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Article

A Multi-Scale Approach to Modeling the Interfacial Reaction Kinetics of Lipases with Emphasis on Enzyme Adsorption at Water-Oil Interfaces

1
Chair of Chemical and Process Engineering, Technical University of Berlin, Ackerstrasse 76, 13355 Berlin, Germany
2
HTW Berlin, Department of Engineering II, School of Life Science Engineering, Wilhelminenhofstr. 75A, 12459 Berlin, Germany
*
Author to whom correspondence should be addressed.
Received: 11 August 2020 / Revised: 22 August 2020 / Accepted: 24 August 2020 / Published: 2 September 2020
(This article belongs to the Special Issue Advanced Chemical Reaction Kinetics of Pharmaceutical Processes)
The enzymatic hydrolysis of triglycerides with lipases (EC 3.1.1.3.) involves substrates from both water and oil phases, with the enzyme molecules adsorbed at the water-oil (w/o) interface. The reaction rate depends on lipase concentration at the interface and the available interfacial area in the emulsion. In emulsions with large drops, the reaction rate is limited by the surface area. This effect must be taken into account while modelling the reaction. However, determination of the interfacial saturation is not a trivial matter, as enzyme molecules have the tendency to unfold on the interface, and form multi-layer, rendering many enzyme molecules unavailable for the reaction. A multi-scale approach is needed to determine the saturation concentration with specific interfacial area so that it can be extrapolated to droplet swarms. This work explicitly highlights the correlation between interfacial adsorption and reaction kinetics, by integration of the adsorption kinetics into the enzymatic reaction. The rate constants were fitted globally against data from both single droplet and drop swarm experiments. The amount of adsorbed enzymes on the interface was measured in a single drop with a certain surface area, and the enzyme interfacial loading was estimated by Langmuir adsorption isotherm. View Full-Text
Keywords: enzymatic hydrolysis; lipases; interfacial kinetics modeling; protein adsorption enzymatic hydrolysis; lipases; interfacial kinetics modeling; protein adsorption
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MDPI and ACS Style

Rusli, S.; Grabowski, J.; Drews, A.; Kraume, M. A Multi-Scale Approach to Modeling the Interfacial Reaction Kinetics of Lipases with Emphasis on Enzyme Adsorption at Water-Oil Interfaces. Processes 2020, 8, 1082. https://0-doi-org.brum.beds.ac.uk/10.3390/pr8091082

AMA Style

Rusli S, Grabowski J, Drews A, Kraume M. A Multi-Scale Approach to Modeling the Interfacial Reaction Kinetics of Lipases with Emphasis on Enzyme Adsorption at Water-Oil Interfaces. Processes. 2020; 8(9):1082. https://0-doi-org.brum.beds.ac.uk/10.3390/pr8091082

Chicago/Turabian Style

Rusli, Sherly, Janna Grabowski, Anja Drews, and Matthias Kraume. 2020. "A Multi-Scale Approach to Modeling the Interfacial Reaction Kinetics of Lipases with Emphasis on Enzyme Adsorption at Water-Oil Interfaces" Processes 8, no. 9: 1082. https://0-doi-org.brum.beds.ac.uk/10.3390/pr8091082

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