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The Application of Ribosome Engineering to Natural Product Discovery and Yield Improvement in Streptomyces

by 1, 1,2,3,* and 1,3,*
1
Xiangya International Academy of Translational Medicine at Central South University, Changsha 410013, Hunan, China
2
Hunan Engineering Research Center of Combinatorial Biosynthesis and Natural Product Drug Discovery, Changsha 410011, Hunan, China
3
National Engineering Research Center of Combinatorial Biosynthesis for Drug Discovery, Changsha 410011, Hunan, China
*
Authors to whom correspondence should be addressed.
Received: 29 July 2019 / Revised: 10 August 2019 / Accepted: 27 August 2019 / Published: 30 August 2019
(This article belongs to the Special Issue Mechanism and Regulation of Antibiotic Synthesis in Streptomyces)
Microbial natural product drug discovery and development has entered a new era, driven by microbial genomics and synthetic biology. Genome sequencing has revealed the vast potential to produce valuable secondary metabolites in bacteria and fungi. However, many of the biosynthetic gene clusters are silent under standard fermentation conditions. By rational screening for mutations in bacterial ribosomal proteins or RNA polymerases, ribosome engineering is a versatile approach to obtain mutants with improved titers for microbial product formation or new natural products through activating silent biosynthetic gene clusters. In this review, we discuss the mechanism of ribosome engineering and its application to natural product discovery and yield improvement in Streptomyces. Our analysis suggests that ribosome engineering is a rapid and cost-effective approach and could be adapted to speed up the discovery and development of natural product drug leads in the post-genomic era. View Full-Text
Keywords: ribosome engineering; strain improvement; natural products; Streptomyces ribosome engineering; strain improvement; natural products; Streptomyces
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MDPI and ACS Style

Zhu, S.; Duan, Y.; Huang, Y. The Application of Ribosome Engineering to Natural Product Discovery and Yield Improvement in Streptomyces. Antibiotics 2019, 8, 133. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics8030133

AMA Style

Zhu S, Duan Y, Huang Y. The Application of Ribosome Engineering to Natural Product Discovery and Yield Improvement in Streptomyces. Antibiotics. 2019; 8(3):133. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics8030133

Chicago/Turabian Style

Zhu, Saibin, Yanwen Duan, and Yong Huang. 2019. "The Application of Ribosome Engineering to Natural Product Discovery and Yield Improvement in Streptomyces" Antibiotics 8, no. 3: 133. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics8030133

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