Effect of Cadmium and Copper Exposure on Growth, Physio-Chemicals and Medicinal Properties of Cajanus cajan L. (Pigeon Pea)
Abstract
:1. Introduction
2. Results
2.1. Plant Growth Attritubutes
2.2. Chlorophyll Content and Photosynthetic Parameters
2.3. Flavonoid, Phenolic, and Proline Contents
2.4. Medicinal Properties (Anti-Bacterial, DPPH, and FRAP Activity)
2.5. Oxidative Stresses
2.6. Antioxidant Enzyme Response
2.7. Metal (Cd and Cu) Accumulation
2.8. Nutrient Uptake
3. Discussion
4. Materials and Methods
4.1. Material and Growth Conditions
4.2. Soil Preparation and Experimental Designs
4.3. Determination of Gaseous Exchange
4.4. Chlorophyll a and b Determination
4.5. Determination of Proline Contents
4.6. Determination of Total Phenolics and Flavonoids
4.7. DPPH Free Radical Scavenging Activity
4.8. FRAP (Ferric-Reducing Antioxidant Power) Assay
4.9. Anti-Bacterial Assay
4.10. MDA Contents
4.11. Hydrogen Peroxide (H2O2)
4.12. Electrolyte Leakage
4.13. Antioxidant Enzyme Extraction
4.14. Enzyme Determinations
4.15. Elemental Analysis
4.16. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
List of Abbreviations and Acronyms
References
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Treatment | Roots | Shoots | ||||
---|---|---|---|---|---|---|
Fresh Weight (g) | Dry Weight (g) | Length (cm) | Fresh Weight (g) | Dry Weight (g) | Length (cm) | |
Control | 5.55 ± 1.0 d | 2.61 ± 0.23 d | 23.40 ± 1.1 d | 9.45 ± 0.55 b | 5.11 ± 0.43 d | 91.40 ± 1.15 d |
Cd 20 + Cu 30 mg/kg | 5.10 ± 0.99 c | 2.42 ± 0.60 c | 21.40 ± 0.85 c | 8.75 ± 0.77 b | 4.91 ± 0.89 c | 86.10 ± 0.85 c |
Cd 40 + Cu 60 mg/kg | 4.72 ± 0.7 b | 2.11 ± 0.32 b | 18.45 ± 0.77 b | 7.11 ± 0.01 a | 4.01 ± 1.02 b | 80.50 ± 0.5 b |
Cd 60 + Cu 90 mg/kg | 3.92 ± 1.23 a | 1.82 ± 0.94 a | 13.45 ± 0.9 a | 6.21 ± 0.5 a | 3.21 ± 0.93 a | 73.40 ± 0.23 a |
Treatment | Flavonoids (mg/g DM GAE) | Phenols (mg/g DM Rutin) | Proline (µg/g FW) |
---|---|---|---|
Control | 32.35 ± 0.34 c | 55.51 ± 0.91 c | 20.43 ± 0.15 a |
Cd 20 + Cu 30 mg/kg | 42.40 ± 0.12 d | 68.53 ± 0.21 d | 45.41 ± 0.18 b |
Cd 40 + Cu 60 mg/kg | 26.45 ± 0.39 b | 45.42 ± 0.45 b | 70.40 ± 0.71 c |
Cd 60 + Cu 90 mg/kg | 17.41 ± 0.78 a | 32.46 ± 0.81 a | 90.47 ± 0.12 d |
Antibacterial Activities | Inhibition Zone (mm) | ||
---|---|---|---|
Gram-Positive Bacteria | Gram-Negative Bacteria | ||
S. aureus | E. coli | S. thyphi | |
Control | 11.60 ± 0.86 c | 10.52 ± 0.57 c | 12.36 ± 0.97 c |
Cd 20 + Cu 30mg/kg | 12.93 ± 0.34 d | 11.22 ± 0.77 d | 13.75 ± 0.58 d |
Cd 40 + Cu 60 mg/kg | 10.10 ± 0.57 b | 9.92 ± 0.33 b | 10.91 ± 0.11 b |
Cd 60 + Cu 90mg/kg | 9.65 ± 0.83 a | 8.44 ± 0.45 a | 8.21 ± 0.63 a |
Ciprofloxacin | 17.61 ± 0.88 e | 19.59 ± 0.71 e | 21.29 ± 0.77 e |
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Hayat, K.; Khan, A.; Bibi, F.; Salahuddin; Murad, W.; Fu, Y.; Batiha, G.E.-S.; Alqarni, M.; Khan, A.; Al-Harrasi, A. Effect of Cadmium and Copper Exposure on Growth, Physio-Chemicals and Medicinal Properties of Cajanus cajan L. (Pigeon Pea). Metabolites 2021, 11, 769. https://0-doi-org.brum.beds.ac.uk/10.3390/metabo11110769
Hayat K, Khan A, Bibi F, Salahuddin, Murad W, Fu Y, Batiha GE-S, Alqarni M, Khan A, Al-Harrasi A. Effect of Cadmium and Copper Exposure on Growth, Physio-Chemicals and Medicinal Properties of Cajanus cajan L. (Pigeon Pea). Metabolites. 2021; 11(11):769. https://0-doi-org.brum.beds.ac.uk/10.3390/metabo11110769
Chicago/Turabian StyleHayat, Khizar, Asif Khan, Farkhanda Bibi, Salahuddin, Waheed Murad, Yujie Fu, Gaber El-Saber Batiha, Mohammed Alqarni, Ajmal Khan, and Ahmed Al-Harrasi. 2021. "Effect of Cadmium and Copper Exposure on Growth, Physio-Chemicals and Medicinal Properties of Cajanus cajan L. (Pigeon Pea)" Metabolites 11, no. 11: 769. https://0-doi-org.brum.beds.ac.uk/10.3390/metabo11110769