Next Article in Journal
Genome-Wide Identification of Resveratrol Intrinsic Resistance Determinants in Staphylococcus aureus
Previous Article in Journal
Synergistic Effect of Abietic Acid with Oxacillin against Methicillin-Resistant Staphylococcus pseudintermedius
Previous Article in Special Issue
Antimicrobial and Antivirulence Impacts of Phenolics on Salmonella Enterica Serovar Typhimurium
Article

Back to Nature: Combating Candida albicans Biofilm, Phospholipase and Hemolysin Using Plant Essential Oils

1
Microbiology and Biotechnology Department, Faculty of Pharmacy, Delta University for Science and Technology, International Coastal Road, Gamasa City, Mansoura, 11152 Dakhaliya, Egypt
2
Infection Control Unit, Zagazig University Hospitals, 44519 Zagazig, Egypt
3
Pharmaceutical Chemistry Department, Faculty of Pharmacy, Delta University for Science and Technology, International Coastal Road, Gamasa City, Mansoura, 11152 Dakhaliya, Egypt
4
Department of Chemistry and Biochemistry, University of Regina, Regina, SK S4S 1P4, Canada
5
Microbiology and Immunology Department, Faculty of Pharmacy, Zagazig University, 44519 Zagazig, Egypt
*
Author to whom correspondence should be addressed.
Received: 24 December 2020 / Revised: 13 January 2021 / Accepted: 13 January 2021 / Published: 15 January 2021
(This article belongs to the Special Issue Antibacterial and Antibiofilm Activity of Plant Extracts)
Candida albicans is the causative agent of fatal systemic candidiasis. Due to limitations of antifungals, new drugs are needed. The anti-virulence effect of plant essential oils (EOs) was evaluated against clinical C. albicans isolates including cinnamon, clove, jasmine and rosemary oils. Biofilm, phospholipase and hemolysin were assessed phenotypically. EOs were evaluated for their anti-virulence activity using phenotypic methods as well as scanning electron microscopy (SEM) and atomic force microscopy (AFM). Among the C. albicans isolates, biofilm, phospholipase and hemolysins were detected in 40.4, 86.5 and 78.8% of isolates, respectively. Jasmine oil showed the highest anti-biofilm activity followed by cinnamon, clove and rosemary oils. SEM and AFM analysis showed reduced adherence and roughness in the presence of EOs. For phospholipase, rosemary oil was the most inhibitory, followed by jasmine, cinnamon and clove oils, and for hemolysins, cinnamon had the highest inhibition followed by jasmine, rosemary and clove oils. A molecular docking study revealed major EO constituents as promising inhibitors of the Als3 adhesive protein, with the highest binding for eugenol, followed by 1,8-cineole, 2-phenylthiolane and cinnamaldehyde. In conclusion, EOs have a promising inhibitory impact on Candida biofilm, phospholipase and hemolysin production, hence EOs could be used as potential antifungals that impact virulence factors. View Full-Text
Keywords: Candida albicans; essential oils; jasmine oil; biofilm; SEM; AFM; phospholipase; hemolysin; molecular docking Candida albicans; essential oils; jasmine oil; biofilm; SEM; AFM; phospholipase; hemolysin; molecular docking
Show Figures

Figure 1

MDPI and ACS Style

El-Baz, A.M.; Mosbah, R.A.; Goda, R.M.; Mansour, B.; Sultana, T.; Dahms, T.E.S.; El-Ganiny, A.M. Back to Nature: Combating Candida albicans Biofilm, Phospholipase and Hemolysin Using Plant Essential Oils. Antibiotics 2021, 10, 81. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10010081

AMA Style

El-Baz AM, Mosbah RA, Goda RM, Mansour B, Sultana T, Dahms TES, El-Ganiny AM. Back to Nature: Combating Candida albicans Biofilm, Phospholipase and Hemolysin Using Plant Essential Oils. Antibiotics. 2021; 10(1):81. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10010081

Chicago/Turabian Style

El-Baz, Ahmed M., Rasha A. Mosbah, Reham M. Goda, Basem Mansour, Taranum Sultana, Tanya E.S. Dahms, and Amira M. El-Ganiny 2021. "Back to Nature: Combating Candida albicans Biofilm, Phospholipase and Hemolysin Using Plant Essential Oils" Antibiotics 10, no. 1: 81. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10010081

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop