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Open AccessArticle

Antibacterial Activity of Colloidal Silver against Gram-Negative and Gram-Positive Bacteria

1
Clinical Unit of Infectious Diseases, Microbiology and Preventive Medicine, University Hospital Virgen del Rocío, 41013 Seville, Spain
2
Institute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocío, CSIC, University of Seville, 41013 Seville, Spain
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 5 December 2019 / Revised: 10 January 2020 / Accepted: 14 January 2020 / Published: 19 January 2020
(This article belongs to the Special Issue Drugs Repurposing for the Treatment of Bacterial Infections)
Due to the emergence of antimicrobial resistance, new alternative therapies are needed. Silver was used to treat bacterial infections since antiquity due to its known antimicrobial properties. Here, we aimed to evaluate the in vitro activity of colloidal silver (CS) against multidrug-resistant (MDR) Gram-negative and Gram-positive bacteria. A total of 270 strains (Acinetobacter baumannii (n = 45), Pseudomonas aeruginosa (n = 25), Escherichia coli (n = 79), Klebsiella pneumoniae (n = 58)], Staphylococcus aureus (n = 34), Staphylococcus epidermidis (n = 14), and Enterococcus species (n = 15)) were used. The minimal inhibitory concentration (MIC) of CS was determined for all strains by using microdilution assay, and time–kill curve assays of representative reference and MDR strains of these bacteria were performed. Membrane permeation and bacterial reactive oxygen species (ROS) production were determined in presence of CS. CS MIC90 was 4–8 mg/L for all strains. CS was bactericidal, during 24 h, at 1× and 2× MIC against Gram-negative bacteria, and at 2× MIC against Gram-positive bacteria, and it did not affect their membrane permeabilization. Furthermore, we found that CS significantly increased the ROS production in Gram-negative with respect to Gram-positive bacteria at 24 h of incubation. Altogether, these results suggest that CS could be an effective treatment for infections caused by MDR Gram-negative and Gram-positive bacteria. View Full-Text
Keywords: multidrug-resistant bacteria; colloidal silver; Gram-negative bacteria; Gram-positive bacteria multidrug-resistant bacteria; colloidal silver; Gram-negative bacteria; Gram-positive bacteria
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MDPI and ACS Style

Vila Domínguez, A.; Ayerbe Algaba, R.; Miró Canturri, A.; Rodríguez Villodres, Á.; Smani, Y. Antibacterial Activity of Colloidal Silver against Gram-Negative and Gram-Positive Bacteria. Antibiotics 2020, 9, 36. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9010036

AMA Style

Vila Domínguez A, Ayerbe Algaba R, Miró Canturri A, Rodríguez Villodres Á, Smani Y. Antibacterial Activity of Colloidal Silver against Gram-Negative and Gram-Positive Bacteria. Antibiotics. 2020; 9(1):36. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9010036

Chicago/Turabian Style

Vila Domínguez, Andrea; Ayerbe Algaba, Rafael; Miró Canturri, Andrea; Rodríguez Villodres, Ángel; Smani, Younes. 2020. "Antibacterial Activity of Colloidal Silver against Gram-Negative and Gram-Positive Bacteria" Antibiotics 9, no. 1: 36. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9010036

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