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Open AccessArticle

Spread of Linezolid-Resistant Enterococcus spp. in Human Clinical Isolates in the Czech Republic

1
National Reference Laboratory for Antibiotics, Centre for Epidemiology and Microbiology, National Institute of Public Health, 10000 Prague, Czech Republic
2
Department of Microbiology, 3rd Faculty of Medicine Charles University, University Hospital Kralovske Vinohrady and National Institute of Public Health, 10000 Prague, Czech Republic
3
Department of Clinical Microbiology, Faculty of Medicine and University Hospital, Charles University, 53002 Hradec Kralove, Czech Republic
4
National Reference Laboratory for Meningococcal Infections, Centre for Epidemiology and Microbiology, National Institute of Public Health, 10000 Prague, Czech Republic
*
Author to whom correspondence should be addressed.
Academic Editor: Jeffrey Lipman
Received: 27 January 2021 / Revised: 11 February 2021 / Accepted: 20 February 2021 / Published: 22 February 2021
The aim of this study was to map and investigate linezolid resistance mechanisms in linezolid-resistant enterococci in the Czech Republic from 2009 to 2019. Altogether, 1442 isolates of Enterococcus faecium and Enterococcus faecalis were examined in the National Reference Laboratory for Antibiotics. Among them, 8% of isolates (n = 115) were resistant to linezolid (E. faecium/n = 106, E. faecalis/n = 9). Only three strains of E. faecium were resistant to tigecycline, 72.6% of isolates were resistant to vancomycin. One isolate of E. faecium harbored the cfr gene. The majority (87%, n = 11) of E. faecium strains were resistant to linezolid because of the mutation G2576T in the domain V of the 23S rRNA. This mutation was detected also in two strains of E. faecalis. The presence of the optrA gene was the dominant mechanism of linezolid resistance in E. faecalis isolates. None of enterococci contained cfrB, poxtA genes, or any amino acid mutation in genes encoding ribosomal proteins. No mechanism of resistance was identified in 4 out of 106 E. faecium linezolid resistant isolates in this study. Seventeen sequence types (STs) including four novel STs were identified in this work. Clonal complex CC17 was found in all E. faecium isolates. View Full-Text
Keywords: Enterococcus faecium; Enterococcus faecalis; linezolid resistance; 23S rRNA; optrA Enterococcus faecium; Enterococcus faecalis; linezolid resistance; 23S rRNA; optrA
MDPI and ACS Style

Mališová, L.; Jakubů, V.; Pomorská, K.; Musílek, M.; Žemličková, H. Spread of Linezolid-Resistant Enterococcus spp. in Human Clinical Isolates in the Czech Republic. Antibiotics 2021, 10, 219. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10020219

AMA Style

Mališová L, Jakubů V, Pomorská K, Musílek M, Žemličková H. Spread of Linezolid-Resistant Enterococcus spp. in Human Clinical Isolates in the Czech Republic. Antibiotics. 2021; 10(2):219. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10020219

Chicago/Turabian Style

Mališová, Lucia; Jakubů, Vladislav; Pomorská, Katarína; Musílek, Martin; Žemličková, Helena. 2021. "Spread of Linezolid-Resistant Enterococcus spp. in Human Clinical Isolates in the Czech Republic" Antibiotics 10, no. 2: 219. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10020219

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