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Article

Characterization of a Carbapenem-Resistant Kluyvera Cryocrescens Isolate Carrying Blandm-1 from Hospital Sewage

1
Department of Immunology, School of Basic Medical Sciences, Southwest Medical University, Luzhou 646000, Sichuan, China
2
Department of Pathogenic Biology, School of Basic Medical Sciences, Southwest Medical University, Luzhou 646000, Sichuan, China
*
Author to whom correspondence should be addressed.
Both authors contributed equally to this work.
Received: 24 August 2019 / Revised: 9 September 2019 / Accepted: 11 September 2019 / Published: 16 September 2019
Carbapenem-resistant Enterobacteriaceae have been a global public health issue in recent years. Here, a carbapenem-resistant Kluyvera cryocrescens strain SCW13 was isolated from hospital sewage, and was then subjected to whole-genome sequencing (WGS). Based on WGS data, antimicrobial resistance genes were identified. Resistance plasmids were completely circularized and further bioinformatics analyses of plasmids were performed. A conjugation assay was performed to identify a self-transmissible plasmid mediating carbapenem resistance. A phylogenetic tree was constructed based on the core genome of publicly available Kluyvera strains. The isolate SCW13 exhibited resistance to cephalosporin and carbapenem. blaNDM-1 was found to be located on a ~53-kb self-transmissible IncX3 plasmid, which exhibited high similarity to the previously reported pNDM-HN380, which is an epidemic blaNDM-1-carrying IncX3 plasmid. Further, we found that SCW13 contained a chromosomal blaKLUC-2 gene, which was the probable origin of the plasmid-born blaKLUC-2 found in Enterobacter cloacae. Phylogenetic analysis showed that K. cryocrescens SCW13 exhibited a close relationship with K. cryocrescens NCTC10483. These findings highlight the further dissemination of blaNDM through clonal IncX3 plasmids related to pNDM-HN380 among uncommon Enterobacteriaceae strains, including Kluyvera in this case. View Full-Text
Keywords: Kluyvera; NDM-1; IncX3; blaKLUC-2; carbapenemase Kluyvera; NDM-1; IncX3; blaKLUC-2; carbapenemase
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MDPI and ACS Style

Li, Y.; Luo, L.; Xiao, Z.; Wang, G.; Li, C.; Zhang, Z.; Zhou, Y.; Zhang, L. Characterization of a Carbapenem-Resistant Kluyvera Cryocrescens Isolate Carrying Blandm-1 from Hospital Sewage. Antibiotics 2019, 8, 149. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics8030149

AMA Style

Li Y, Luo L, Xiao Z, Wang G, Li C, Zhang Z, Zhou Y, Zhang L. Characterization of a Carbapenem-Resistant Kluyvera Cryocrescens Isolate Carrying Blandm-1 from Hospital Sewage. Antibiotics. 2019; 8(3):149. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics8030149

Chicago/Turabian Style

Li, Ying; Luo, Li; Xiao, Zhijiao; Wang, Guangxi; Li, Chengwen; Zhang, Zhikun; Zhou, Yingshun; Zhang, Luhua. 2019. "Characterization of a Carbapenem-Resistant Kluyvera Cryocrescens Isolate Carrying Blandm-1 from Hospital Sewage" Antibiotics 8, no. 3: 149. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics8030149

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