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Communication

Preliminary Protocol Development of a HPLC-TBARS-EVSC (Ex Vivo Stratum Corneum) Assay for Skin Research: Application in a Sunscreen System

1
Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-900, Brazil
2
Universidad Nacional de Trujillo Juan Pablo II Av. s/n, Trujillo 13011, Peru
*
Author to whom correspondence should be addressed.
Academic Editor: Susi Burgalassi
Received: 15 March 2021 / Revised: 6 April 2021 / Accepted: 25 April 2021 / Published: 27 April 2021
(This article belongs to the Special Issue Functional Ingredients in Topical and Transdermal Delivery Systems)
Considering the importance of the cutaneous tissue investigation and the need for the development of new protocols to non-invasively establish the safety and efficacy of dermocosmetics and topical products, we aimed at developing an HPLC-TBARS-EVSC (high performance liquid chromatography–thiobarbituric acid reactive species–ex vivo stratum corneum) assay for the lipid peroxidation measurement on subjects’ stratum corneum (SC) obtained by tape stripping; additionally, we applied the HPLC-TBARS-EVSC assay in an emulsified sunscreen system containing ethylhexyl triazone and bemotrizinol as UV filters. HPLC analysis was performed in isocratic mode with 35% methanol/65% phosphate buffer (pH 7.0) as the mobile phase. The diode detector was set at 532 nm to quantify the malondialdehyde (MDA)-TBA adduct. An ex vivo tape stripping method was applied in 10 volunteers in three pre-defined regions of the volar forearms: the control; the irradiated; and the site containing the sunscreen (2.0 mg·cm−2). Ten adhesive tapes per region were used for SC removal. An exclusive ex vivo protocol to measure SC lipid peroxidation was preliminarily developed with linearity and selectivity. The protocol suggested the use of an artificial irradiation dose (5506 KJ·m−2) to improve the assay response from the SC. The sunscreen system had a significative decrease in SC lipoperoxidative damage compared to the control. Our protocol can aid in the efficacy establishment of anti-UV and antioxidant agents, for instance, in studies that aim at elucidating the level of SC lipid peroxidation and even in carrying out baseline investigations characterizing different ethnicities and genders. View Full-Text
Keywords: stratum corneum oxidative stress; HPLC; TBARS; tape stripping stratum corneum oxidative stress; HPLC; TBARS; tape stripping
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MDPI and ACS Style

Sauce, R.; Pinto, C.A.S.d.O.; Ayala-Jara, C.; Prieto, Z.A.; Velasco, M.V.R.; Baby, A.R. Preliminary Protocol Development of a HPLC-TBARS-EVSC (Ex Vivo Stratum Corneum) Assay for Skin Research: Application in a Sunscreen System. Sci. Pharm. 2021, 89, 17. https://0-doi-org.brum.beds.ac.uk/10.3390/scipharm89020017

AMA Style

Sauce R, Pinto CASdO, Ayala-Jara C, Prieto ZA, Velasco MVR, Baby AR. Preliminary Protocol Development of a HPLC-TBARS-EVSC (Ex Vivo Stratum Corneum) Assay for Skin Research: Application in a Sunscreen System. Scientia Pharmaceutica. 2021; 89(2):17. https://0-doi-org.brum.beds.ac.uk/10.3390/scipharm89020017

Chicago/Turabian Style

Sauce, Rafael; Pinto, Claudinéia A.S.d.O.; Ayala-Jara, Carmen; Prieto, Zulita A.; Velasco, Maria V.R.; Baby, André R. 2021. "Preliminary Protocol Development of a HPLC-TBARS-EVSC (Ex Vivo Stratum Corneum) Assay for Skin Research: Application in a Sunscreen System" Sci. Pharm. 89, no. 2: 17. https://0-doi-org.brum.beds.ac.uk/10.3390/scipharm89020017

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