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Article

Synergistic Antimicrobial Activity of Supplemented Medical-Grade Honey against Pseudomonas aeruginosa Biofilm Formation and Eradication

1
Small Animal Department, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Ghent, Belgium
2
Department of Dentistry, Orthodontics and Craniofacial Biology, Radboud University Medical Center, Philips van Leydenlaan 25, 6525EX Nijmegen, The Netherlands
3
Dierenkliniek Parkstad, Bautscherweg 56, 6418EM Heerlen, The Netherlands
4
Laboratory of Pharmaceutical Microbiology, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium
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Microbial Biotechnology-Molecular Bacteriology-Virology Laboratory, Department of Biochemistry and Biotechnology, University of Thessaly, Biopolis-Mezurlo, 41500 Larissa, Greece
6
Wound Clinic [email protected], Linksfield Road 45, Dowerglen, Johannesburg 1612, South Africa
7
Triticum Exploitatie BV, Sleperweg 44, 6222NK Maastricht, The Netherlands
*
Author to whom correspondence should be addressed.
Received: 18 November 2020 / Revised: 1 December 2020 / Accepted: 2 December 2020 / Published: 4 December 2020
(This article belongs to the Special Issue Antibiotic Tolerance of Bacterial Biofilms)
Biofilms hinder wound healing. Medical-grade honey (MGH) is a promising therapy because of its broad-spectrum antimicrobial activity and the lack of risk for resistance. This study investigated the inhibitory and eradicative activity against multidrug-resistant Pseudomonas aeruginosa biofilms by different established MGH-based wound care formulations. Six different natural wound care products (Medihoney, Revamil, Mebo, Melladerm, L-Mesitran Ointment, and L-Mesitran Soft) were tested in vitro. Most of them contain MGH only, whereas some were supplemented. L-Mesitran Soft demonstrated the most potent antimicrobial activity (6.08-log inhibition and 3.18-log eradication). Other formulations ranged between 0.89-log and 4.80-log inhibition and 0.65-log and 1.66-log eradication. Therefore, the contribution of different ingredients of L-Mesitran Soft was investigated in more detail. The activity of the same batch of raw MGH (1.38-log inhibition and 2.35-log eradication), vitamins C and E (0.95-log inhibition and 0.94-log eradication), and all ingredients except MGH (1.69-log inhibition and 0.75-log eradication) clearly support a synergistic activity of components within the L-Mesitran Soft formulation. Several presented clinical cases illustrate its clinical antimicrobial efficacy against Pseudomonas aeruginosa biofilms. In conclusion, MGH is a potent treatment for Pseudomonas biofilms. L-Mesitran Soft has the strongest antimicrobial activity, which is likely due to the synergistic activity mediated by its supplements. View Full-Text
Keywords: medical-grade honey; supplements; vitamin C; vitamin E; antimicrobial; biofilm; Pseudomonas aeruginosa; wound medical-grade honey; supplements; vitamin C; vitamin E; antimicrobial; biofilm; Pseudomonas aeruginosa; wound
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MDPI and ACS Style

Pleeging, C.C.F.; Coenye, T.; Mossialos, D.; de Rooster, H.; Chrysostomou, D.; Wagener, F.A.D.T.G.; Cremers, N.A.J. Synergistic Antimicrobial Activity of Supplemented Medical-Grade Honey against Pseudomonas aeruginosa Biofilm Formation and Eradication. Antibiotics 2020, 9, 866. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9120866

AMA Style

Pleeging CCF, Coenye T, Mossialos D, de Rooster H, Chrysostomou D, Wagener FADTG, Cremers NAJ. Synergistic Antimicrobial Activity of Supplemented Medical-Grade Honey against Pseudomonas aeruginosa Biofilm Formation and Eradication. Antibiotics. 2020; 9(12):866. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9120866

Chicago/Turabian Style

Pleeging, Carlos C.F., Tom Coenye, Dimitris Mossialos, Hilde de Rooster, Daniela Chrysostomou, Frank A.D.T.G. Wagener, and Niels A.J. Cremers. 2020. "Synergistic Antimicrobial Activity of Supplemented Medical-Grade Honey against Pseudomonas aeruginosa Biofilm Formation and Eradication" Antibiotics 9, no. 12: 866. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9120866

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