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Article

Phage PPPL-1, A New Biological Agent to Control Bacterial Canker Caused by Pseudomonas syringae pv. actinidiae in Kiwifruit

1
Department of Horticultural Biotechnology, College of Life Science, Kyung Hee University, Yongin 17104, Korea
2
Fruit Research Institute, Jeollanamdo Agricultural Research and Extension Services, Haenam-gun 59021, Korea
3
Graduate School of Biotechnology, Kyung Hee University, Yongin 17104, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Carla Pereira
Received: 13 April 2021 / Revised: 6 May 2021 / Accepted: 7 May 2021 / Published: 10 May 2021
(This article belongs to the Special Issue Phage Therapy to Control Pathogenic Bacteria)
Pseudomonas syringae pv. actinidiae (Psa) is a Gram-negative bacterium that causes bacterial canker disease in kiwifruit. Copper or antibiotics have been used in orchards to control this disease, but the recent emergence of antibiotic-resistant Psa has called for the development of a new control agent. We previously reported that the bacteriophage (or phage) PPPL-1 showed antibacterial activity for both biovar 2 and 3 of Psa. To investigate the possibility of PPPL-1 to control bacterial canker in kiwifruit, we further tested the efficacy of PPPL-1 and its phage cocktail with two other phages on suppressing disease development under greenhouse conditions using 6 weeks old kiwifruit plants. Our results showed that the disease control efficacy of PPPL-1 treatment was statistically similar to those of phage cocktail treatment or AgrimycinTM, which contains streptomycin and oxytetracycline antibiotics as active ingredients. Moreover, PPPL-1 could successfully kill streptomycin-resistant Psa isolates, of which the treatment of BuramycinTM carrying only streptomycin as an active ingredient had no effect in vitro. The phage PPPL-1 was further characterized, and stability assays showed that the phage was stable in the field soil and at low temperature of 0 ± 2 °C. In addition, the phage could be scaled up quickly up to 1010 pfu/mL at 12 h later from initial multiplicity of infection of 0.000005. Our results indicate that PPPL-1 phage is a useful candidate as a biocontrol agent and could be a tool to control the bacterial canker in kiwifruit by Psa infection in the field conditions. View Full-Text
Keywords: bacterial canker; disease control; kiwifruit; phage; Pseudomonas syringae pv. actinidiae bacterial canker; disease control; kiwifruit; phage; Pseudomonas syringae pv. actinidiae
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MDPI and ACS Style

Song, Y.-R.; Vu, N.T.; Park, J.; Hwang, I.S.; Jeong, H.-J.; Cho, Y.-S.; Oh, C.-S. Phage PPPL-1, A New Biological Agent to Control Bacterial Canker Caused by Pseudomonas syringae pv. actinidiae in Kiwifruit. Antibiotics 2021, 10, 554. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10050554

AMA Style

Song Y-R, Vu NT, Park J, Hwang IS, Jeong H-J, Cho Y-S, Oh C-S. Phage PPPL-1, A New Biological Agent to Control Bacterial Canker Caused by Pseudomonas syringae pv. actinidiae in Kiwifruit. Antibiotics. 2021; 10(5):554. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10050554

Chicago/Turabian Style

Song, Yu-Rim, Nguyen T. Vu, Jungkum Park, In S. Hwang, Hyeon-Ju Jeong, Youn-Sup Cho, and Chang-Sik Oh. 2021. "Phage PPPL-1, A New Biological Agent to Control Bacterial Canker Caused by Pseudomonas syringae pv. actinidiae in Kiwifruit" Antibiotics 10, no. 5: 554. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10050554

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