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Article

Comparison of Antibiotic Resistance Profile and Biofilm Production of Staphylococcus aureus Isolates Derived from Human Specimens and Animal-Derived Samples

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Laboratorio Genetica dei Microorganismi, Istituto Zooprofilattico Sperimentale della Sicilia, 90129 Palermo, Italy
2
Dipartimento Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche, Viale delle Scienze, University of Palermo, 90128 Palermo, Italy
3
Laboratorio Analisi Baiata srl, via Capitano Francesco Sieli, 1, 91100 Trapani (TP), Italy
*
Authors to whom correspondence should be addressed.
Received: 30 May 2019 / Revised: 3 July 2019 / Accepted: 17 July 2019 / Published: 19 July 2019
(This article belongs to the Special Issue Staphylococci Antimicrobial Resistance)
Background: The diffusion of antimicrobial resistance is a significant concern for public health worldwide. Staphylococcus aureus represents a paradigm microorganism for antibiotic resistance in that resistant strains appear within a decade after the introduction of new antibiotics. Methods: Fourteen S. aureus isolates from human specimens and twenty-one from samples of animal origin, were compared for their antimicrobial resistance and biofilm capability. In addition, they were characterized at the molecular level to detect the antimicrobial resistance mecA gene and genes related with enterotoxin, toxin, and biofilm production. Results: Both phenotypic and molecular analysis showed main differences among human- and animal-derived isolates. Among the human-derived isolates, more multidrug-resistant isolates were detected and mecA gene, enterotoxin, and toxin genes were more prevalent. Different genes involved in biofilm production were detected with bap present only in animal-derived isolates and sasC present in both isolates, however, with a higher prevalence in the human-derived isolates. Biofilm capability was higher in human-derived isolates mainly associated to the sasC gene. Conclusions: The overall results indicate that human S. aureus isolates are more virulent and resistant than the isolates of animal origin randomly selected with no infection anamnesis. This study confirms that selection for more virulent and resistant S. aureus strains is related to the clinical practice. View Full-Text
Keywords: Staphylococcus aureus; Staphylococcal toxins; mecA; antibiotic resistance; biofilm activity; MRSA Staphylococcus aureus; Staphylococcal toxins; mecA; antibiotic resistance; biofilm activity; MRSA
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MDPI and ACS Style

Vitale, M.; Galluzzo, P.; Buffa, P.G.; Carlino, E.; Spezia, O.; Alduina, R. Comparison of Antibiotic Resistance Profile and Biofilm Production of Staphylococcus aureus Isolates Derived from Human Specimens and Animal-Derived Samples. Antibiotics 2019, 8, 97. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics8030097

AMA Style

Vitale M, Galluzzo P, Buffa PG, Carlino E, Spezia O, Alduina R. Comparison of Antibiotic Resistance Profile and Biofilm Production of Staphylococcus aureus Isolates Derived from Human Specimens and Animal-Derived Samples. Antibiotics. 2019; 8(3):97. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics8030097

Chicago/Turabian Style

Vitale, Maria, Paola Galluzzo, Patrizia G. Buffa, Eleonora Carlino, Orazio Spezia, and Rosa Alduina. 2019. "Comparison of Antibiotic Resistance Profile and Biofilm Production of Staphylococcus aureus Isolates Derived from Human Specimens and Animal-Derived Samples" Antibiotics 8, no. 3: 97. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics8030097

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