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Article

Effects of Antibiotics on the Intestinal Microbiota of Mice

1
Department of Clinical Microbiology, Hvidovre University Hospital, 2650 Copenhagen, Denmark
2
Department of Clinical Microbiology, Slagelse Hospital, 4200 Slagelse, Denmark
3
Department of Medical Microbiology & Infection Control, University Medical Centers, Location VUmc, 1081HV Amsterdam, The Netherlands
4
In Biome, Science Park, 116, 1081XG Amsterdam, The Netherlands
5
Department of Medical Microbiology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University medical Center, 6202 AZ Maastricht, The Netherlands
6
Department of Biology, University of Copenhagen, 2200 Copenhagen, Denmark
7
Department of Clinical Microbiology, Rigshospitalet, 2100 Copenhagen, Denmark
*
Author to whom correspondence should be addressed.
Received: 17 February 2020 / Revised: 14 April 2020 / Accepted: 15 April 2020 / Published: 17 April 2020
Studies on human and mouse gastrointestinal microbiota have correlated the composition of the microbiota to a variety of diseases, as well as proved it vital to prevent colonization with resistant bacteria, a phenomenon known as colonization resistance. Antibiotics dramatically modify the gut community and there are examples of how antibiotic usage lead to colonization with resistant bacteria [e.g., dicloxacillin usage selecting for ESBL-producing E. coli carriage], as shown by Hertz et al. Here, we investigated the impact of five antibiotics [cefotaxime, cefuroxime, dicloxacillin, clindamycin, and ciprofloxacin] on the intestinal microbiota in mice. Five different antibiotics were each given to groups of five mice. The intestinal microbiotas were profiled by use of the IS-pro analysis; a 16S–23S rDNA interspace [IS]-region-based profiling method. For the mice receiving dicloxacillin and clindamycin, we observed dramatic shifts in dominating phyla from day 1 to day 5. Of note, diversity increased, but overall bacterial load decreased. For ciprofloxacin, cefotaxime, and cefuroxime there were few overall changes. We speculate that antibiotics with efficacy against the abundant anaerobes in the gut, particularly Bacteroidetes, can in fact be selected for resistant bacteria, disregarding the spectrum of activity. View Full-Text
Keywords: intestinal microbiota; 16S; IS-pro; antibiotics; dicloxacillin intestinal microbiota; 16S; IS-pro; antibiotics; dicloxacillin
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MDPI and ACS Style

Hertz, F.B.; Budding, A.E.; van der Lugt-Degen, M.; Savelkoul, P.H.; Løbner-Olesen, A.; Frimodt-Møller, N. Effects of Antibiotics on the Intestinal Microbiota of Mice. Antibiotics 2020, 9, 191. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9040191

AMA Style

Hertz FB, Budding AE, van der Lugt-Degen M, Savelkoul PH, Løbner-Olesen A, Frimodt-Møller N. Effects of Antibiotics on the Intestinal Microbiota of Mice. Antibiotics. 2020; 9(4):191. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9040191

Chicago/Turabian Style

Hertz, Frederik B.; Budding, Andries E.; van der Lugt-Degen, Malieka; Savelkoul, Paul H.; Løbner-Olesen, Anders; Frimodt-Møller, Niels. 2020. "Effects of Antibiotics on the Intestinal Microbiota of Mice" Antibiotics 9, no. 4: 191. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9040191

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