithm based on piecewise linear interpolation could reduce the measurement error to less than 3.29 μM. The biosensor exhibited good linearity in the range of 0~300 μM with a detection limit of 0.65 μM NH4+. Compared with standard Nessler’s method, the recoveries were 93.71~105.92%. The biosensor was found to be stable for at least 14 days when refrigerated and sealed at 4 °C. View Full-Text
Keywords: biosensor; glutamate dehydrogenase; methylene blue; screen-printed electrode; ammonium biosensor; glutamate dehydrogenase; methylene blue; screen-printed electrode; ammonium
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MDPI and ACS Style

Wang, C.; Wang, T.; Li, Z.; Xu, X.; Zhang, X.; Li, D. An Electrochemical Enzyme Biosensor for Ammonium Detection in Aquaculture Using Screen-Printed Electrode Modified by Gold Nanoparticle/Polymethylene Blue. Biosensors 2021, 11, 335. https://0-doi-org.brum.beds.ac.uk/10.3390/bios11090335

AMA Style

Wang C, Wang T, Li Z, Xu X, Zhang X, Li D. An Electrochemical Enzyme Biosensor for Ammonium Detection in Aquaculture Using Screen-Printed Electrode Modified by Gold Nanoparticle/Polymethylene Blue. Biosensors. 2021; 11(9):335. https://0-doi-org.brum.beds.ac.uk/10.3390/bios11090335

Chicago/Turabian Style

Wang, Cong, Tan Wang, Zhen Li, Xianbao Xu, Xiaoshuan Zhang, and Daoliang Li. 2021. "An Electrochemical Enzyme Biosensor for Ammonium Detection in Aquaculture Using Screen-Printed Electrode Modified by Gold Nanoparticle/Polymethylene Blue" Biosensors 11, no. 9: 335. https://0-doi-org.brum.beds.ac.uk/10.3390/bios11090335

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