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Article

Detection of Antimicrobial Resistance of Bacteria Staphylococcus chromogenes Isolated from Sheep’s Milk and Cheese

1
Department of Food Hygiene, Technology and Safety, The University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia
2
Department of Animal Nutrition and Husbandry, The University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia
3
Department of Public Veterinary Medicine and Animal Welfare, The University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia
*
Author to whom correspondence should be addressed.
Academic Editors: John Barlow and Laura Selva Martínez
Received: 30 March 2021 / Revised: 6 May 2021 / Accepted: 8 May 2021 / Published: 12 May 2021
Antimicrobial and multidrug resistance is detected in nonaureus staphylococci, including Staphylococcus chromogenes, which commonly causes intramammary infections. Recent clinical studies point to the presence of methicillin-resistant S. chromogenes. Therefore, this study aims to determine the prevalence of this species in samples of sheep‘s milk and cheeses made from them. Isolates were identified by polymerase chain reaction and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI–TOF). A total of 208 staphylococcal isolates were identified. Of these, 18% were identified as S. chromogenes. The antimicrobial resistance of the identified isolates was determined using the agar dilution method against penicillin, ceftaroline, teicoplanin, gentamicin, erythromycin, tetracycline, and ofloxacin. The highest resistance was found to penicillin (95%), tetracycline (86%), and oxacillin (81%). The highest sensitivity was confirmed for gentamicin (55%). The study also confirmed the presence of methicillin resistant staphylococcal isolates (30%) based on the phenotypic manifestation of antimicrobial resistance and detection of the presence of the mecA gene. The study shows that the tested isolates (62%) were multidrug resistant. Resistance to two antibiotics was most often found (39%). View Full-Text
Keywords: antibiotic; mecA gene; MALDI–TOF; PCR; staphylococci antibiotic; mecA gene; MALDI–TOF; PCR; staphylococci
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MDPI and ACS Style

Regecová, I.; Výrostková, J.; Zigo, F.; Gregová, G.; Kováčová, M. Detection of Antimicrobial Resistance of Bacteria Staphylococcus chromogenes Isolated from Sheep’s Milk and Cheese. Antibiotics 2021, 10, 570. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10050570

AMA Style

Regecová I, Výrostková J, Zigo F, Gregová G, Kováčová M. Detection of Antimicrobial Resistance of Bacteria Staphylococcus chromogenes Isolated from Sheep’s Milk and Cheese. Antibiotics. 2021; 10(5):570. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10050570

Chicago/Turabian Style

Regecová, Ivana, Jana Výrostková, František Zigo, Gabriela Gregová, and Mariana Kováčová. 2021. "Detection of Antimicrobial Resistance of Bacteria Staphylococcus chromogenes Isolated from Sheep’s Milk and Cheese" Antibiotics 10, no. 5: 570. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10050570

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