MARCO+ Macrophage Dynamics in Regenerating Liver after 70% Liver Resection in Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mice
2.2. Animal Model
2.3. Isolation of Macrophages
2.4. Flow Cytometry Assay
2.5. Cell Culture of Macrophages
2.6. Activation of Macrophage Cell Cultures with LPS
2.7. Immunocytochemistry and Immunohistochemistry
2.8. Reverse Transcription Polymerase Chain Reaction (RT PCR) Gene Expression Assay
2.9. Immunoblotting
2.10. Statistics
3. Results
3.1. Macrophage Population Dynamics in Regenerating Livers
3.2. Transcriptional Dynamics of Macrophage Marker-Encoding Genes in Regenerating Livers
3.3. Comparative Analysis of Lipopolysaccharide (LPS) Effects on the Expression of Macrophage Markers in Bone Marrow-Derived Macrophages and Kupffer Cells
3.4. Protein Expression Dynamics for CD68, TIM4, and MARCO in Total Tissues and Macrophages of Regenerating Livers
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Tim4 | for | ATTAACAACAAGTGAGTCTTTGCAG |
rev | CTTCAGTGTTGTCTGGCCTCT | |
Marco | for | CCAGGACTTTCAGGTGCCAA |
rev | TGGCCAGAAGACCCTTTCAT | |
Clec4f | for | TGCAGGACAGGCTTTAGGAA |
rev | TGACTTAGGCCCCAGTCCTTG | |
Clec1b | for | GTTGGATTGGATTATCACGCCAG |
rev | TCCCAGAAAGATTAATCCCGTT | |
Gapdh | for | AGGCCGGTGCTGAGTATGTC |
rev | TGCCTGCTTCACCACCTTCT |
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Elchaninov, A.; Lokhonina, A.; Vishnyakova, P.; Soboleva, A.; Poltavets, A.; Artemova, D.; Makarov, A.; Glinkina, V.; Goldshtein, D.; Bolshakova, G.; et al. MARCO+ Macrophage Dynamics in Regenerating Liver after 70% Liver Resection in Mice. Biomedicines 2021, 9, 1129. https://0-doi-org.brum.beds.ac.uk/10.3390/biomedicines9091129
Elchaninov A, Lokhonina A, Vishnyakova P, Soboleva A, Poltavets A, Artemova D, Makarov A, Glinkina V, Goldshtein D, Bolshakova G, et al. MARCO+ Macrophage Dynamics in Regenerating Liver after 70% Liver Resection in Mice. Biomedicines. 2021; 9(9):1129. https://0-doi-org.brum.beds.ac.uk/10.3390/biomedicines9091129
Chicago/Turabian StyleElchaninov, Andrey, Anastasia Lokhonina, Polina Vishnyakova, Anna Soboleva, Anastasiya Poltavets, Daria Artemova, Andrey Makarov, Valeria Glinkina, Dmitry Goldshtein, Galina Bolshakova, and et al. 2021. "MARCO+ Macrophage Dynamics in Regenerating Liver after 70% Liver Resection in Mice" Biomedicines 9, no. 9: 1129. https://0-doi-org.brum.beds.ac.uk/10.3390/biomedicines9091129