Next Article in Journal
Measuring Antibiotic Stewardship Programmes and Initiatives: An Umbrella Review in Primary Care Medicine and a Systematic Review of Dentistry
Previous Article in Journal
Evaluation of Multiple Impacts of Furfural Acetone on Nematodes In Vitro and Control Efficiency against Root-Knot Nematodes in Pots and Fields
Open AccessArticle

UHPLC-ESI-QTOF-MS/MS-Based Molecular Networking Guided Isolation and Dereplication of Antibacterial and Antifungal Constituents of Ventilago denticulata

1
Chulabhorn Graduate Institute, Chemical Biology Program, Chulabhorn Royal Academy, Laksi, Bangkok 10210, Thailand
2
School of Science, Mae Fah Luang University, Muang, Chiang Rai 57100, Thailand
3
Center of Chemical Innovation for Sustainability (CIS), Mae Fah Luang University, Muang, Chiang Rai 57100, Thailand
4
Chulabhorn Research Institute, Kamphaeng Phet 6 Road, Laksi, Bangkok 10210, Thailand
5
Center of Excellence on Environmental Health and Toxicology (EHT), CHE, Ministry of Education, Bangkok 10210, Thailand
*
Author to whom correspondence should be addressed.
Received: 6 August 2020 / Revised: 11 September 2020 / Accepted: 12 September 2020 / Published: 15 September 2020
Ventilago denticulata is an herbal medicine for the treatment of wound infection; therefore this plant may rich in antibacterial agents. UHPLC-ESI-QTOF-MS/MS-Based molecular networking guided isolation and dereplication led to the identification of antibacterial and antifungal agents in V. denticulata. Nine antimicrobial agents in V. denticulata were isolated and characterized; they are divided into four groups including (I) flavonoid glycosides, rhamnazin 3-rhamninoside (7), catharticin or rhamnocitrin 3-rhamninoside (8), xanthorhamnin B or rhamnetin 3-rhamninoside (9), kaempferol 3-rhamninoside (10) and flavovilloside or quercetin 3-rhamninoside (11), (II) benzisochromanquinone, ventilatones B (12) and A (15), (III) a naphthopyrone ventilatone C (16) and (IV) a triterpene lupeol (13). Among the isolated compounds, ventilatone C (16) was a new compound. Moreover, kaempferol, chrysoeriol, isopimpinellin, rhamnetin, luteolin, emodin, rhamnocitrin, ventilagodenin A, rhamnazin and mukurozidiol, were tentatively identified as antimicrobial compounds in extracts of V. denticulata by a dereplication method. MS fragmentation of rhamnose-containing compounds gave an oxonium ion, C6H9O3+ at m/z 129, while that of galactose-containing glycosides provided the fragment ion at m/z 163 of C6H11O5+. These fragment ions may be used to confirm the presence of rhamnose or galactose in mass spectrometry-based analysis of natural glycosides or oligosaccharide attached to biomolecules, that is, glycoproteins. View Full-Text
Keywords: Ventilago denticulata; natural products; antibacterial activity; antifungal activity; dereplication; molecular networking; flavonoid glycosides; mass spectrometry; MS fragmentation of sugar Ventilago denticulata; natural products; antibacterial activity; antifungal activity; dereplication; molecular networking; flavonoid glycosides; mass spectrometry; MS fragmentation of sugar
Show Figures

Graphical abstract

MDPI and ACS Style

Azizah, M.; Pripdeevech, P.; Thongkongkaew, T.; Mahidol, C.; Ruchirawat, S.; Kittakoop, P. UHPLC-ESI-QTOF-MS/MS-Based Molecular Networking Guided Isolation and Dereplication of Antibacterial and Antifungal Constituents of Ventilago denticulata. Antibiotics 2020, 9, 606. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9090606

AMA Style

Azizah M, Pripdeevech P, Thongkongkaew T, Mahidol C, Ruchirawat S, Kittakoop P. UHPLC-ESI-QTOF-MS/MS-Based Molecular Networking Guided Isolation and Dereplication of Antibacterial and Antifungal Constituents of Ventilago denticulata. Antibiotics. 2020; 9(9):606. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9090606

Chicago/Turabian Style

Azizah, Muhaiminatul; Pripdeevech, Patcharee; Thongkongkaew, Tawatchai; Mahidol, Chulabhorn; Ruchirawat, Somsak; Kittakoop, Prasat. 2020. "UHPLC-ESI-QTOF-MS/MS-Based Molecular Networking Guided Isolation and Dereplication of Antibacterial and Antifungal Constituents of Ventilago denticulata" Antibiotics 9, no. 9: 606. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9090606

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop