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Article

Modified Aminoglycosides Bind Nucleic Acids in High-Molecular-Weight Complexes

1
Department of Microbiology and Center for RNA Biology, The Ohio State University, Columbus, OH 43210-1292, USA
2
Department of Pharmaceutical Sciences in the College of Pharmacy, University of Kentucky, Lexington, KY 40536-0596, USA
*
Author to whom correspondence should be addressed.
Received: 6 February 2020 / Revised: 17 February 2020 / Accepted: 18 February 2020 / Published: 21 February 2020
Aminoglycosides represent a large group of antibiotics well known for their ability to target the bacterial ribosome. In studying 6”-substituted variants of the aminoglycoside tobramycin, we serendipitously found that compounds with C12 or C14 linear alkyl substituents potently inhibit reverse transcription in vitro. Initial observations suggested specific inhibition of reverse transcriptase. However, further analysis showed that these and related compounds bind nucleic acids with high affinity, forming high-molecular weight complexes. Stable complex formation is observed with DNA or RNA in single- or double-stranded form. Given the amphiphilic nature of these aminoglycoside derivatives, they likely form micelles and/or vesicles with surface-bound nucleic acids. Hence, these compounds may be useful tools to localize nucleic acids to surfaces or deliver nucleic acids to cells or organelles. View Full-Text
Keywords: tobramycin; kanamycin; RNA; DNA; reverse transcriptase tobramycin; kanamycin; RNA; DNA; reverse transcriptase
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MDPI and ACS Style

Ying, L.; Zhu, H.; Fosso, M.Y.; Garneau-Tsodikova, S.; Fredrick, K. Modified Aminoglycosides Bind Nucleic Acids in High-Molecular-Weight Complexes. Antibiotics 2020, 9, 93. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9020093

AMA Style

Ying L, Zhu H, Fosso MY, Garneau-Tsodikova S, Fredrick K. Modified Aminoglycosides Bind Nucleic Acids in High-Molecular-Weight Complexes. Antibiotics. 2020; 9(2):93. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9020093

Chicago/Turabian Style

Ying, Lanqing, Hongkun Zhu, Marina Y. Fosso, Sylvie Garneau-Tsodikova, and Kurt Fredrick. 2020. "Modified Aminoglycosides Bind Nucleic Acids in High-Molecular-Weight Complexes" Antibiotics 9, no. 2: 93. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9020093

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