Evaluation of Multiple Impacts of Furfural Acetone on Nematodes In Vitro and Control Efficiency against Root-Knot Nematodes in Pots and Fields
Abstract
:1. Introduction
2. Results
2.1. Effect of FAc on the Egg Hatching of M. incognita
2.2. Effect of FAc on the Reproduction, Feeding, and Growth of C. elegans
2.3. Efficiency of FAc against RKNs in Pots
2.4. Efficiency of FAc against RKNs in the Field
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Nematodes
4.3. Effect of FAc on the Egg Hatching of M. incognita
4.4. Effect of FAc on the Reproduction of C. elegans
4.5. Effect of FAc on Pharyngeal Pumping of C. elegans
4.6. Effect of FAc on the Growth of C. elegans
4.7. Control Efficacy of FAc against RKNs in Pots
4.8. Control Efficacy of FAc against RKNs in Field
4.9. Statistical Analyses
Author Contributions
Funding
Conflicts of Interest
References
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Concentration (mg/L) | 2 Days | 4 Days | ||
---|---|---|---|---|
Hatched Worms/Egg Mass | Inhibition Rate (%) | Hatched Worms/Egg Mass | Inhibition Rate (%) | |
0 | 47.6 ± 5.8 a 1 | (0.0) | 74.3 ± 9.3 a | (0.0) |
50 | 40.1 ± 4.9 a | 15.8 | 67.8 ± 10.1 a | 8.8 |
100 | 16.9 ± 3.3 b | 64.6 | 19.7 ± 5.3 b | 73.5 |
200 | 4.0 ± 1.5 c | 91.7 | 4.6 ± 1.5 b | 93.8 |
500 | 2.4 ± 0.6 c | 94.9 | 2.7 ± 0.7 b | 96.4 |
Concentrations (mg/L) | Pumping 1 (Hz) | |
---|---|---|
24 h | 48 h | |
0 | 183.7 ± 2.6 a 2 | 175.1 ± 3.9 a |
25 | 163.7 ± 8.8 ab | 155.4 ± 2.7 b |
50 | 164.3 ± 7.8 ab | 147.9 ± 4.5 b |
100 | 148.1 ± 7.8 b | 135.5 ± 4.5 c |
200 | 117.8 ± 2.9 c | 109.1 ± 3.5 d |
Treatment | Disease Index | Control Effect (%) | Number of RKNs/30 g Soil | Reduction Rate of RKNs (%) |
---|---|---|---|---|
Irrigation 1 | ||||
Control 2 | 82.4 ± 2.2 b 7 | (0.0) | 432 ± 93 a | (0.0) |
Fac 3 10 mg/plant | 57.5 ± 2.8 a | 30.2 | 246 ± 62 a | 43.1 |
FAc 50 mg/plant | 52.3 ± 2.9 a | 36.9 | 225 ± 97 a | 47.9 |
Ave 4 10 mg/plant | 58.1 ± 3.0 a | 29.5 | 401 ± 31 a | 7.2 |
Fumigant 5 | ||||
Control | 73.6 ± 2.6 b | (0.0) | 1617 ± 1323 a | (0.0) |
FAc 375 mg/m2 | 61.1 ± 3.8 a | 17.1 | 759 ± 259 a | 53.1 |
FAc 750 mg/m2 | 59.0 ± 2.6 a | 19.9 | 400 ± 51 a | 75.3 |
MSo 6 750 mg/m2 | 65.4 ± 3.2 a | 11.2 | 726 ± 314 a | 55.1 |
Treatment | Fresh Weight on the Ground (g) | Plant Height (cm) | Stem Thickness (cm) |
---|---|---|---|
Irrigation | |||
Control | 618 ± 78 a 1 | 89.5 ± 3.1 a | 3.64 ± 0.13 a |
FAc 10 mg/plant | 737 ± 109 a | 96.5 ± 3.7 a | 3.76 ± 0.11 a |
FAc 50 mg/plant | 593 ± 72 a | 85.3 ± 4.4 a | 3.59 ± 0.14 a |
Ave 10 mg/plant | 655 ± 16 a | 94.7 ± 2.8 a | 3.72 ± 0.10 a |
Fumigant | |||
Control | 667 ± 52 a | 101.0 ± 3.0 a | 3.46 ± 0.09 a |
FAc 375 mg/m2 | 724 ± 77 a | 98.7 ± 3.4 a | 3.70 ± 0.15 a |
FAc 750 mg/m2 | 749 ± 44 a | 105.4 ± 2.9 a | 3.71 ± 0.11 a |
MSo 750 mg/m2 | 821 ± 42 a | 106.1 ± 2.7 a | 3.63 ± 0.08 a |
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Cheng, W.; Yang, X.; Zeng, L.; Huang, D.; Cai, M.; Zheng, L.; Yu, Z.; Zhang, J. Evaluation of Multiple Impacts of Furfural Acetone on Nematodes In Vitro and Control Efficiency against Root-Knot Nematodes in Pots and Fields. Antibiotics 2020, 9, 605. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9090605
Cheng W, Yang X, Zeng L, Huang D, Cai M, Zheng L, Yu Z, Zhang J. Evaluation of Multiple Impacts of Furfural Acetone on Nematodes In Vitro and Control Efficiency against Root-Knot Nematodes in Pots and Fields. Antibiotics. 2020; 9(9):605. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9090605
Chicago/Turabian StyleCheng, Wanli, Xue Yang, Li Zeng, Dian Huang, Minmin Cai, Longyu Zheng, Ziniu Yu, and Jibin Zhang. 2020. "Evaluation of Multiple Impacts of Furfural Acetone on Nematodes In Vitro and Control Efficiency against Root-Knot Nematodes in Pots and Fields" Antibiotics 9, no. 9: 605. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9090605