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Brief Report

Uropathogenic Escherichia coli Biofilm-Forming Capabilities are not Predictable from Clinical Details or from Colonial Morphology

1
Department of Biological Sciences, Cork Institute of Technology, Bishopstown, T12P928 Cork, Ireland
2
Department of Clinical Microbiology, Cork University Hospital, Wilton, T12 DC4A Cork, Ireland
3
Department of Biological Sciences, Galway-Mayo Institute of Technology, Old Dublin Road, H91 DCH9 Galway, Ireland
*
Author to whom correspondence should be addressed.
Received: 18 March 2020 / Revised: 28 April 2020 / Accepted: 29 April 2020 / Published: 30 April 2020
(This article belongs to the Special Issue Chronic and Infectious Diseases)
Antibiotic resistance is increasing to an extent where efficacy is not guaranteed when treating infection. Biofilm formation has been shown to complicate treatment, whereby the formation of biofilm is associated with higher minimum inhibitory concentration values of antibiotic. The objective of the current paper was to determine whether biofilm formation is variable among uropathogenic Escherichia coli isolates and whether formation is associated with recurrent urinary tract infection (UTI), and whether it can be predicted by phenotypic appearance on culture medium A total of 62 E. coli isolates that were reported as the causative agent of UTI were studied (33 from patients denoted as having recurrent UTI and 29 from patients not specified as having recurrent UTI). The biofilm forming capability was determined using a standard microtitre plate method, using E. coli ATCC 25922 as the positive control. The majority of isolates (93.6%) were found to be biofilm formers, whereby 81% were denoted as strong or very strong producers of biofilm when compared to the positive control. Through the use of a Wilcox test, the difference in biofilm forming propensity between the two patient populations was found to not be statistically significant (p = 0.5). Furthermore, it was noted that colony morphology was not a reliable predictor of biofilm-forming propensity. The findings of this study indicate that biofilm formation is very common among uropathogens, and they suggest that the biofilm-forming capability might be considered when treating UTI. Clinical details indicating a recurrent infection were not predictors of biofilm formation. View Full-Text
Keywords: uropathogen; Escherichia coli; biofilm formation; colony morphology; urinary tract infection uropathogen; Escherichia coli; biofilm formation; colony morphology; urinary tract infection
MDPI and ACS Style

Whelan, S.; O’Grady, M.C.; Corcoran, D.; Finn, K.; Lucey, B. Uropathogenic Escherichia coli Biofilm-Forming Capabilities are not Predictable from Clinical Details or from Colonial Morphology. Diseases 2020, 8, 11. https://0-doi-org.brum.beds.ac.uk/10.3390/diseases8020011

AMA Style

Whelan S, O’Grady MC, Corcoran D, Finn K, Lucey B. Uropathogenic Escherichia coli Biofilm-Forming Capabilities are not Predictable from Clinical Details or from Colonial Morphology. Diseases. 2020; 8(2):11. https://0-doi-org.brum.beds.ac.uk/10.3390/diseases8020011

Chicago/Turabian Style

Whelan, Shane, Mary C. O’Grady, Dan Corcoran, Karen Finn, and Brigid Lucey. 2020. "Uropathogenic Escherichia coli Biofilm-Forming Capabilities are not Predictable from Clinical Details or from Colonial Morphology" Diseases 8, no. 2: 11. https://0-doi-org.brum.beds.ac.uk/10.3390/diseases8020011

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