Next Article in Journal
A Pilot Study in Sweden on Efficacy of Benzylpenicillin, Oxytetracycline, and Florfenicol in Treatment of Acute Undifferentiated Respiratory Disease in Calves
Next Article in Special Issue
Development of Resistance in Escherichia coli ATCC25922 under Exposure of Sub-Inhibitory Concentration of Olaquindox
Previous Article in Journal
Phage Diversity for Research and Application
Previous Article in Special Issue
Prevalence and Epidemiology of Multidrug-Resistant Pathogens in the Food Chain and the Urban Environment in Northwestern Germany
Due to scheduled maintenance work on our core network, there may be short service disruptions on this website between 16:00 and 16:30 CEST on September 25th.
Article

Pathogenic Escherichia coli Possess Elevated Growth Rates under Exposure to Sub-Inhibitory Concentrations of Azithromycin

1
The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City Q5, Vietnam
2
Division of Aquacultural Biotechnology, Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City Q5, Vietnam
3
Department of Medicine, Cambridge Institute of Therapeutic Immunology & Infectious Disease (CITIID), University of Cambridge, Cambridge CB2 0AW, UK
4
Saw Swee Hock School of Public Health, National University of Singapore, Singapore 117549, Singapore
*
Author to whom correspondence should be addressed.
Received: 26 August 2020 / Revised: 19 October 2020 / Accepted: 26 October 2020 / Published: 26 October 2020
(This article belongs to the Special Issue Antimicrobial Resistance: The Final Frontier)
Antimicrobial resistance (AMR) has been identified by the World Health Organization (WHO) as one of the ten major threats to global health. Advances in technology, including whole-genome sequencing, have provided new insights into the origin and mechanisms of AMR. However, our understanding of the short-term impact of antimicrobial pressure and resistance on the physiology of bacterial populations is limited. We aimed to investigate morphological and physiological responses of clinical isolates of E. coli under short-term exposure to key antimicrobials. We performed whole-genome sequencing on twenty-seven E. coli isolates isolated from children with sepsis to evaluate their AMR gene content. We assessed their antimicrobial susceptibility profile and measured their growth dynamics and morphological characteristics under exposure to varying concentrations of ciprofloxacin, ceftriaxone, tetracycline, gentamicin, and azithromycin. AMR was common, with all organisms resistant to at least one antimicrobial; a total of 81.5% were multi-drug-resistant (MDR). We observed an association between resistance profile and morphological characteristics of the E. coli over a three-hour exposure to antimicrobials. Growth dynamics experiments demonstrated that resistance to tetracycline promoted the growth of E. coli under antimicrobial-free conditions, while resistance to the other antimicrobials incurred a fitness cost. Notably, antimicrobial exposure heterogeneously suppressed bacterial growth, but sub-MIC concentrations of azithromycin increased the maximum growth rate of the clinical isolates. Our results outline complex interactions between organism and antimicrobials and raise clinical concerns regarding exposure of sub-MIC concentrations of specific antimicrobials. View Full-Text
Keywords: Escherichia coli; azithromycin; tetracycline; growth rate; fitness cost; antimicrobial resistance; resistome; morphology; physiology Escherichia coli; azithromycin; tetracycline; growth rate; fitness cost; antimicrobial resistance; resistome; morphology; physiology
Show Figures

Figure 1

MDPI and ACS Style

Tuan-Anh, T.; Tuyen, H.T.; Minh Chau, N.N.; Toan, N.D.; Triet, T.H.; Triet, L.M.; Trang, N.H.T.; To, N.T.N.; Bartholdson Scott, J.; The, H.C.; Thanh, D.P.; Clapham, H.; Baker, S. Pathogenic Escherichia coli Possess Elevated Growth Rates under Exposure to Sub-Inhibitory Concentrations of Azithromycin. Antibiotics 2020, 9, 735. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9110735

AMA Style

Tuan-Anh T, Tuyen HT, Minh Chau NN, Toan ND, Triet TH, Triet LM, Trang NHT, To NTN, Bartholdson Scott J, The HC, Thanh DP, Clapham H, Baker S. Pathogenic Escherichia coli Possess Elevated Growth Rates under Exposure to Sub-Inhibitory Concentrations of Azithromycin. Antibiotics. 2020; 9(11):735. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9110735

Chicago/Turabian Style

Tuan-Anh, Tran, Ha T. Tuyen, Nguyen N. Minh Chau, Nguyen D. Toan, Tran H. Triet, Le M. Triet, Nguyen H.T. Trang, Nguyen T.N. To, Josefin Bartholdson Scott, Hao C. The, Duy P. Thanh, Hannah Clapham, and Stephen Baker. 2020. "Pathogenic Escherichia coli Possess Elevated Growth Rates under Exposure to Sub-Inhibitory Concentrations of Azithromycin" Antibiotics 9, no. 11: 735. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9110735

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop