Next Article in Journal
Impact on Antibiotic Resistance, Therapeutic Success, and Control of Side Effects in Therapeutic Drug Monitoring (TDM) of Daptomycin: A Scoping Review
Next Article in Special Issue
Taxonomic Identification of Different Species of the Genus Aeromonas by Whole-Genome Sequencing and Use of Their Species-Specific β-Lactamases as Phylogenetic Markers
Previous Article in Journal
Amycolatomycins A and B, Cyclic Hexapeptides Isolated from an Amycolatopsis sp. 195334CR
Previous Article in Special Issue
Genome-Based Analyses of Fitness Effects and Compensatory Changes Associated with Acquisition of blaCMY-, blaCTX-M-, and blaOXA-48/VIM-1-Containing Plasmids in Escherichia coli
Article

Antimicrobial Resistance Genes and Diversity of Clones among ESBL- and Acquired AmpC-Producing Escherichia coli Isolated from Fecal Samples of Healthy and Sick Cats in Portugal

1
Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal
2
Department of Genetics and Biotechnology, UTAD, 5000-801 Vila Real, Portugal
3
Functional Genomics and Proteomics Unit, UTAD, 5000-801 Vila Real, Portugal
4
Laboratory Associated for Green Chemistry (LAQV-REQUIMTE), New University of Lisbon, 2829-516 Monte da Caparica, Portugal
5
Area Biochemistry and Molecular Biology, University of La Rioja, 26006 Logroño, Spain
6
Laboratory of Exploration and Valuation of the Steppe Ecosystem, University of Djelfa, Djelfa 17000, Algeria
7
Hospital Veterinário de São Bento, 1200-822 Lisboa, Portugal
8
Clínica Veterinária do Vouga, 3740-253 Sever do Vouga, Portugal
9
Hospital Veterinário de Trás-os-Montes, 5000-056 Vila Real, Portugal
10
Centro Veterinário de Macedo de Cavaleiros, 5340 Bragança, Portugal
11
VetRedondo, Consultório Veterinário de Monte Redondo Unipessoal Lda, Monte Redondo, 2425-618 Leiria, Portugal
*
Author to whom correspondence should be addressed.
Academic Editor: Ângela Novais
Received: 2 January 2021 / Revised: 28 February 2021 / Accepted: 2 March 2021 / Published: 5 March 2021
The aim of the study was to analyze the mechanisms of resistance in extended-spectrum beta-lactamase (ESBL)- and acquired AmpC (qAmpC)-producing Escherichia coli isolates from healthy and sick cats in Portugal. A total of 141 rectal swabs recovered from 98 sick and 43 healthy cats were processed for cefotaxime-resistant (CTXR) E. coli recovery (in MacConkey agar supplemented with 2 µg/mL cefotaxime). The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) method was used for E. coli identification and antimicrobial susceptibility was performed by a disk diffusion test. The presence of resistance/virulence genes was tested by PCR sequencing. The phylogenetic typing and multilocus sequence typing (MLST) were determined by specific PCR sequencing. CTXRE. coli isolates were detected in seven sick and six healthy cats (7.1% and 13.9%, respectively). Based on the synergy tests, 11 of 13 CTXRE. coli isolates (one/sample) were ESBL-producers (ESBL total rate: 7.8%) carrying the following ESBL genes: blaCTX-M-1 (n = 3), blaCTX-M-15 (n = 3), blaCTX-M-55 (n = 2), blaCTX-M-27 (n = 2) and blaCTX-M-9 (n = 1). Six different sequence types were identified among ESBL-producers (sequence type/associated ESBLs): ST847/CTX-M-9, CTX-M-27, CTX-M-1; ST10/CTX-M-15, CTX-M-27; ST6448/CTX-M-15, CTX-M-55; ST429/CTX-M-15; ST101/CTX-M-1 and ST40/CTX-M-1. Three of the CTXR isolates were CMY-2-producers (qAmpC rate: 2.1%); two of them were ESBL-positive and one ESBL-negative. These isolates were typed as ST429 and ST6448 and were obtained in healthy or sick cats. The phylogenetic groups A/B1/D/clade 1 were detected among ESBL- and qAmpC-producing isolates. Cats are carriers of qAmpC (CMY-2)- and ESBL-producing E. coli isolates (mostly of variants of CTX-M group 1) of diverse clonal lineages, which might represent a public health problem due to the proximity of cats with humans regarding a One Health perspective. View Full-Text
Keywords: antimicrobial resistance; E. coli; cats; public health; extended spectrum beta-lactamase (ESBL), AmpC beta-lactamase; CTX-M; CMY-2 antimicrobial resistance; E. coli; cats; public health; extended spectrum beta-lactamase (ESBL), AmpC beta-lactamase; CTX-M; CMY-2
Show Figures

Figure 1

MDPI and ACS Style

Carvalho, I.; Safia Chenouf, N.; Cunha, R.; Martins, C.; Pimenta, P.; Pereira, A.R.; Martínez-Álvarez, S.; Ramos, S.; Silva, V.; Igrejas, G.; Torres, C.; Poeta, P. Antimicrobial Resistance Genes and Diversity of Clones among ESBL- and Acquired AmpC-Producing Escherichia coli Isolated from Fecal Samples of Healthy and Sick Cats in Portugal. Antibiotics 2021, 10, 262. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10030262

AMA Style

Carvalho I, Safia Chenouf N, Cunha R, Martins C, Pimenta P, Pereira AR, Martínez-Álvarez S, Ramos S, Silva V, Igrejas G, Torres C, Poeta P. Antimicrobial Resistance Genes and Diversity of Clones among ESBL- and Acquired AmpC-Producing Escherichia coli Isolated from Fecal Samples of Healthy and Sick Cats in Portugal. Antibiotics. 2021; 10(3):262. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10030262

Chicago/Turabian Style

Carvalho, Isabel, Nadia Safia Chenouf, Rita Cunha, Carla Martins, Paulo Pimenta, Ana R. Pereira, Sandra Martínez-Álvarez, Sónia Ramos, Vanessa Silva, Gilberto Igrejas, Carmen Torres, and Patrícia Poeta. 2021. "Antimicrobial Resistance Genes and Diversity of Clones among ESBL- and Acquired AmpC-Producing Escherichia coli Isolated from Fecal Samples of Healthy and Sick Cats in Portugal" Antibiotics 10, no. 3: 262. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10030262

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop