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Article

In vitro Antibiotic and Modulatory Activity of Mesosphaerum suaveolens (L.) Kuntze against Candida strains

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Postgraduate Program in Molecular Bioprospecting, Regional University of Cariri (URCA), Crato 63122-290, CE, Brazil
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Postgraduate Program of Plant Biology, Federal University of Pernambuco, Recife 50670-901, Brazil
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Laboratory of Applied Mycology of Cariri, Regional University of Cariri (URCA), Crato 63122-290, CE, Brazil
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Nursing Course at the Regional University of Cariri (URCA), Crato-CE-Brazil
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Postgraduate Program in Botany – National Amazon Research Institute (INPA), Manaus 69067-375, AM, Brazil
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Biology and Toxicology Laboratory, University of Regional Cariri (URCA), Crato 63122-290, CE, Brazil
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Department of Agricultural and Environmental Sciences, Milan State University, 20133 Milan, Italy
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Vegetable Ecophysiology Laboratory, Regional University of Cariri, Crato 63122-290, CE, Brazil
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Author to whom correspondence should be addressed.
Received: 9 December 2019 / Revised: 20 January 2020 / Accepted: 24 January 2020 / Published: 27 January 2020
(This article belongs to the Special Issue Antimicrobial Plant Extracts and Phytochemicals)
The emergence of fungal resistance to commercial drugs has been a major problem for the WHO. In this context, research with natural products is promising in the discovery of new active substances. Thus, this work evaluated the antifungal effect of a medicinal plant (i.e., Mesosphaerum suaveolens) against strains of the genus Candida, tested the combined effect with the drug fluconazole, and, finally, determined the phenolic constituents present in the species. Initially, aqueous extracts of leaves (AELMs) and aerial parts (AEAPMs) of the species were prepared. For microbiological assays, the minimum fungicidal concentration was determined by broth microdilution, and the combined effect of fluconazole extracts were verified by sub-inhibitory microdilution concentrations (CFM/8) followed by spectrophotometric readings which were used to determine the IC50. HPLC detected the presence of flavonoids and phenolic acids, detecting eight compounds present in the samples of which caffeic acid and quercetin were major components. The AELMs modulated fluconazole activity since it decreased fluconazole’s IC50 from 7.8 µg/mL to an IC50 of 4.7 µg/mL (CA LM 77) and from 28.8 µg/mL to 18.26 µg/mL (CA INCQS 40006) for the C. albicans strains. The AEAPMs were able to potentiate the effect of fluconazole more effectively than the AELMs. Such an effect was significant for the 16 µg/mL concentration for CA LM 77 and 32 µg/mL for CA INCQS 40006. The AEAPMs as well as the AELMs presented clinically relevant activities for C. tropicalis strains. For the C. tropicalis LM 23 strain, the AEPMs obtained an IC50 of 25 µg/mL and the AELMs an IC50 of 359.9 µg/mL. View Full-Text
Keywords: Hyptis suaveolens; Candida; HPLC-DAD; bamburral; phychochemistry Hyptis suaveolens; Candida; HPLC-DAD; bamburral; phychochemistry
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MDPI and ACS Style

Rodrigues Costa, A.; Bezerra, J.W.A.; Pereira da Cruz, R.; de Freitas, M.A.; da Silva, V.B.; Neto, J.C.; dos Santos, A.T.L.; Bezerra Morais Braga, M.F.; da Silva, L.A.; Ivaneide Rocha, M.; Kamdem, J.P.; Iriti, M.; Vitalini, S.; Duarte, A.E.; Barros, L.M. In vitro Antibiotic and Modulatory Activity of Mesosphaerum suaveolens (L.) Kuntze against Candida strains. Antibiotics 2020, 9, 46. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9020046

AMA Style

Rodrigues Costa A, Bezerra JWA, Pereira da Cruz R, de Freitas MA, da Silva VB, Neto JC, dos Santos ATL, Bezerra Morais Braga MF, da Silva LA, Ivaneide Rocha M, Kamdem JP, Iriti M, Vitalini S, Duarte AE, Barros LM. In vitro Antibiotic and Modulatory Activity of Mesosphaerum suaveolens (L.) Kuntze against Candida strains. Antibiotics. 2020; 9(2):46. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9020046

Chicago/Turabian Style

Rodrigues Costa, Adrielle, José W.A. Bezerra, Rafael Pereira da Cruz, Maria A. de Freitas, Viviane B. da Silva, João C. Neto, Antonia T.L. dos Santos, Maria F. Bezerra Morais Braga, Leomara A. da Silva, Maria Ivaneide Rocha, Jean P. Kamdem, Marcello Iriti, Sara Vitalini, Antonia E. Duarte, and Luiz M. Barros 2020. "In vitro Antibiotic and Modulatory Activity of Mesosphaerum suaveolens (L.) Kuntze against Candida strains" Antibiotics 9, no. 2: 46. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9020046

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