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Article

UPLC-MS-ESI-QTOF Analysis and Antifungal Activity of the Spondias tuberosa Arruda Leaf and Root Hydroalcoholic Extracts

1
Department of Biological Chemistry, Regional University of Cariri-URCA, Col Antonio Luis, 1161, 63105-000 Pimenta, Crato-CE, Brazil
2
Brejo Santo Campus, Federal University of Cariri-UFCA, R. Olegario Emidio de Araujo, s/n, 63260-000 Centro, Brejo Santo-CE, Brazil
3
Embrapa Tropical Agroindustry, R. Pernambuco, 2270, 60511-110 Pici, Fortaleza-CE, Brazil
4
State University of Ceara-Tauá Campus, BR-020-Bezerra de Sousa, 63660-000 Tauá-CE, Brazil
5
Department of Agricultural and Environmental Sciences, Milan State University, via G. Celoria 2, 20133 Milan, Italy
*
Author to whom correspondence should be addressed.
Received: 8 November 2019 / Revised: 21 November 2019 / Accepted: 23 November 2019 / Published: 28 November 2019
(This article belongs to the Special Issue Antimicrobial Plant Extracts and Phytochemicals)
The aim of this study was to identify and evaluate the chemical compositions and effects of the S. tuberosa leaf and root hydroalcoholic extracts (HELST and HERST) against different strains of Candida. Chemical analysis was performed by Ultra-Performance Liquid Chromatography Coupled to Quadrupole/Time of Flight System (UPLC-MS-ESI-QTOF). The Inhibitory Concentration of 50% of the growth (IC50) as well as the intrinsic and combined action of the extracts with the antifungal fluconazole (FCZ) were determined by the microdilution method while the minimum fungicidal concentrations (MFCs) and the effect on fungal morphological transitions were analyzed by subculture and in humid chambers, respectively. From the preliminary phytochemical analysis, the phenols and flavonoids were the most abundant. The intrinsic IC50 values for HELST ranged from 5716.3 to 7805.8 µg/mL and from 6175.4 to 51070.9 µg/mL for the HERST, whereas the combination of the extracts with fluconazole presented IC50 values from 2.65 to 278.41 µg/mL. The MFC of the extracts, individually, for all the tested strains was ≥16384 µg/mL. When fluconazole was combined with each extract, the MFC against CA URM 5974 was reduced (HELST: 2048 and HERST: 4096 µg/mL). Synergism was observed against standard C. albicans (CA) and C. tropicalis (CT) strains and with the root extract against the CT isolate. The leaf extract inhibited the morphological transition of all strains while the root extract inhibited only CT strains. View Full-Text
Keywords: chromatography; fluconazole; Candida spp., morphological transition chromatography; fluconazole; Candida spp., morphological transition
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MDPI and ACS Style

Thassya Lucas dos Santos, A.; Pereira Carneiro, J.N.; Pereira da Cruz, R.; Lima Sales, D.; Cosmo Andrade, J.; de Oliveira Almeida, W.; Martins da Costa, J.G.; Riceli Vasconcelos Ribeiro, P.; Sousa de Brito, E.; Alves Batista, F.L.; Alves Magalhães, F.E.; Iriti, M.; Morais-Braga, M.F.B.; Coutinho, H.D.M. UPLC-MS-ESI-QTOF Analysis and Antifungal Activity of the Spondias tuberosa Arruda Leaf and Root Hydroalcoholic Extracts. Antibiotics 2019, 8, 240. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics8040240

AMA Style

Thassya Lucas dos Santos A, Pereira Carneiro JN, Pereira da Cruz R, Lima Sales D, Cosmo Andrade J, de Oliveira Almeida W, Martins da Costa JG, Riceli Vasconcelos Ribeiro P, Sousa de Brito E, Alves Batista FL, Alves Magalhães FE, Iriti M, Morais-Braga MFB, Coutinho HDM. UPLC-MS-ESI-QTOF Analysis and Antifungal Activity of the Spondias tuberosa Arruda Leaf and Root Hydroalcoholic Extracts. Antibiotics. 2019; 8(4):240. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics8040240

Chicago/Turabian Style

Thassya Lucas dos Santos, Antonia, Joara N. Pereira Carneiro, Rafael Pereira da Cruz, Débora Lima Sales, Jacqueline Cosmo Andrade, Waltécio de Oliveira Almeida, José G. Martins da Costa, Paulo Riceli Vasconcelos Ribeiro, Edy Sousa de Brito, Francisco L. Alves Batista, Francisco E. Alves Magalhães, Marcello Iriti, Maria F.B. Morais-Braga, and Henrique D.M. Coutinho 2019. "UPLC-MS-ESI-QTOF Analysis and Antifungal Activity of the Spondias tuberosa Arruda Leaf and Root Hydroalcoholic Extracts" Antibiotics 8, no. 4: 240. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics8040240

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