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Article

Carbapenem-Resistant Klebsiella pneumoniae Clinical Isolates: In Vivo Virulence Assessment in Galleria mellonella and Potential Therapeutics by Polycationic Oligoethyleneimine

1
iBB—Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal
2
J. Heyrovský Institute of Physical Chemistry of the Czech Academy of Sciences, Dolejskova 3, 18223 Prague, Czech Republic
3
Secção de Microbiologia, Laboratório SYNLAB-Lisboa, Grupo SYNLAB Portugal, Av. Columbano Bordalo Pinheiro, 75 A, 2° Andar, 1070-061 Lisboa, Portugal
*
Authors to whom correspondence should be addressed.
Received: 30 September 2020 / Revised: 24 December 2020 / Accepted: 6 January 2021 / Published: 8 January 2021
(This article belongs to the Special Issue New Insights on Biofilm Antimicrobial Strategies)
Klebsiella pneumoniae, one of the most common pathogens found in hospital-acquired infections, is often resistant to multiple antibiotics. In fact, multidrug-resistant (MDR) K. pneumoniae producing KPC or OXA-48-like carbapenemases are recognized as a serious global health threat. In this sense, we evaluated the virulence of K. pneumoniae KPC(+) or OXA-48(+) aiming at potential antimicrobial therapeutics. K. pneumoniae carbapenemase (KPC) and the expanded-spectrum oxacillinase OXA-48 isolates were obtained from patients treated in medical care units in Lisbon, Portugal. The virulence potential of the K. pneumonia clinical isolates was tested using the Galleria mellonella model. For that, G. mellonella larvae were inoculated using patients KPC(+) and OXA-48(+) isolates. Using this in vivo model, the KPC(+) K. pneumoniae isolates showed to be, on average, more virulent than OXA-48(+). Virulence was found attenuated when a low bacterial inoculum (one magnitude lower) was tested. In addition, we also report the use of a synthetic polycationic oligomer (L-OEI-h) as a potential antimicrobial agent to fight infectious diseases caused by MDR bacteria. L-OEI-h has a broad-spectrum antibacterial activity and exerts a significantly bactericidal activity within the first 5-30 min treatment, causing lysis of the cytoplasmic membrane. Importantly, the polycationic oligomer showed low toxicity against in vitro models and no visible cytotoxicity (measured by survival and health index) was noted on the in vivo model (G. mellonella), thus L-OEI-h is foreseen as a promising polymer therapeutic for the treatment of MDR K. pneumoniae infections. View Full-Text
Keywords: Klebsiella pneumoniae; KPC and OXA-48-like carbapenemases; Galleria mellonella infection model; linear oligoethyleneimine hydrochloride Klebsiella pneumoniae; KPC and OXA-48-like carbapenemases; Galleria mellonella infection model; linear oligoethyleneimine hydrochloride
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MDPI and ACS Style

Mil-Homens, D.; Martins, M.; Barbosa, J.; Serafim, G.; Sarmento, M.J.; Pires, R.F.; Rodrigues, V.; Bonifácio, V.D.B.; Pinto, S.N. Carbapenem-Resistant Klebsiella pneumoniae Clinical Isolates: In Vivo Virulence Assessment in Galleria mellonella and Potential Therapeutics by Polycationic Oligoethyleneimine. Antibiotics 2021, 10, 56. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10010056

AMA Style

Mil-Homens D, Martins M, Barbosa J, Serafim G, Sarmento MJ, Pires RF, Rodrigues V, Bonifácio VDB, Pinto SN. Carbapenem-Resistant Klebsiella pneumoniae Clinical Isolates: In Vivo Virulence Assessment in Galleria mellonella and Potential Therapeutics by Polycationic Oligoethyleneimine. Antibiotics. 2021; 10(1):56. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10010056

Chicago/Turabian Style

Mil-Homens, Dalila, Maria Martins, José Barbosa, Gabriel Serafim, Maria J. Sarmento, Rita F. Pires, Vitória Rodrigues, Vasco D.B. Bonifácio, and Sandra N. Pinto 2021. "Carbapenem-Resistant Klebsiella pneumoniae Clinical Isolates: In Vivo Virulence Assessment in Galleria mellonella and Potential Therapeutics by Polycationic Oligoethyleneimine" Antibiotics 10, no. 1: 56. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10010056

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