Loss of Planar Cell Polarity Effector Fuzzy Causes Renal Hypoplasia by Disrupting Several Signaling Pathways
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mouse Breeding and Genotyping
2.2. Tissue Preparation
2.3. Immunofluorescence
2.4. Hematoxylin and Eosin Staining
2.5. TUNEL Assay
2.6. In Situ Hybridization
2.7. Analysis of Early Nephrogenesis
2.8. RNAseq and Bioinformatic Analysis
2.9. Quantitative Polymerase Chain Reaction
2.10. Statistical Analysis
3. Results
3.1. Morphological Analysis of Mutant Fuzzy−/− Kidneys
3.2. Analysis of the Gdnf/c-Ret Signaling Axis in Fuzzy−/−Embryonic Kidneys
3.3. Unbiased Analysis of Transcriptional Profiling in Fuzzy−/− vs. Wildtype Kidneys
3.4. Ciliogenesis and Shh Signaling in Fuzzy−/− Kidney
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Wang, I.-Y.; Chung, C.-F.; Babayeva, S.; Sogomonian, T.; Torban, E. Loss of Planar Cell Polarity Effector Fuzzy Causes Renal Hypoplasia by Disrupting Several Signaling Pathways. J. Dev. Biol. 2022, 10, 1. https://0-doi-org.brum.beds.ac.uk/10.3390/jdb10010001
Wang I-Y, Chung C-F, Babayeva S, Sogomonian T, Torban E. Loss of Planar Cell Polarity Effector Fuzzy Causes Renal Hypoplasia by Disrupting Several Signaling Pathways. Journal of Developmental Biology. 2022; 10(1):1. https://0-doi-org.brum.beds.ac.uk/10.3390/jdb10010001
Chicago/Turabian StyleWang, Irene-Yanran, Chen-Fang Chung, Sima Babayeva, Tamara Sogomonian, and Elena Torban. 2022. "Loss of Planar Cell Polarity Effector Fuzzy Causes Renal Hypoplasia by Disrupting Several Signaling Pathways" Journal of Developmental Biology 10, no. 1: 1. https://0-doi-org.brum.beds.ac.uk/10.3390/jdb10010001