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Open AccessArticle

Analysis of Vancomycin-Resistant Enterococci in Hemato-Oncological Patients

Department of Microbiology, University Hospital Olomouc and Faculty of Medicine and Dentistry, Palacký University Olomouc, 77900 Olomouc, Czech Republic
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Received: 28 September 2020 / Revised: 30 October 2020 / Accepted: 6 November 2020 / Published: 7 November 2020
Enterococci are important bacterial pathogens, and their significance is even greater in the case of vancomycin-resistant enterococci (VRE). The study analyzed the presence of VRE in the gastrointestinal tract (GIT) of hemato-oncological patients. Active screening using selective agars yielded VRE for phenotypic and genotypic analyses. Isolated strains were identified with MALDI-TOF MS, (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry) their susceptibility to antibiotics was tested, and resistance genes (vanA, vanB, vanC-1, vanC2-C3) and genes encoding virulence factors (asa1, gelE, cylA, esp, hyl) were detected. Pulsed-field gel electrophoresis was used to assess the relationship of the isolated strains. Over a period of three years, 103 VanA-type VRE were identified in 1405 hemato-oncological patients. The most frequently detected virulence factor was extracellular surface protein (84%), followed by hyaluronidase (40%). Unique restriction profiles were observed in 33% of strains; clonality was detected in 67% of isolates. The study found that 7% of hemato-oncological patients carried VRE in their GIT. In all cases, the species identified was Enterococcus faecium. No clone persisted for the entire 3-year study period. However, genetically different clusters were observed for shorter periods of time, no longer than eight months, with identical VRE spreading among patients. View Full-Text
Keywords: VRE; GIT; hemato-oncological patients; clonality VRE; GIT; hemato-oncological patients; clonality
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MDPI and ACS Style

Hricová, K.; Štosová, T.; Kučová, P.; Fišerová, K.; Bardoň, J.; Kolář, M. Analysis of Vancomycin-Resistant Enterococci in Hemato-Oncological Patients. Antibiotics 2020, 9, 785. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9110785

AMA Style

Hricová K, Štosová T, Kučová P, Fišerová K, Bardoň J, Kolář M. Analysis of Vancomycin-Resistant Enterococci in Hemato-Oncological Patients. Antibiotics. 2020; 9(11):785. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9110785

Chicago/Turabian Style

Hricová, Kristýna; Štosová, Taťána; Kučová, Pavla; Fišerová, Kateřina; Bardoň, Jan; Kolář, Milan. 2020. "Analysis of Vancomycin-Resistant Enterococci in Hemato-Oncological Patients" Antibiotics 9, no. 11: 785. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9110785

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