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A Novel, Integron-Regulated, Class C β-Lactamase

Centre for Antibiotic Resistance Research (CARe), University of Gothenburg, Gothenburg, Sweden
Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, 405 30 Gothenburg, Sweden
Author to whom correspondence should be addressed.
Received: 12 February 2020 / Revised: 10 March 2020 / Accepted: 11 March 2020 / Published: 14 March 2020
(This article belongs to the Special Issue Microbial Drug Resistance Genes)
AmpC-type β-lactamases severely impair treatment of many bacterial infections, due to their broad spectrum (they hydrolyze virtually all β-lactams, except fourth-generation cephalosporins and carbapenems) and the increasing incidence of plasmid-mediated versions. The original chromosomal AmpCs are often tightly regulated, and their expression is induced in response to exposure to β-lactams. Regulation of mobile ampC expression is in many cases less controlled, giving rise to constitutively resistant strains with increased potential for development or acquisition of additional resistances. We present here the identification of two integron-encoded ampC genes, blaIDC-1 and blaIDC-2 (integron-derived cephalosporinase), with less than 85% amino acid sequence identity to any previously annotated AmpC. While their resistance pattern identifies them as class C β-lactamases, their low isoelectric point (pI) values make differentiation from other β-lactamases by isoelectric focusing impossible. To the best of our knowledge, this is the first evidence of an ampC gene cassette within a class 1 integron, providing a mobile context with profound potential for transfer and spread into clinics. It also allows bacteria to adapt expression levels, and thus reduce fitness costs, e.g., by cassette-reshuffling. Analyses of public metagenomes, including sewage metagenomes, show that the discovered ampCs are primarily found in Asian countries. View Full-Text
Keywords: Class C β-lactamase; integron; functional metagenomics; antibiotic resistance Class C β-lactamase; integron; functional metagenomics; antibiotic resistance
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MDPI and ACS Style

Böhm, M.-E.; Razavi, M.; Flach, C.-F.; Larsson, D.G.J. A Novel, Integron-Regulated, Class C β-Lactamase. Antibiotics 2020, 9, 123.

AMA Style

Böhm M-E, Razavi M, Flach C-F, Larsson DGJ. A Novel, Integron-Regulated, Class C β-Lactamase. Antibiotics. 2020; 9(3):123.

Chicago/Turabian Style

Böhm, Maria-Elisabeth, Mohammad Razavi, Carl-Fredrik Flach, and D. G.J. Larsson. 2020. "A Novel, Integron-Regulated, Class C β-Lactamase" Antibiotics 9, no. 3: 123.

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