Next Article in Journal
Ceftolozane/Tazobactam and Ceftazidime/Avibactam for Multidrug-Resistant Gram-Negative Infections in Immunocompetent Patients: A Single-Center Retrospective Study
Next Article in Special Issue
Benzoxazole-Based Metal Complexes to Reverse Multidrug Resistance in Bacteria
Previous Article in Journal
A Cluster of Candida auris Blood Stream Infections in a Tertiary Care Hospital in Oman from 2016 to 2019
Previous Article in Special Issue
Chalcones Isolated from Arrabidaea brachypoda Flowers as Inhibitors of NorA and MepA Multidrug Efflux Pumps of Staphylococcus aureus
Article

Determination of Drug Efflux Pump Efficiency in Drug-Resistant Bacteria Using MALDI-TOF MS

1
Department of Food Science, National Taiwan Ocean University, No. 2, Pei-Ning Road, Keelung 202, Taiwan
2
Department of Bioscience and Biotechnology, National Taiwan Ocean University, No. 2, Pei-Ning Road, Keelung 202, Taiwan
3
Center of Excellence for the Oceans, National Taiwan Ocean University, No. 2, Pei-Ning Road, Keelung 202, Taiwan
4
Institute of Biochemistry and Molecular Biology, National Yang Ming University, No. 155, Sec. 2, Linong Street, Taipei 112, Taiwan
*
Authors to whom correspondence should be addressed.
Received: 8 July 2020 / Revised: 18 September 2020 / Accepted: 22 September 2020 / Published: 24 September 2020
(This article belongs to the Special Issue The Role of Efflux Pump Inhibitor in Bacterial Multidrug Resistance)
Multidrug efflux pumps play an essential role in antibiotic resistance. The conventional methods, including minimum inhibitory concentration and fluorescent assays, to monitor transporter efflux activity might have some drawbacks, such as indirect evidence or interference from color molecules. In this study, MALDI-TOF MS use was explored for monitoring drug efflux by a multidrug transporter, and the results were compared for validation with the data from conventional methods. Minimum inhibitory concentration was used first to evaluate the activity of Escherichia coli drug transporter AcrB, and this analysis showed that the E. coli overexpressing AcrB exhibited elevated resistance to various antibiotics and dyes. Fluorescence-based studies indicated that AcrB in E. coli could decrease the accumulation of intracellular dyes and display various efflux rate constants for different dyes, suggesting AcrB’s efflux activity. The MALDI-TOF MS analysis parameters were optimized to maintain a detection accuracy for AcrB’s substrates; furthermore, the MS data showed that E. coli overexpressing AcrB led to increased ions abundancy of various dyes and drugs in the extracellular space at different rates over time, illustrating continuous substrate efflux by AcrB. This study concluded that MALDI-TOF MS is a reliable method that can rapidly determine the drug pump efflux activity for various substrates. View Full-Text
Keywords: AcrB; drug resistance; efflux activity; MALDI-TOF MS; multidrug transporter AcrB; drug resistance; efflux activity; MALDI-TOF MS; multidrug transporter
Show Figures

Graphical abstract

MDPI and ACS Style

Lu, W.-J.; Lin, H.-J.; Hsu, P.-H.; Lin, H.-T.V. Determination of Drug Efflux Pump Efficiency in Drug-Resistant Bacteria Using MALDI-TOF MS. Antibiotics 2020, 9, 639. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9100639

AMA Style

Lu W-J, Lin H-J, Hsu P-H, Lin H-TV. Determination of Drug Efflux Pump Efficiency in Drug-Resistant Bacteria Using MALDI-TOF MS. Antibiotics. 2020; 9(10):639. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9100639

Chicago/Turabian Style

Lu, Wen-Jung, Hsuan-Ju Lin, Pang-Hung Hsu, and Hong-Ting V. Lin 2020. "Determination of Drug Efflux Pump Efficiency in Drug-Resistant Bacteria Using MALDI-TOF MS" Antibiotics 9, no. 10: 639. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9100639

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop